Normal phase HPLC profiling of the acetylcholinesterase activity in apolar plant extracts

Among nineteen evaluated Clusiaceous species, one stem bark CH2Cl2 crude extract was selected based on a significant inhibition of acetylcholinesterase (AChE) using the micro-dilution Ellman\u27s method [1]. A normal phase HPLC profiling with micro-fractionation of this extract provided discrete fractions every 20 seconds. In order to obtain a comprehensive profiling of AChE activity all microfractions were tested [2] in dilution assay (Ellman) as well as by bioautography (the Fast Blue B salt method). Furthermore the potency of inhibition was evaluated both by keeping the genuine concentration within the extract and after normalisation to a standard concentration level. From the active fractions five pure compounds were isolated and identified. The different methods of sample preparation and biological evaluation associated with normal-phase micro-fractionation of plant extracts are critically discussed

Synthesis of new 1- 2-azido-2-(2,4-dichlorophenyl)ethyl -1H/-imidazoles and in vitro evaluation of their antifungal activity

International audienc

Return to sport soccer after anterior cruciate ligament reconstruction: ISAKOS consensus

Many factors can affect the return to pivoting sports, after an Anterior Cruciate Ligament Reconstruction. Prehabilitation, rehabilitation, surgical and psychological aspects play an essential role in the decision to return to sports. The purpose of this study is to reach an international consensus about the best conditions for returning to sports in soccer-one of the most demanding level I pivoting sports after anterior cruciate ligament (ACL) reconstruction

A microsystem for air quality measurement in future housings

International audienceNowadays, about half of humanity is housed in cities, a number expected to rise up to more than 2/3 by 2050 . Whether such a prediction is accurate is not relevant, we already experience the major issue that breathing a reasonably pure indoor air is becoming. Analytical chemistry has thus tremendous challenges ahead, among which the ability to design sensing elements for continuous air quality monitoring in closed environments. Functional materials with specific optimized properties are a growing part of modern chemistry and such materials may lead to disruptive solution in the field of analytical chemistry. For instance, hybrid porous materials prepared by the Sol-Gel process may be turned into functional sensing materials through careful formulation and process control . Incorporating such materials into microsystems is one promising way to new sensing approaches but their integration for sensing purposes into microsystems is far from being straightforward, which probably explains that it has not been reported yet. We have developed a microsystem based on an innovative microfluidic design and functional materials integrations that allows for gas detection and is compatible with continuous air quality measurements. First, we will describe the specificity of its design concerning micro-reactors fabrication and sensing capability. The preparation of the microfluidic device from micro-milled brass molds, and the method for fast and neat preparation of hundreds of individual micro-reactors will be reviewed. Then, the keys steps necessary to integrate functional materials into this device, such as the control of gelation will be explained. Special emphasis will be put on the interesting materials behavior within the microsystem. Finally, we will demonstrate the ability of our microsystem to perform quantitative optical detection of one of the main indoor air pollutants: formaldehyde . Based on air quality regulation, we will discuss the possibility of using such air quality monitoring microsystems in housings