A Light Challenge: First Graders Engineer an Obstacle Course with Flashlights

In this class, we wanted to support students’ knowledge of light and the ways in which it interacts with other materials through a series of connected learning experiences. These experiences are related to NGSS standard 1-PS4-3 Waves and Their Applications in Technologies for Information Transfer. Plan and conduct investigations to determine the effect of placing objects made with different materials in the path of a beam of light. We aimed for children to discover that light tends to move in a straight line unless something gets in the way, materials can affect the beam of light, and the way light moves can be impacted by moving the objects. The connected activities described here were guided by the 5E learning cycle. The students watched the video to spark their curiosity about the path in which something might travel, worked in teams to design and test ideas while exploring the properties of the light, and documented and communicated their observations

Teaching Early and Elementary STEM

This Open Access Educational textbook, Teaching Early and Elementary STEM , was written to support pre-service early childhood and elementary teachers in their journey to become facilitators of science, technology, engineering, and math, or “STEM,” and integrated STEM in their future classrooms. Students who read and use this text will deepen their understanding of “STEM” and “integrated STEM,” learn what early childhood and elementary students need to know and be able to do in relation to STEM, and understand ways to create activity plans and implement current research-based approaches to teaching and pedagogy. This text arose out of our Early/Elementary STEM Collaboration project, which started in 2017 with the intention of increasing the quality of teacher preparation in STEM across early childhood and elementary education. The team is composed of math and science education professors, classroom in-service teachers, and pre-service teachers in pre-school through fifth grade. We are driven by the values of collaboration, strengths-based approaches to teaching and learning, constructivist philosophy of teaching and learning, and applied STEM experiences to increase access and equity. Our model of preparing pre-service teachers has been published elsewhere in more detail (Robertson, Nivens, & Lange, 2019). We built this open access product to include the following: 1) completely new content that includes input from our team as well as examples of integrated STEM learning experiences; 2) adaptations of existing resources, and; 3) compilations of existing free resources (e.g., Next Generation Science Standards).https://dc.etsu.edu/etsu-oer/1007/thumbnail.jp

Bone marrow stromal cells attenuate sepsis via prostaglandin E2— dependent reprogramming of host macrophages to increase their interleukin-10 production

Sepsis causes over 200,000 deaths yearly in the US; better treatments are urgently needed. Administering bone marrow stromal cells (BMSCs—also known as mesenchymal stem cells) to mice before or shortly after inducing sepsis by cecal ligation and puncture reduced mortality and improved organ function. The beneficial effect of BMSCs was eliminated by macrophage depletion or pretreatment with antibodies specific for interleukin-10 (IL-10) or IL-10 receptor. Monocytes and/ or macrophages from septic lungs made more IL-10 when prepared from mice treated with BMSCs versus untreated mice. Lipopolysaccharide (LPS)-stimulated macrophages produced more IL-10 when cultured with BMSCs, but this effect was eliminated if the BMSCs lacked the genes encoding Toll-like receptor 4, myeloid differentiation primary response gene-88, tumor necrosis factor (TNF) receptor-1a or cyclooxygenase-2. Our results suggest that BMSCs (activated by LPS or TNF-α) reprogram macrophages by releasing prostaglandin E2 that acts on the macrophages through the prostaglandin EP2 and EP4 receptors. Because BMSCs have been successfully given to humans and can easily be cultured and might be used without human leukocyte antigen matching, we suggest that cultured, banked human BMSCs may be effective in treating sepsis in high-risk patient groups.Sepsis, a serious medical condition that affects 18 million people per year worldwide, is characterized by a generalized inflammatory state caused by infection. Widespread activation of inflammation and coagulation pathways progresses to multiple organ dysfunction, collapse of the circulatory system (septic shock) and death. Because as many people die of sepsis annually as from acute myocardial infarction1, a new treatment regimen is desperately needed. In the last few years, it has been discovered that BMSCs are potent modulators of immune responses2-5. We wondered whether such cells could bring the immune response back into balance, thus attenuating the underlying pathophysiology that eventually leads to severe sepsis, septic shock and death6,7. As a model of sepsis, we chose cecal ligation and puncture (CLP), a procedure that has been used for more than two decades8. This mouse model closely resembles the human disease: it has a focal origin (cecum), is caused by multiple intestinal organisms, and results in septicemia with release of bacterial toxins into the circulation. With no treatment, the majority of the mice die 24-48 h postoperatively. Originally published Nature Medicine, Vol. 15, No. 1, Jan 200

Transcriptome Analysis Reveals Strain-Specific and Conserved Stemness Genes in Schmidtea mediterranea

The planarian Schmidtea mediterranea is a powerful model organism for studying stem cell biology due to its extraordinary regenerative ability mediated by neoblasts, a population of adult somatic stem cells. Elucidation of the S. mediterranea transcriptome and the dynamics of transcript expression will increase our understanding of the gene regulatory programs that regulate stem cell function and differentiation. Here, we have used RNA-Seq to characterize the S. mediterranea transcriptome in sexual and asexual animals and in purified neoblast and differentiated cell populations. Our analysis identified many uncharacterized genes, transcripts, and alternatively spliced isoforms that are differentially expressed in a strain or cell type-specific manner. Transcriptome profiling of purified neoblasts and differentiated cells identified neoblast-enriched transcripts, many of which likely play important roles in regeneration and stem cell function. Strikingly, many of the neoblast-enriched genes are orthologs of genes whose expression is enriched in human embryonic stem cells, suggesting that a core set of genes that regulate stem cell function are conserved across metazoan species

Exploring a Preschool STEM Professional Development Approach in the Context of the Garden

Participants will explore a research-based approach to STEM professional development for preschool educators using hands-on explorations and rich discussions in the context of the garden

Technology, Instructional Methods, and the Systemic Messiness of Innovation: Improving Reading Fluency for Low Socio‑Economic Elementary School Students

Reading fluency—the ability to read accurately, with appropriate pacing, expression, and rhythm—is a fundamental skill for elementary school students to develop (Snow et al. 1998). Reading fluency is related to comprehension skills (Daane et al. 2005; Donahue et al. 1999; Pinnell et al. 1995) and to overall educational achievement (Silberglitt et al. 2006). However, many elementary school students do not reach grade-level reading fluency levels (Hemphill and Vanneman 2011; Pinnell et al. 1995), especially those from low-resource communities or from racial and ethnic minority groups (Donahue et al. 1999; Hemphill and Vanneman 2011). Research has suggested that children who do not develop the ability to read fluently early in the schooling process are likely to experience difficulty learning and comprehending important material from texts introduced in later grades (Chall et al. 1990; Lyon and Moats 1997; Rasinski et al. 2012). Interventions can be effective in improving reading fluency..

Show Me 5: Building Number Composition Skills in Preschool

“How many different ways can you show me 5 on your fingers?” This hands-on workshop will dive into number operations, especially focused on number composition. We will discuss what number composition is, how it develops for children during preschool, and how best to encourage children’s thinking and doing around this topic. We will consider strategies, view videos, and explore materials and activities that can meaningfully support children to make sense of number relationships