Dissection of ancestral genetic contributions to Creole goat populations

Goats have played a key role as source of nourishment for humans in their expansion all over the world in long land and sea trips. This has guaranteed a place for this species in the important and rapid episode of livestock expansion triggered by Columbus’ arrival in the Americas in the late 1400s. The aims of this study are to provide a comprehensive perspective on genetic diversity in American goat populations and to assess their origins and evolutionary trajectories. This was achieved by combining data from autosomal neutral genetic markers obtained in more than two thousand samples that encompass a wide range of Iberian, African and Creole goat breeds. In general, even though Creole populations differ clearly from each other, they lack a strong geographical pattern of differentiation, such that populations of different admixed ancestry share relatively close locations throughout the large geographical range included in this study. Important Iberian signatures were detected in most Creole populations studied, and many of them, particularly the Cuban Creole, also revealed an important contribution of African breeds. On the other hand, the Brazilian breeds showed a particular genetic structure and were clearly separated from the other Creole populations, with some influence from Cape Verde goats. These results provide a comprehensive characterisation of the present structure of goat genetic diversity, and a dissection of the Iberian and African influences that gave origin to different Creole caprine breeds, disentangling an important part of their evolutionary history. Creole breeds constitute an important reservoir of genetic diversity that justifies the development of appropriate management systems aimed at improving performance without loss of genomic diversity

Practical issues in early switching from intravenous to oral antibiotic therapy in children with uncomplicated acute hematogenous osteomyelitis: Results from an italian survey

Background: The European Society of Pediatric Infectious Diseases (ESPID) guidelines for acute hematogenous osteomyelitis (AHOM) have been published recently. In uncomplicated cases, an early (2-4 days) switch to oral empirical therapy, preferentially with flucloxacillin, is recommended in low methicillin-resistant Staphylococcus aureus settings. We conducted a survey with the aim of evaluating the behaviors of Italian pediatricians at this regard. Methods: An open-ended questionnaire investigating the empiric therapy adopted in uncomplicatedAHOMchildren according to age was sent by email to 31 Italian pediatric clinics taking care of children with infectious diseases, and results were analyzed. Results: The preferred intravenous (IV) regimen was a penicillin plus an aminoglycoside (n = 10; 32.3%) in children aged <3 months, and a combination of a third-generation cephalosporin plus oxacillin (n = 7; 22.6%), or oxacillin alone (n = 6; 19.4%) in those 653 months. In every age class, amoxicillin-clavulanate was the first-choice oral antibiotic. Other antibiotics largely used orally included clindamycin, rifampicin, and trimethoprim/sulfamethoxazole. Flucloxacillin was never prescribed. Only 3 centers switched to oral therapy within 7 days in children 653 months of age. The most commonly reported reason influencing the time to switch to oral therapy concerned caregivers\u2019 adherence to oral therapy. Conclusion: Adherence to guidelines was poor, and early transition to oral therapy in the clinical practice was rarely adopted. Given the large use of potentially effective, but poorly studied, oral antibiotics such as amoxicillin/clavulanate, trimethoprim/sulfamethoxazole, and rifampicin, our data may stimulate further studies of this regard

Phenotypic characterization of the goat population of Santa Elena province (Ecuador)

It was proposed to typify phenotypically the goats of the Santa Elena province, Ecuador, and to test if there were different phenotypes. 344 animals were sampled in different types of production systems. The following variables were observed: a) morphological: coat color pattern and coat color (CCP, CC); fur and fur characteristics (FUR, CHF); pigmentation of skin, mucous membranes and hooves (SP, PMM, HP); size and arrangement of ears (ES, EO); facial profile (FP); mamellas (M); beard (B); type and orientation of horns (HT, HO); shape of rump (SR); b) morpho-structural: length and width head (HL, HW); face length (FL); ear length (EL); shoulder width (SW); depth and girth chest (CHD, CHG); cannon perimeter (CP); body length (BL); height at withers (HAW); length and width of rump (RL, RW). The multivariate analyzes performed showed 3 groups: 1) small animals; CC: red (35%), pied (33%); PMM: pigmented (43%); B: presence (44%); FP: straight (83%); ES: small (89%); EO: horizontal (47%), erect (42%); HT: arched (68%); SR: slight slope (89%). 2) wider and longer animals; medium height; CC: black (38%), red (35%); FP: straight (55%), slight convex (34%); ES: small (37%), medium (38%); EO: horizontal, erect and pendolous (84%); HT: arched (58%), curved (38%); SR: slight slope (71%). 3) taller animals; greater RL and BW; PMM: pigmented (24%); B: presence (19%); CC: red (41%), black (36%); FP: slight convex (58%), straight (39%); ES: long (51%), pendolous (64%); HT: arched (45%), straight (43%); SR: slight slope (75%). Group 1 corresponded to the oldest biotype of Santa Elena that would represent the Creole phenotype, the other two would be the result of crossings due to introductions of other races coming mainly from Loja (southern Ecuador) and Peru.Se propuso tipificar fenotípicamente a las cabras de la provincia de Santa Elena, Ecuador, y probar si existían diferentes fenotipos. Se muestrearon 344 animales en distintos tipos de sistemas productivos y se observaron las siguientes variables: a) morfológicas: patrón y color de capa (PCA, CCA); pelaje y características del pelaje (PEL, CPEL); pigmentación de piel, mucosas y pezuñas (PPI, PMU, PPE); tamaño y disposición de orejas (TOR, DOR); perfil craneal (PCR); mamelas (MAM); barba (BAR); tipo y orientación de cuernos (TCU, OCU); grupa (GRU); b) morfo-estructurales: longitud y ancho de cabeza (LCB, ACB); largo de cara (LCA); longitud de oreja (LOR); ancho de hombros (AHO); profundidad y perímetro del tórax (PRT, PET); perímetro de caña (PC); longitud del cuerpo (LCR); altura a la cruz (ACR); longitud y ancho de grupa (LGR, AGR). Los análisis multivariados mostraron 3 grupos: 1) animales pequeños; CCA: colorada (35%), variada (33%); PMU (43%); BAR (44%); PCR recto (83%); TOR pequeñas (89%); DOR: horizontal (47%), hacia adelante (42%); TCU arqueados (68%); GRU semiplana (89%). 2) animales más anchos y largos; altura mediana; CCA: negra (38%), colorada (35%); PCR: recto (55%), subconvexo (34%); TOR: pequeñas (37%), medianas (38%); DOR: horizontal, hacia adelante y caídas (84%); TCU: arqueados (58%), curvos (38%); GRU semiplana (71%). 3) animales más altos; mayor LGR y PC; PMU (25%); BAR (19%); CCA: colorada (42%), negra (36%); PCR: subconvexo (58%), recto (39%); TOR: largas (51%), caídas (64%); TCU: arqueados (45%), rectos (43%); GRU semiplana (75%). El Grupo 1 correspondió al biotipo más antiguo de Santa Elena que representaría el fenotipo Criollo, los otros dos, serían el resultado de cruzamientos debidos a introducciones de otras razas procedentes principalmente de Loja (sur de Ecuador) y Perú

Dissection of ancestral genetic contributions to Creole goat populations

Goats have played a key role as source of nourishment for humans in their expansion all over the world in long land and sea trips. This has guaranteed a place for this species in the important and rapid episode of livestock expansion triggered by Columbus' arrival in the Americas in the late 1400s. The aims of this study are to provide a comprehensive perspective on genetic diversity in American goat populations and to assess their origins and evolutionary trajectories. This was achieved by combining data from autosomal neutral genetic markers obtained in more than two thousand samples that encompass a wide range of Iberian, African and Creole goat breeds. In general, even though Creole populations differ clearly from each other, they lack a strong geographical pattern of differentiation, such that populations of different admixed ancestry share relatively close locations throughout the large geographical range included in this study. Important Iberian signatures were detected in most Creole populations studied, and many of them, particularly the Cuban Creole, also revealed an important contribution of African breeds. On the other hand, the Brazilian breeds showed a particular genetic structure and were clearly separated from the other Creole populations, with some influence from Cape Verde goats. These results provide a comprehensive characterisation of the present structure of goat genetic diversity, and a dissection of the Iberian and African influences that gave origin to different Creole caprine breeds, disentangling an important part of their evolutionary history. Creole breeds constitute an important reservoir of genetic diversity that justifies the development of appropriate management systems aimed at improving performance without loss of genomic diversity.Depto. de Producción AnimalFac. de VeterinariaTRUEpu