The meat of goat kids and lambs as a possible source of Toxoplasma gondii for consumers

Toxoplasmosis is a worldwide zoonosis and the consumption of undercooked meat is a major risk factor for human infection. Antibodies to Toxoplasma gondii were found in 28.2% (11/39) and 27.8% (5/18) of home-slaughtered goat kids (n = 39) and lambs (n = 18) from four farms in the Czech Republic. The DNA of the parasite was detected in the tissues (diaphragm and/or lung) of 10.3% (4/39) of kids and 16.7% (3/18) of lambs. Although the consumption of kid and lamb meat is low in the Czech Republic, its consumption may become a source of Toxoplasma infection in humans

UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences

UDP-glycosyltransferases (UGT), catalysing conjugation of UDP-activated sugar donors to small lipophilic chemicals, are widespread in living organisms from bacteria to fungi, plant, or animals. The progress of genome sequencing has enabled an assessment of the UGT multigene family in Haemonchus contortus (family Trichostrongylidae, Nematoda), a hematophagous gastrointestinal parasite of small ruminants. Here we report 32 putative UGT genes divided into 15 UGT families. Phylogenetic analysis in comparison with UGTs from Caenorhabditis elegans, a free-living model nematode, revealed several single member homologues, a lack of the dramatic gene expansion seen in C. elegans, but also several families (UGT365, UGT366, UGT368) expanded in H. contortus only. The assessment of constitutive UGT mRNA expression in H. contortus adults identified significant differences between females and males. In addition, we compared the expression of selected UGTs in the drug-sensitive ISE strain to two benzimidazole-resistant strains, IRE and WR, with different genetic backgrounds. Constitutive expression of UGT368B2 was significantly higher in both resistant strains than in the sensitive strain. As resistant strains were able to deactivate benzimidazole anthelmintics via glycosylation more effectively then the sensitive strain, UGT368B2 enhanced constitutive expression might contribute to drug resistance in H. contortus

Sezónní migrace a zdravotní stav jelena lesního (Cervus elaphus) v Krkonošském národním parku

Cílem úkolu bylo především označení dostatečného množství jelení zvěře pro získání využitelného množství dat, zjištění zdravotního stavu označených jedinců, z čehož je možné posoudit zdravotní stav dané populace v celé oblasti a ve spolupráci se Správou KRNAP, po dodání dostatečných dat (záznamy pozorování označených jedinců, záznamy ulovených označených jedinců) vyhodnotit prostorovou aktivitu a migraci jelení zvěře v oblasti Krkonošského národního parku

Non-Linear Mechanical Behavior of Visco-Elastic Biological Structures – Measurements and Models

Mechanical properties of biological structures affect functional ability of organism. Current knowledge is prevailingly concentrated on static characteristics. The present work analyzed dynamic mechanical responses of various biological materials. Following biological structures were measured: samples of aorta walls of human origin and from model organisms, human body surface, and samples of bones of various types and origin. Linear approximation leads in case of aortas and bones to simple Voight's model. Modules of elasticity (in tensile loading) of aortas were from 102 kPa to 103 kPa. Module of elasticity of bones were from 106 Pa to 1010 Pa. Viscous coefficients of aortas were from 102 Pa.s to 103 Pa.s. Viscous coefficients of bones were from 100 Pa.s to 102 Pa.s. Nonlinearities: We found that following types of nonlinearities are significant: strain-stress relationship, time-dependent changes in elastic as well as viscose bodies. Strain and stress is well approximated by quadratic function σ = a ε2 + b ε + c with parameters a = 1833, b = 135, c = 20.0 (porcine aorta). Time-dependence in elastic coefficient: At the beginning of responses the elastic coefficient was of 42% lower then at 0.02 s of duration of the response (porcine aortas). Analogical results follow also from experiments on other structures (skin, bones)

Reliable reference gene selection for quantitative real time PCR in Haemonchus contortus

The aim of this work was to identify reliable reference genes for expression studies in adult Haemonchus contortus. Eleven candidate genes were identified and the stability of their expression was assessed in adult males and females of two genetically divergent H. contortus isolates: drug-susceptible (ISE) and multi-drug-resistant (WR). Five genes with the most stable expression patterns were further assessed for suitability as reference genes in anthelmintic-treated H. contortus adults versus non-treated controls. We identified important differences in the expression of a number of candidate genes in anthelmintic-treated samples, confirming the need for careful validation of control genes for such experiments. We propose the use of multiple reference genes for expression studies in this species and found gpd, ama and far most suitable for adult H. contortus

Metabolism of albendazole, ricobendazole and flubendazole in Haemonchus contortus adults: Sex differences, resistance-related differences and the identification of new metabolites

Haemonchus contortus (family Trichostrongylidae, Nematoda), a hematophagous gastrointestinal parasite found in small ruminants, has a great ability to develop resistance to anthelmintic drugs. We studied the biotransformation of the three benzimidazole anthelmintics: albendazole (ABZ), ricobendazole (albendazole S-oxide; RCB) and flubendazole (FLU) in females and males of H. contortus in both a susceptible ISE strain and resistant IRE strain. The ex vivo cultivation of living nematodes in culture medium with or without the anthelmintics was used. Ultrasensitive UHPLC/MS/MS analysis revealed 9, 7 and 12 metabolites of ABZ, RCB and FLU, respectively, with most of these metabolites now described in the present study for the first time in H. contortus. The structure of certain metabolites shows the presence of biotransformation reactions not previously reported in nematodes. There were significant qualitative and semi-quantitative differences in the metabolites formed by male and female worms. In most cases, females metabolized drugs more extensively than males. Adults of the IRE strain were able to form many more metabolites of all the drugs than adults of the ISE strain. Some metabolites were even found only in adults of the IRE strain. These findings suggest that increased drug metabolism may play a role in resistance to benzimidazole drugs in H. contortus. Keywords: Drug resistance, Drug metabolism, Anthelmintics, Benzimidazole, Nematod