276 research outputs found
Ageing has no effect on the regulation of the ubiquitin proteasome-related genes and proteins following resistance exercise
Skeletal muscle atrophy is a critical component of the ageing process. Age-related muscle wasting is due to disrupted muscle protein turnover, a process mediated in part by the ubiquitin proteasome pathway (UPP). Additionally, older subjects have been observed to have an attenuated anabolic response, at both the molecular and physiological levels, following a single-bout of resistance exercise (RE). We investigated the expression levels of the UPP-related genes and proteins involved in muscle protein degradation in 10 older (60-75 years) versus 10 younger (18-30 years) healthy male subjects at basal as well as 2 hours after a single-bout of RE. MURF1, atrogin-1 and FBXO40, their substrate targets PKM2, myogenin, MYOD, MHC and EIF3F as well as MURF1 and atrogin-1 transcriptional regulators FOXO1 and FOXO3 gene and/or protein expression levels were measured via real time PCR and western blotting, respectively. At basal, no age-related difference was observed in the gene/protein levels of atrogin-1, MURF1, myogenin, MYOD and FOXO1/3. However, a decrease in FBXO40 mRNA and protein levels was observed in older subjects, while PKM2 protein was increased in older subjects. In response to RE, MURF1, atrogin-1 and FBXO40 mRNA were upregulated in both the younger and older subjects, with changes observed in protein levels. In conclusion, UPP-related gene/protein expression is comparably regulated in healthy young and old male subjects at basal and following RE. These findings suggest that UPP signalling plays a limited role in the process of age-related muscle wasting. Future studies are required to investigate additional proteolytic mechanisms in conjunction with skeletal muscle protein breakdown measurements following RE in older versus younger subjects
Simulation model for the study of overhead rail current collector systems dynamics, focused on the design of a new conductor rail
Overhead rigid conductor arrangements for current collection for railway traction have some advantages compared to other, more conventional, energy supply systems. They are simple, robust and easily maintained, not to mention their flexibility as to the required height for installation, which makes them particularly suitable for use in subway infrastructures. Nevertheless, due to the increasing speeds of new vehicles running on modern subway lines, a more efficient design is required for this kind of system.
In this paper, the authors present a dynamic analysis of overhead conductor rail systems focused on the design of a new conductor profile with a dynamic behaviour superior to that of the system currently in use. This means that either an increase in running speed can be attained, which at present does not exceed 110 km/h, or an increase in the distance between the rigid catenary supports with the ensuing saving in installation costs.
This study has been carried out using simulation techniques. The ANSYS programme has been used for the finite element modelling and the SIMPACK programme for the elastic multibody systems analysis
Influence d'interphases pyrocarbone déposées par CVI pulsée sur les caracteristiques mécaniques de matériaux composites unidirectionnels
The prospective kindergarten and elementary school teachers’ understanding of the ratio concept
Este artigo centra-se na compreensão que futuros educadores e professores dos primeiros anos de escolaridade evidenciam do conceito de razão. No estudo participaram 81 estudantes do 2º ano da Licenciatura em Educação Básica, de uma universidade portuguesa, aos quais foi aplicado um questionário, incidindo em quatro dimensões: significado de razão; usos do conceito de razão; representação simbólica de razão; e representações para explicação do conceito de razão. Em termos de resultados, as maiores frequências foram obtidas para a: ideia de razão como comparação/relação entre grandezas; crença em que o conceito de razão pode ser usado em contextos diversificados; crença em que pode ser representado através de operações envolvendo letras, números ou apenas os sinais das operações, quando é pedida a sua representação simbólica, e através de diagramas e representações gráficas, quando é solicitada a descrição da sua explicação. Estes resultados revelam fragilidades no conhecimento matemático dos estudantes, sobretudo no que se refere à definição do conceito de razão e às suas representações.This paper focuses on the prospective kindergarten and elementary school teachers’ understanding of the ratio
concept. Eighty-one undergraduates preparing to become kindergarten and primary school teachers, in a
Portuguese university, participated in the study. They answered to a questionnaire aiming at evaluating their
understanding of the ratio concept based on four dimensions: meaning of ratio; use of the ratio concept; symbolic representations of ratio; and representations for explaining the ratio concept. As far as results are concerned, the highest frequencies were obtained for: the idea of ratio as a comparison/relationship between two magnitudes; the belief that this concept is used in several contexts; the belief that it can be represented through operations with letters, figures or operation signals, as in symbolic representations; and through diagrams and graphical representations, as in representations for explaining the ratio concept. The results show weaknesses in prospective teachers’ mathematical knowledge of ratio, namely with regard to its definition and representations.Este trabalho contou com o apoio de Fundos Nacionais através da FCT – Fundação para a Ciência e a Tecnologia no âmbito do projecto PEst-OE/CED/UI1661/2014 do CIEd-UM.info:eu-repo/semantics/publishedVersio
High-Energy gamma-ray Astronomy and String Theory
There have been observations, first from the MAGIC Telescope (July 2005) and
quite recently (September 2008) from the FERMI Satellite Telescope, on
non-simultaneous arrival of high-energy photons from distant celestial sources.
In each case, the highest energy photons were delayed, as compared to their
lower-energy counterparts. Although the astrophysics at the source of these
energetic photons is still not understood, and such non simultaneous arrival
might be due to non simultaneous emission as a result of conventional physics
effects, nevertheless, rather surprisingly, the observed time delays can also
fit excellently some scenarios in quantum gravity, predicting Lorentz violating
space-time "foam" backgrounds with a non-trivial subluminal vacuum refractive
index suppressed linearly by a quantum gravity scale of the order of the
reduced Planck mass. In this pedagogical talk, I discuss the MAGIC and FERMI
findings in this context and I argue on a theoretical model of space-time foam
in string/brane theory that can accommodate the findings of those experiments
in agreement with all other stringent tests of Lorentz invariance. However, I
stress the current ambiguities/uncertainties on the source mechanisms, which
need to be resolved first before definite conclusions are reached regarding
quantum gravity foam scenarios.Comment: 34 pages latex, 12 eps figures incorporated, uses special macros.
Based on invited plenary talk at DICE 2008 Conference (Castiglioncello,
Italy), September 22-26 200
Stringy Space-Time Foam and High-Energy Cosmic Photons
In this review, I discuss briefly stringent tests of Lorentz-violating
quantum space-time foam models inspired from String/Brane theories, provided by
studies of high energy Photons from intense celestial sources, such as Active
Galactic Nuclei or Gamma Ray Bursts. The theoretical models predict
modifications to the radiation dispersion relations, which are quadratically
suppressed by the string mass scale, and time delays in the arrival times of
photons (assumed to be emitted more or less simultaneously from the source),
which are proportional to the photon energy, so that the more energetic photons
arrive later. Although the astrophysics at the source of these energetic
photons is still not understood, and such non simultaneous arrivals, that have
been observed recently, might well be due to non simultaneous emission as a
result of conventional physics effects, nevertheless, rather surprisingly, the
observed time delays can also fit excellently the stringy space-time foam
scenarios, provided the space-time defect foam is inhomogeneous. The key
features of the model, that allow it to evade a plethora of astrophysical
constraints on Lorentz violation, in sharp contrast to other field-theoretic
Lorentz-violating models of quantum gravity, are: (i) transparency of the foam
to electrons and in general charged matter, (ii) absence of birefringence
effects and (iii) a breakdown of the local effective lagrangian formalism.Comment: 26 pages Latex, 4 figures, uses special macros. Keynote Lecture in
the International Conference "Recent Developments in Gravity" (NEB14),
Ioannina (Greece) June 8-11 201
GLAST and Lorentz violation
We study possible Lorentz violations by means of gamma-ray bursts (GRB) with
special focus on the Large Array Telescope (LAT) of GLAST. We simulate bursts
with gtobssim and introduce a Lorentz violating term in the arrival times of
the photons. We further perturb these arrival times and energies with a
Gaussian distribution corresponding to the time resp. energy resolution of
GLAST. We then vary the photon flux in gtobssim in order to derive a relation
between the photon number and the standard deviation of the Lorentz violating
term. We conclude with the fact that our maximum likelihood method as first
developed in [1] is able to make a statement whether Nature breaks the Lorentz
symmetry if the number of bursts with known redshifts is of the order of 100.Comment: 13 pages, 8 figures and 2 tables, Accepted for publication by JCAP
after a couple of revision
Implementing virtual collaborative inquiry practises in a middle-school context
The aim of the present study was to investigate the challenges that relate to the implementation of virtual inquiry practises in middle school. The case was a school course in which a group of Finnish students (N = 14) and teachers (N = 7) completed group inquiries through virtual collaboration, using a web-based learning environment. The task was to accomplish a cross-disciplinary inquiry into cultural issues. The students worked mainly at home and took much responsibility for their course achievements. The investigators analysed the pedagogical design of the course and the content of the participants' interaction patterns in the web-based environment, using qualitative content analysis and social network analysis. The findings suggest that the students succeeded in producing distinctive cultural products, and both the students and the teachers adopted novel roles during the inquiry. The web-based learning environment was used more as a coordination tool for organizing the collaborative work than as a forum for epistemic inquiry. The tension between the school curriculum and the inquiry practises was manifest in the participants' discussions of the assessment criteria of the course.The aim of the present study was to investigate the challenges that relate to the implementation of virtual inquiry practises in middle school. The case was a school course in which a group of Finnish students (N = 14) and teachers (N = 7) completed group inquiries through virtual collaboration, using a web-based learning environment. The task was to accomplish a cross-disciplinary inquiry into cultural issues. The students worked mainly at home and took much responsibility for their course achievements. The investigators analysed the pedagogical design of the course and the content of the participants' interaction patterns in the web-based environment, using qualitative content analysis and social network analysis. The findings suggest that the students succeeded in producing distinctive cultural products, and both the students and the teachers adopted novel roles during the inquiry. The web-based learning environment was used more as a coordination tool for organizing the collaborative work than as a forum for epistemic inquiry. The tension between the school curriculum and the inquiry practises was manifest in the participants' discussions of the assessment criteria of the course.The aim of the present study was to investigate the challenges that relate to the implementation of virtual inquiry practises in middle school. The case was a school course in which a group of Finnish students (N = 14) and teachers (N = 7) completed group inquiries through virtual collaboration, using a web-based learning environment. The task was to accomplish a cross-disciplinary inquiry into cultural issues. The students worked mainly at home and took much responsibility for their course achievements. The investigators analysed the pedagogical design of the course and the content of the participants' interaction patterns in the web-based environment, using qualitative content analysis and social network analysis. The findings suggest that the students succeeded in producing distinctive cultural products, and both the students and the teachers adopted novel roles during the inquiry. The web-based learning environment was used more as a coordination tool for organizing the collaborative work than as a forum for epistemic inquiry. The tension between the school curriculum and the inquiry practises was manifest in the participants' discussions of the assessment criteria of the course.Peer reviewe
Discharge–calcium concentration relationships in streams of the Amazon and Cerrado of Brazil : soil or land use controlled
Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of springer for personal use, not for redistribution. The definitive version was published in Biogeochemistry 105 (2011): 19-35, doi:10.1007/s10533-011-9574-2.Stream discharge-concentration relationships are indicators of terrestrial ecosystem function. Throughout the Amazon and Cerrado regions of Brazil rapid changes in land use and land cover may be altering these hydrochemical relationships. The current analysis focuses on factors controlling the discharge-calcium (Ca) concentration relationship since previous research in these regions has demonstrated both positive and negative slopes in linear log10discharge-log10Ca concentration regressions. The objective of the current study was to evaluate factors controlling stream discharge-Ca concentration relationships including year, season, stream order, vegetation cover, land use, and soil classification. It was hypothesized that land use and soil class are the most critical attributes controlling discharge-Ca concentration relationships. A multilevel, linear regression approach was utilized with data from 28 streams throughout Brazil. These streams come from three distinct regions and varied broadly in watershed size (106 ha) and discharge (10-5.7 to 103.2 m3 sec-1). Linear regressions of log10Ca versus log10discharge in 13 streams have a preponderance of negative slopes with only two streams having significant positive slopes. An ANOVA decomposition suggests the effect of discharge on Ca concentration is large but variable. Vegetation cover, which incorporates aspects of land use, explains the largest proportion of the variance in the effect of discharge on Ca followed by season and year. In contrast, stream order, land use, and soil class explain most of the variation in stream Ca concentration. In the current data set, soil class, which is related to lithology, has an important effect on Ca concentration but land use, likely through its effect on runoff concentration and hydrology, has a greater effect on discharge-concentration relationships.This research was supported by grant #’s NCC5-686 and NNG06GE88A of NASA’s Terrestrial Ecology Program as part of the Large-scale Biosphere-Atmosphere Experiment in Amazonia (LBA-ECO) project
Bayesian Classification and Regression Trees for Predicting Incidence of Cryptosporidiosis
Background Classification and regression tree (CART) models are tree-based exploratory data analysis methods which have been shown to be very useful in identifying and estimating complex hierarchical relationships in ecological and medical contexts. In this paper, a Bayesian CART model is described and applied to the problem of modelling the cryptosporidiosis infection in Queensland, Australia. Methodology/Principal Findings We compared the results of a Bayesian CART model with those obtained using a Bayesian spatial conditional autoregressive (CAR) model. Overall, the analyses indicated that the nature and magnitude of the effect estimates were similar for the two methods in this study, but the CART model more easily accommodated higher order interaction effects. Conclusions/Significance A Bayesian CART model for identification and estimation of the spatial distribution of disease risk is useful in monitoring and assessment of infectious diseases prevention and control
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