Immunoaffinity isolation of Na,K-ATPase α3 isoform from pig kidney

AbstractThe Na,K-ATPase α3 isoform of the catalytic subunit has been isolated from pig kidney microsomes. The procedure employs immunoaffinity chromatography on Sepharose 4B covalently coupled with monospecific antibodies a-II against the synthetic peptide including the putative α3 N-terminus. The structural analysis provides unambiguous proof that the isolated protein corresponds to the third transcript for the α3 isoform. The N-terminal amino acid sequence determined, Met-Gly-Asp-Lys-Lys-Asp-Asp, shows that unlike the α1 and α2 proteins, the mature Na,K-ATPase α3 isoform lacks post-translational proteolytic processing.ATPase isozyme, (Na+ + K+)-; Immunoaffinity chromatography; N-terminal sequence analysi

Alignment problems of teaching in high school students with traffic active in student teams UrFU

Анализируются результаты пилотного исследования студентов УрФУ, разных курсов очного обучения по проблемам совмещения обучения с активной деятельностью в движении студенческих отрядов УрФУ, а также их удовлетворенности обучением в вузе. Данное исследование было проведено в декабре 2014 г. с целью написания курсовой работы.The results of the pilot study students UrFU different courses full-time study on the problems of combining active learning activities within the movement UrFU student teams, as well as their satisfaction with training in high school . This study was conducted in December 2014 with the aim of writing a term paper

CONTENT MARKETING AS A METHOD OF PROMOTION ONINSTAGRAM ON THE EXAMPLE OF LLC «INTERMEBEL»

В статье описаны основные тенденции продвижения в сети Интернет. Рассмотрена стратегия и особенности продвижения компаний мебельного сегмента в интернет-пространстве. Проведен анализ целевой аудитории ООО «Интермебель», изучен контент Instagram-аккаунта компании и ее конкурентов с целью разработки более эффективного алгоритма продвижения аккаунта ООО «Интермебель».This article discusses the features of the promotion of furniture segment companies in the Internet space. In this article were described the main trends of promotion on the Internet, the analysis of the target audience of LLC «Intermebel» was carried out, and the content of the Instagram account of the company and its competitors was considered to develop solutions for more eff ective promotion of the account of LLC «Intermebel»

Monitoring of invasions of family Percidae in the Volga-Caspian fishery basin

This paper presents long-term data on the occurrence of parasites of commercially valuable fish species, the common zander (Sander lucioperca (Linnaeus, 1758)) and the perch (Perca fluviatilis Linnaeus, 1758). The parasitofauna of perch fish was characterized by low species diversity, dominated by Nematoda. The component parasitic community was formed of host specific and euryxenic species with direct and complex development cycles. Species of epizootic and/or epidemiological significance common to perch and zander included Apophallus donicus Skrjabin & Lindtrop, 1919, Anisakis schupakovi Mosgovoi, 1951; Eustrongylides excisus Jägerskiöld, 1909; Corynosoma strumosum Rudolphi, 1802; Achteres percarum Nordmann, 1932; Argulus sp., and Piscicola geometra Linnaeus, 1761. The dynamics of infection of fish with most parasites showed seasonal variability. The larvae of the nematode E. excisus and the crustacean A. percarum caused a pathological response in the body of their host, whereas other species were asymptomatic parasites. The changes revealed in the occurrence and intensity of infection are due to the biological characteristics of the parasites and their hosts, as well as the means of transmission of the invasion. The annual presence and low levels of parasite infestation suggest stability of parasite-host relationships in perch fish. However, the presence of pathogens of invasive diseases indicates the maintenance of natural foci of parasitosis in the Volga-Caspian fishery subarea. Parasitic diseases being at the subclinical level suggest that the parasitological state of perch fish is satisfactory

Police misconduct in Russia: history and contemporaneity

The present paper focuses on the analysis of issues that arise in terms of theory and law enforcement practice. The key approach to the study of this problem is based on the distinctive difference between administrative and criminal offenses. The paper deals with the issues connected with the preventative activity of law-enforcement authorities of the Russian Federation. It reviews the doctrinal approach to the topic under consideration. The paper appeals to executors of law, students, and candidates for a master’s degree and postgraduates in the field of study “Jurisprudence”El presente documento se centra en el análisis de cuestiones que surgen en términos de teoría y práctica de aplicación de la ley. El enfoque clave para el estudio de este problema se basa en la diferencia distintiva entre los delitos administrativos y penales. El documento trata los problemas relacionados con la actividad preventiva de las autoridades encargadas de hacer cumplir la ley de la Federación Rusa. Revisa el enfoque doctrinal del tema en consideración. El artículo apela a los ejecutores de las leyes, estudiantes y candidatos para una maestría y postgrado en el campo de estudio "Jurisprudencia"

Study of Cognitive Disturbances by Neuropsychological Scales in Elderly

The article presents the results of a neuropsychological examination of 24 subjects aged 65–70 years. After analyzing the frequency of occurrence of the severity of cognitive impairment among men and women, it was revealed that there were no statistically significant differences on this basis.В статье представлены результаты нейропсихологического обследования 24 испытуемых возрастом 65–70 лет. Проанализировав частоту встречаемости степени выраженности когнитивных нарушений среди мужчин и женщин, не выявлены статистически значимые различия по данному признаку

Induction of antimeningitis immunity by synthetic peptides. III. Immunoactive synthetic fragments of the NspA protein from Neisseria meningitidis

Four potentially immunoactive peptide fragments of the NspA protein from the outer membrane of the bacterium Neisseria meningitidis were synthesized in order to create a synthetic vaccine against the meningococcal infection by the serogroup B bacterium. Mice of various lines were immunized with the free peptides nonconjugated with a protein carrier. All the synthetic peptides were shown to induce the production of the antipeptide antibodies in mice. A peptide capable of inducing a decrease in the number of bacteria in blood and the protection of infected animals from death was found in the experiments on the protection of the animals infected with two strains of the Neisseria meningitidis serogroup B

Synthesis and biological properties of polysaccharide-peptide conjugates as potential antigens for a vaccine against meningococci of serogroups A and B

A new approach to the development of a vaccine against meningococci of serogroups A and B was proposed. It involves the synthesis of conjugates of high-molecular capsule polysaccharides of the serogroup A meningococcus (PsA) with earlier synthesized protective fragments of membrane proteins from serogroup B meningococci. The conjugates were synthesized using a method that consists of the generation of aldehyde groups by oxidizing free vicinal hydroxyl groups of PsA and subsequent reaction of these groups with amino groups of the peptide. The reaction proceeds with the intermediate formation of the Schiff base, which is reduced to the stable secondary amine. The main parameters of the reaction were optimized in the synthesis of a PsA conjugate with a model peptide and methods of their characterization were developed. The reproducibility and efficiency of the synthetic procedure were demonstrated by the example of synthesis of PsA conjugates with fragments of protein PorA from the outer membrane of the serogroup B meningococcus. It was shown that, when administered without adjuvant, a conjugate of PsA with a protective peptide, which represents an exposed conserved fragment 306-332 of protein PorA, stimulates the formation of antibodies to the peptide and polysaccharide moieties of the molecule and is also capable of decreasing the degree of bacteremia in animals infected with serogroup A and serogroup B meningococci. The approach can be applied to the development of a complex vaccine for serogroup A and serogroup B meningococci. © 2008 MAIK Nauka

Induction of antimeningitis immunity by synthetic peptides: III. Immunoactive synthetic fragments of the NspA protein from Neisseria meningitidis

Four potentially immunoactive peptide fragments of the NspA protein from the outer membrane of the Neisseria meningitidis bacterium were synthesized in order to create a synthetic vaccine against meningococcal infection by the serogroup B bacterium. Mice of various lines were immunized with free peptides nonconjugated with a protein carrier. All the synthetic peptides were shown to induce the production of the antipeptide antibodies in mice. A peptide capable of inducing a decrease in the number of bacteria in blood and the protection of infected animals from death was found in the experiments on the protection of the animals infected with two strains of the Neisseria meningitidis serogroup B