Inhibition of gap junction and adherens junction assembly by connexin and A-CAM antibodies

We examined the roles of the extracellular domains of a gap junction protein and a cell adhesion molecule in gap junction and adherens junction formation by altering cell interactions with antibody Fab fragments. Using immunoblotting and immunocytochemistry we demonstrated that Novikoff cells contained the gap junction protein, connexin43 (Cx43), and the cell adhesion molecule, A-CAM (N-cadherin). Cells were dissociated in EDTA, allowed to recover, and reaggregated for 60 min in media containing Fab fragments prepared from a number of antibodies. We observed no cell-cell dye transfer 4 min after microinjection in 90% of the cell pairs treated with Fab fragments of antibodies for the first or second extracellular domain of Cx43, the second extracellular domain of connexin32 (Cx32) or A-CAM. Cell-cell dye transfer was detected within 30 s in cell pairs treated with control Fab fragments (pre-immune serum, antibodies to the rat major histocompatibility complex or the amino or carboxyl termii of Cx43). We observed no gap junctions by freeze-fracture EM and no adherens junctions by thin section EM between cells treated with the Fab fragments that blocked cell-cell dye transfer. Gap junctions were found on approximately 50% of the cells in control samples using freeze-fracture EM. We demonstrated with reaggregated Novikoff cells that: (a) functional interactions of the extracellular domains of the connexins were necessary for the formation of gap junction channels; (b) cell interactions mediated by A-CAM were required for gap junction assembly; and (c) Fab fragments of antibodies for A-CAM or connexin extracellular domains blocked adherens junction formation

An examination of try scoring in rugby union: a review of international rugby statistics

The purpose of this study was to review some of the key statistics collected by the International Rugby Board (IRB) in their review of the game (2003) and to compare these findings in relation to previous statistical analysis studies. Primary objectives of Rugby Union where identified using the IRB statement of intent. Statistics collected by the IRB for these objectives were then compared to parallel research in other sports regarding recommendations for effective play. The IRB statistics for Rugby Union would appear to replicate previously discovered characteristics of football (Reep and Benjamin, 1968), and would seem to favour a 'long ball' style of play in order to maximise scoring success. This paper highlights the IRB findings and illustrates the possible benefits of comparison and cross referencing with previous research in other sports

An examination of try scoring in rugby union: a review of international rugby statistics

The purpose of this study was to review some of the key statistics collected by the International Rugby Board (IRB) in their review of the game (2003) and to compare these findings in relation to previous statistical analysis studies. Primary objectives of Rugby Union where identified using the IRB statement of intent. Statistics collected by the IRB for these objectives were then compared to parallel research in other sports regarding recommendations for effective play. The IRB statistics for Rugby Union would appear to replicate previously discovered characteristics of football (Reep and Benjamin, 1968), and would seem to favour a 'long ball' style of play in order to maximise scoring success. This paper highlights the IRB findings and illustrates the possible benefits of comparison and cross referencing with previous research in other sports

The relationship between imaging-based body composition analysis and the systemic inflammatory response in patients with cancer: a systematic review

Background and aim: Cancer is the second leading cause of death globally. Nutritional status (cachexia) and systemic inflammation play a significant role in predicting cancer outcome. The aim of the present review was to examine the relationship between imaging-based body composition and systemic inflammation in patients with cancer. Methods: MEDLINE, EMBASE, Cochrane Library and Google Scholar were searched up to 31 March 2019 for published articles using MESH terms cancer, body composition, systemic inflammation, Dual energy X-ray absorptiometry (DEXA), magnetic resonance imaging (MRI), ultrasound sonography (USS) and computed tomography (CT). Studies performed in adult patients with cancer describing the relationship between imaging-based body composition and measures of the systemic inflammatory response were included in this review. Results: The literature search retrieved 807 studies and 23 met the final eligibility criteria and consisted of prospective and retrospective cohort studies comprising 11,474 patients. CT was the most common imaging modality used (20 studies) and primary operable (16 studies) and colorectal cancer (10 studies) were the most commonly studied cancers. Low skeletal muscle index (SMI) and systemic inflammation were consistently associated; both had a prognostic value and this relationship between low SMI and systemic inflammation was confirmed in four longitudinal studies. There was also evidence that skeletal muscle density (SMD) and systemic inflammation were associated (9 studies). Discussion: The majority of studies examining the relationship between CT based body composition and systemic inflammation were in primary operable diseases and in patients with colorectal cancer. These studies showed that there was a consistent association between low skeletal muscle mass and the presence of a systemic inflammatory response. These findings have important implications for the definition of cancer cachexia and its treatment

Giving Voice to Our Lakota People

Researchers at two universities worked with project partners to develop indigenous interpretive curriculum that provided the tools for indigenous students to tell their own stories and market their own heritage to visitors seeking an authentic experience. Project partners sought to identify elements that exemplify a culturally grounded approach to indigenous interpretation. It is assumed that indigenous interpretation will provide opportunities for non-native visitors to have meaningful experiences of native culture. It is also assumed that indigenous interpretation will foster a deeper understanding of the sacredness of native homelands, the resiliency and vulnerability of natural and cultural systems, and the beauty of harmonious relations between the earth and its inhabitants. Finally, it is assumed that as native youth gain skills in indigenous interpretation, they will make better decisions about which cultural stories and practices to share with the public (and which to retain unto themselves) and how to best share those stories with diverse audiences. This led to the development of indigenous interpretation and tourism curriculum in a tribal college in South Dakota. The proposed new degree plan includes core courses in natural and cultural history, psychology, business, Lakota language, and English. Within the set of core courses there is an emphasis in oral and written communication, digital communication, and experiential and place-based education. The project team proposed a set of three new interpretation courses and one new hospitality and tourism course to comprised the new concentration. The proposed course content and teaching pedagogy reflects an understanding of indigenous learning preferences and native ways of knowing. Proposed course content and teaching pedagogy also reflects professional standards of practice. Through a robust partnership, and active engagement by all partners in the curriculum development process, it is believed that indigenous students will gain new knowledge, an expanded skill set, and an enhanced career pathway. It is also believed that this partnership will provide opportunities to strengthen indigenous interpretation nationwide. Along with the new proposed concentration, the project team worked in tandem with fellow academics at tribal college to design program learning outcomes, assessment matrices and individual course syllabi. The four new courses proposed for the concentration will rely on adjunct faculty for course delivery that may include both an online and face to face component. Potential subject matter experts (SMEs) could include NPS personnel, hospitality and tourism professionals and others who bridge the gap between academia and practice. The model for this approach is based on a Cloud of Support that includes not only the Tribal Colleges and Universities {TCU), accrediting agency, faculty, staff and students, but also the Tribal Council, Elders, National Park Service (NPS), local partners and academic partners at other institutions

Jaw kinematics and mandibular morphology in humans

Abstract Understanding the influence of feeding behavior on mandibular morphology is necessary for interpreting dietary change in fossil hominins. However, mandibular morphology is also likely to have an effect on feeding behavior, including jaw kinematics. Here we examine the relationships between mandibular morphology and jaw kinematics in humans using landmark-based morphometrics to quantify jaw movement. Three-dimensional movements of reflective markers coupled to the mandible and cranium were used to capture jaw movements while subjects chewed cubes of raw and cooked sweet potato. Geometric morphometric methods were adapted to quantify and analyze gape cycle motion paths. Gape cycles varied significantly across chewing sequences and between raw and cooked sweet potato. Variation in gape cycle size and shape is related to the width (intergonial distance) and length of the mandible. These results underline the fact that jaw kinematic variation within and between taxa is related to and may be influenced by mandibular morphology. Future studies examining kinematic variation should assess the influence of morphological differences on movement

A comparison of CT-body composition measurements in non-cancer and cancer patients from a single UK centre

Objectives: Establish the prevalence of low skeletal muscle index and density in our population, by comparing age and sex matched cohorts of patients with and without cancer, using standardized methodology for CT-Body composition (CT-BC). Methods: A retrospective analysis of prospectively collected data. Patients admitted to our institution between 17th March 2020 - 1st May 2020, with confirmed coronavirus disease and imaging suitable for CT-BC (n=52), were age and sex matched with patients undergoing resection for colorectal cancer (n=52). Results: 104 patients were included in the final analysis. 43% (n=45) were male, 77% (n=80) were aged 65 years or older, 50% (n=50) were overweight (BMI ≥25) and 53% (n=55) were systemically inflamed (mGPS ≥1). The prevalence of a low SMI (56% vs. 65%) and low SMD (83% vs. 67%) was similar between cohorts. A low SMI and SMD were both associated with age (p<0.05 and p<0.01, respectively) on univariate analysis. On multivariate analysis, a low SMD was independently associated with age (OR 2.38 (1.34-4.22), p=0.003) and mGPS (OR 2.10 (1.20-3.68), p=0.01). Conclusions: In conclusion, the prevalence of a low SMI and low SMD was similar in non-cancer and cancer cohorts in our institution

Comparison of the prognostic value of ECOG-PS, MGPS and BMI/WL: Implications for a clinically important framework in the assessment and treatment of advanced cancer

BACKGROUND AND AIMS:The systemic inflammatory response is associated with the loss of lean tissue, anorexia, weakness, fatigue and reduced survival in patients with advanced cancer and therefore is important in the definition of cancer cachexia. The aim of the present study was to carry out a direct comparison of the prognostic value of Eastern Cooperative Oncology Group Performance Status (ECOG-PS), modified Glasgow Prognostic Score (mGPS) and Body Mass Index/Weight Loss Grade (BMI/WL grade) in patients with advanced cancer. METHOD:All data were collected prospectively across 18 sites in the UK and Ireland. Patient's age, sex, ECOG-PS, mGPS and BMI/WL grade were recorded, as were details of underlying disease including metastases. Survival data were analysed using univariate and multivariate Cox regression. RESULTS:A total of 730 patients were assessed. The majority of patients were male (53%), over 65 years of age (56%), had an ECOG-PS>0/1 (56%), mGPS≥1 (56%), BMI≥25 (51%), <2.5% weight loss (57%) and had metastatic disease (86%). On multivariate cox regression analysis ECOG-PS (HR 1.61 95%CI 1.42-1.83, p < 0.001), mGPS (HR 1.53, 95%CI 1.39-1.69, p < 0.001) and BMI/WL grade (HR 1.41, 95%CI 1.25-1.60, p < 0.001) remained independently associated with overall survival. In patients with a BMI/WL grade 0/1 both ECOG and mGPS remained independently associated with overall survival. CONCLUSION:The ECOG/mGPS framework may form the basis of risk stratification of survival in patients with advanced cancer