Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors

Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA. Aim: To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells. Methods: PtCl₄ (dbtp)₂ and PtCl₂ (6mp)₂ complexes were prepared. PtCl₄ (dbtp)₂ was created as modification of PtCl₄ (dmtp) test previously.Apoptosis and necrosis were examined using flow cytometry, upon Annexin V/PI staining. Results: LC₁₀,LC₅₀ andLC₉₀ parameters established for PtCl₄ (dbtp)₂ were as following: 2.6, 17.0, 58.0 μmol/L. However LC₁₀ andLC₅₀ established for PtCl₂ (6mp)₂ were 1.2 and 14.0μmol/l respectively. The both complexes induced apoptosis. PtCl₂ (6mp)₂ induced cell cycle arrest in G0/G1, while PtCl₄ (dbtp)₂ — in S-phase. Conclusions: PtCl₄ (dbtp)₂ appeared to be more cytotoxic against B16 cells than PtCl₂ (6mp)₂ . Apoptosis was the main mechanism of cell loss in cultures incubated with both tested complexes

Cis-Diiodido(N,N,N′,N′-tetra-methyl-ethylenediamine-k 2 N,N′)platinum(II)

In the title complex, cis-[PtI2(C6H16N2)], the Pt atom lies on a crystallographic twofold axis and the mol-ecule exhibits C 2 symmetry. The Pt atom is four-coordinated by a chelating N,N,N′,N′-tetra-methyl- ethylenediamine ligand [Pt - N = 2.081 (9) Å] and two iodide ligands [Pt - I = 2.589 (2) Å], forming a distorted square-planar geometry. © International Union of Crystallography 2007.Articl

New Dinuclear Macrocyclic Copper II Complexes as Potentially Fluorescent and Magnetic Materials

Two dinuclear copper II complexes with macrocyclic Schiff bases K1 and K2 were prepared by the template reaction of R 1,1 amp; 8242; binaphthalene 2,2 amp; 8242; diamine and 2 hydroxy 5 methyl 1,3 benzenedicarboxaldehyde K1, or 4 tert butyl 2,6 diformylphenol K2 with copper II chloride dihydrate. The compounds were characterized by spectroscopic methods. X ray crystal structure determination and DFT calculations confirmed their geometry in solution and in the solid phase. Moreover, intermolecular interactions in the crystal structure of K2 were analyzed using 3D Hirshfeld surfaces and the related 2D fingerprint plots. The magnetic study revealed very strong antiferromagnetic CuII CuII exchange interactions, which were supported by magneto structural correlation and DFT calculations conducted within a broken symmetry BS framework. Complexes K1 and K2 exhibited luminescent properties that may be of great importance in the search for new OLEDs. Both K1 and K2 complexes showed emissions in the range of 392 424 nm in solutions at various polarities. Thin materials of the studied compounds were deposited on Si 111 by the spin coating method or by thermal vapor deposition and studied by scanning electron microscopy SEM EDS , atomic force microscopy AFM , and fluorescence spectroscopy. The thermally deposited K1 and K2 materials showed high fluorescence intensity in the range of 318 531 nm for K1 Si and 326 472 nm for the K2 Si material, indicating that they could be used in optical device