Identification of new early markers of the hypersensitive response in Arabidopsis thaliana11The Genbank accession numbers for the sequences reported in this paper are AJ131391 (Athsr2), AJ131392 (Athsr3),AJ243377 (Athsr4), AJ243378 (Athsr5), AJ243379 (Athsr6) and AJ243380 (Athsr7).

AbstractNew molecular markers of the hypersensitive response (HR) of Arabidopsis thaliana to the bacterial pathogen Xanthomonas campestris pv. campestris (X.c.c..) have been identified by differential screening of a cDNA library constructed from suspension cells inoculated by an HR-inducing strain in the presence of cycloheximide. Seven families of genes (called Athsr) have been isolated, show similarities to voltage-dependent anion channels (VDAC) and alternative oxidases, or are novel proteins. Athsr genes have shown to be specifically or preferentially expressed during the HR. These data suggest that Athsr genes might be involved in early events conditioning the establishment of the HR

A GRASPxELS with Depth First Search Split Procedure for the HVRP

Split procedures have been proved to be efficient within global framework optimization for routing problems by splitting giant tour into trips. This is done by generating optimal shortest path within an auxiliary graph built from the giant tour. An efficient application has been introduced for the first time by Lacomme et al. (2001) within a metaheuristic approach to solve the Capacitated Arc Routing Problem (CARP) and second for the Vehicle Routing Problem (VRP) by Prins (2004). In a further step, the Split procedure embedded in metaheuristics has been extended to address more complex routing problems thanks to a heuristic splitting of the giant tour using the generation of labels on the nodes of the auxiliary graph linked to resource management. Lately, Duhamel et al. (2010) defined a new Split family based on a depth first search approach during labels generation in graph. The efficiency of the new split method has been first evaluated in location routing problem with a GRASP metaheuristic. Duhamel et al. (2010) provided full numerical experiments on this topic

A GRASPxELS with Depth First Search Split Procedure for the HVRP

Split procedures have been proved to be efficient within global framework optimization for routing problems by splitting giant tour into trips. This is done by generating optimal shortest path within an auxiliary graph built from the giant tour. An efficient application has been introduced for the first time by Lacomme et al. (2001) within a metaheuristic approach to solve the Capacitated Arc Routing Problem (CARP) and second for the Vehicle Routing Problem (VRP) by Prins (2004). In a further step, the Split procedure embedded in metaheuristics has been extended to address more complex routing problems thanks to a heuristic splitting of the giant tour using the generation of labels on the nodes of the auxiliary graph linked to resource management. Lately, Duhamel et al. (2010) defined a new Split family based on a depth first search approach during labels generation in graph. The efficiency of the new split method has been first evaluated in location routing problem with a GRASP metaheuristic. Duhamel et al. (2010) provided full numerical experiments on this topic

A Framework for the Evaluation of Biosecurity, Commercial, Regulatory, and Scientific Impacts of Plant Viruses and Viroids Identified by NGS Technologies

Recent advances in high-throughput sequencing technologies and bioinformatics have generated huge new opportunities for discovering and diagnosing plant viruses and viroids. Plant virology has undoubtedly benefited from these new methodologies, but at the same time, faces now substantial bottlenecks, namely the biological characterization of the newly discovered viruses and the analysis of their impact at the biosecurity, commercial, regulatory, and scientific levels. This paper proposes a scaled and progressive scientific framework for efficient biological characterization and risk assessment when a previously known or a new plant virus is detected by next generation sequencing (NGS) technologies. Four case studies are also presented to illustrate the need for such a framework, and to discuss the scenarios.Peer reviewe

The TGB1 Movement Protein of Potato virus X Reorganizes Actin and Endomembranes into the X-Body, a Viral Replication Factory

Potato virus X (PVX) requires three virally encoded proteins, the triple gene block (TGB), for movement between cells. TGB1 is a multifunctional protein that suppresses host gene silencing and moves from cell to cell through plasmodesmata, while TGB2 and TGB3 are membrane-spanning proteins associated with endoplasmic reticulum-derived granular vesicles. Here, we show that TGB1 organizes the PVX “X-body,” a virally induced inclusion structure, by remodeling host actin and endomembranes (endoplasmic reticulum and Golgi). Within the X-body, TGB1 forms helically arranged aggregates surrounded by a reservoir of the recruited host endomembranes. The TGB2/3 proteins reside in granular vesicles within this reservoir, in the same region as nonencapsidated viral RNA, while encapsidated virions accumulate at the outer (cytoplasmic) face of the X-body, which comprises a highly organized virus “factory.” TGB1 is both necessary and sufficient to remodel host actin and endomembranes and to recruit TGB2/3 to the X-body, thus emerging as the central orchestrator of the X-body. Our results indicate that the actin/endomembrane-reorganizing properties of TGB1 function to compartmentalize the viral gene products of PVX infection

HF-IRP: heterogeneous fleet inventory routing problem

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