Grapevine cvs Primitivo, Zinfandel and Crljenak kastelanski: Molecular analysis by AFLP

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Influence of distance from the host on parasitisation by Psyttalia concolor (Szépligeti) (Hymenoptera: Braconidae)

The olive fruit fly, Bactrocera oleae, is considered the main olive pest worldwide, and has been the target of biological control programmes through the release of the braconid parasitoid Psyttalia concolor. Laboratory tests were performed to evaluate the influence of distance from the host on parasitisation, placing larvae of the substitute host Ceratitis capitata at seven distances (0, 0.5, 1, 1.5, 2, 2.5, 3 mm) and four different time periods (7, 15, 30, 60 min). Moreover, field collected olives of Ogliarola Barese cultivar infested by B. oleae were exposed to P. concolor females to confirm its ability to parasitise B. oleae in small olives. Psyttalia concolor oviposition was inhibited at 2.5 and 3 mm due to the ovipositor length of the parasitoid females (2.7 mm). Hosts were easily parasitised at distances between 0 and 1.5 mm. The thin fruit pulp (up to 3.5 mm) of field collected olives allowed the parasitisation to occur also in mature fruits. At the best combination distance/time (0 mm, 30 min), tests performed with different larvae/parasitoid female ratio showed an increasing emergence of P. concolor (from 20% to 57%) with larvae/parasitoid ratio increasing from 0.11 to 0.74. The results of the present study might optimise the mass rearing of P. concolor, through a proper setting of its parameters, such as the host/parasitoid ratio, exposure distances, and interaction time

The effect of distance between parasitoid and host on the parasitism of Psyttalia concolor (Szépligeti) in organic olive orchards

Bactrocera oleae (Rossi) is a major pest of olive fruits. Biological control of olive fruit fly mainly relies on the activity of the Braconid species Psyttalia concolor (Szépligeti), which parasitizes Bactrocera oleae at the larval stage. Parasitism tests have been carried out at the insect growing facility (insectariums) of the Mediterranean Agronomic Institute of Bari (C.I.H.E.A.M.). The critical point of the use of P. concolor as a biological control agent in organic olive crop is the assessment of its effectiveness against B. oleae. Its lower presence in fruits of cultivated olives rather than in small fruits of wild olives suggests an influence of the size of the fruit, and of the thickness of its pulp on the parasitization of P. concolor. Therefore, the aim of this research was to investigate the capacity of the females of P. concolor to lay their eggs into the target host according to the distance existing between the female and the host larva. In these tests, the substitution host Ceratitis capitata was used and parasitism of P. concolor was tested at different distances (0, 0.5, 1, 1.5, 2, 2.5 and 3 mm) to the host larvae at different times (7 min, 15 min, 30 min and 60 min) of interaction. Predetermined distances between the parasitoids and the target larvae were arranged by locating the flying females into cubical cages of 40x40x40 cm with Plexiglas walls and the open roof covered with a tulle tissue in order to avoid that any insects from outside may enter the cages, and to allow the females to protrude their ovipositors. On these roofs, plastic cylinders containing the target larvae were placed with the open bases arranged with a tulle tissue, to allow the contact with the parasitoid females. Considering the width of the tissues negligible, distances were set by metal separators with increasing thickness. Parasitism level at 7 min was not significantly different at 2, 2.5 and 3 mm distances. Parasitism level was the highest at 0, 0.5, and 1 mm distances with no significant changes. Parasitism at 15 min showed a high variability according to the distances. The highest level resulted at 0.5 and 1 mm distances, the lowest at 3 mm distance. At 30 min interaction, the highest level of parasitism was at 0 mm distance, whilst at 1 and 1.5 mm the level was lower with no significant changes. Again, parasitism level was the lowest at 3 mm distance. The highest level of parasitism at 60 min was detected at 1.5 mm distance and was as lower as the distance increased. In conclusion, the study showed that the females of P. concolor were not able to parasite the target larvae at distances higher than 2.5 mm. Generally, the parasitism level decreases with the increase of the distance between target larvae and parasitoid females. At the lowest distances (0, 0.5 and 1 mm) hyper parasitism occurred and the target pupae were killed, but also the development of the parasitoids into adults was very difficult for nutrient unavailability. Biological control of P. concolor against B. oleae larvae may give satisfactory results only if the depth at which the target larvae are located into the olive drupes is not higher than 2 or 2.5 mm from the surfac