Vitis vinifera L. germplasm diversity: a genetic and ampelometric study in ancient vineyards in the South of Basilicata region (Italy)

The evaluation of the existing grapevines biodiversity in several areas still unexplored in Basilicata region has been carried out. A four years survey in ancient vineyards of Potenza was performed to investigate grapevine biodiversity. 85 collected accessions were subjected to genetic characterization through nine microsatellite markers. A total of 42 genotypes were obtained. The comparison with national and international databases allowed the identification of 26 accessions corresponded to new autochthonous genotypes and minor/local cultivars, in addition 16 international and national cultivars commonly cultivated in several Italian regions were found (data not shown in this work). Results indicated that minor/local cultivars were mainly cultivated in the near regions. The genetic profile of 9 new autochthonous grapevines was described here for the first time. Comparison of the genotypes, allelic frequencies, allelic sizes and ampelometric traits on mature leaves are highlighted. Conservation of new autochthonous and minor/local cultivars in germoplasm collections has been carried out including them in the germoplasm collection of CREA-VE in Arezzo in order to save grapevine biodiversity and allows further agronomical and enological evaluation

TUNNELING OPERATIONS, OCCUPATIONAL S&H AND ENVIRONMENTAL PROTECTION: A PREVENTION THROUGH DESIGN APPROACH

The study refers on the main results of a research work that has been carried out by the Safety and Health group of the Department of Environment, Land and Infrastructure engineering (DIATI) at the Politecnico di Torino, with reference to the substantial difference that exists between the case of an established absence of hazards due to the presence of noxious materials such asbestos, crystalline silica, in the rock mass to be excavated and the situations in which these minerals can be present. Since, when carcinogenic substances can be expected, corrective action following exposure or dispersion is clearly unacceptable, the presence of critical pollutants requires special preventive actions for the health of the workers and the muck which can introduce risks for the users (e.g., during mechanical processing involving feeding, crushing, milling, sizing and sorting operations) should not be considered for any reuse

Valutazione e gestione dei rischi per la salute dei lavoratori nello scavo meccanico di gallerie

La presente relazione costituisce il compendio di quanto discusso all’interno del seminario organizzato anche con valenza formativa nell’ambito del Master di 2° livello in Ingegneria per la Sicurezza Occupazionale -MISO- e accreditato Educazione Continua in Medicina -ECM-, dal titolo “Valutazione e gestione dei rischi per la salute dei lavoratori nello scavo meccanico di gallerie”. Nei vari interventi, dopo un inquadramento sulle moderne tecniche di scavo meccanico, sono trattati i criteri di approccio per la valutazione e gestione dei principali inquinanti di formazione o generati durante la lavorazione. Sono in particolare approfonditi gli aspetti connessi con la definizione di valori di concentrazione accettabili con uno scenario normativo tuttora in marcata evoluzione. L’ultima parte fornisce indicazioni sulla gestione della sicurezza in coerenza con il moderno approccio in Prevention through Design -PtD- e in qualità, in condizioni normali ed in caso di deviazioni ragionevolmente attendibili. Nel suo insieme quindi, quanto esposto può costituire riferimento aggiornato sullo stato dell’arte in materia di sicurezza per lo scavo meccanico delle gallerie

occupational safety and health in tunnelling in rocks formations potentially containing asbestos good practices for risk assessment and management

In tunnelling operations, Occupational Safety and Health (OS&H) and Environmental Protection of the areas close to the tunnel portal become even more critical in case of rock formations potentially containing asbestos, quartz, radioactive elements, etc. In order to limit the workers' exposure and the environmental impact becomes in these cases of paramount importance to preliminarily analyze and quantify the possible presence of the pollutants in the rock and, if necessary, to implement suitable measures to avoid/minimize their emission from the winning and mucking operations. However, in case of asbestos minerals, to obtain reliable results from the preliminary analysis is a challenging task, due to the complex patterns of occurrence of asbestos within the host rock. Consequently, the definition of special monitoring, alarm and control systems is essential during the tunnel excavation. The paper summarizes the results of a thorough study aimed at defining the most suitable monitoring techniques in uncertain situations and the residual criticalities, essentially due to the delay between the beginning of the pollutant release at the tunnel face and its detection. The final part of the work deals with the possible innovative prevention solutions suitable to minimize the previously mentioned delay and ensure the safety of the workers along the time necessary to stop the activities and evacuate the tunnel, on hold of the activation of a special "asbestos" organization

A Prevention through Design Approach for the Environmental S&H Conditions and the Ventilation System at an Italian Underground Quarry

Even if the number of the Italian quarrying operations in underground is steadily growing, in many cases the safety criticalities are somehow underestimated, in spite of the regulations D.Lgs. 81/08 and D.Lgs. 624/96, Italian enforcements of the European Directives. Ventilation system is conceived to face very simplified requirements, whilst critical pollutants or emergency situations are not taken in due considerations. Asides, the ventilation system fault and availability analysis is seldom included in the project. The paper deals with the results of a research work started some years ago at an underground quarry exploited through drift sublevel based on drill and blast technique, to identify criteria suitable to grant effective safety and health -S&H- conditions for the workers operating in the underground in the Prevention through Design approach. Taken into account that the phases for an effective approach to the S&H problems in underground must follow a hierarchic method in which the risk management should be faced from an effective hazard reduction to a minimum at the sources, and the ventilation should be considered only as a 4th level solution, the possibilities of control at the main pollution sources, i.e. the emission of pollutants due to the rock winning and mucking operations, have been examined. The residual risk was then faced with both the original underground and airways layout definition for a new exploitation development, based on technical and efficiency considerations, and on fire emergency computer simulations. Finally, the paper summarizes the results of an availability analysis of the ventilation system for the normal operating conditions and the emergencies management, on the basis of the results of Hazard Evaluation techniques, in particular Hazard and Operability Analysis and Fault Tree Analysis

Occupational Risk Assessment and Management in a System Approach: the Why and the How

Occupational Safety and Health - OS&H problems still remain an important criticality in many Statistical classification of economic activities in the European Community - NACE sectors in Italy. Even if the official Italian statistical data published by National Institute for Work Injury Insurance - Inail show some fluctuations in the yearly accident and health impairment rates, the 3 fatalities/day figure proves difficult to modify, and the number of traditional and new professional diseases remains very impressive (officially approximately 2/year deadly diseases). An extensive and in deep analysis of a number of case histories, carried out by means of an innovative technique (namely the Computer aided Cause Consequence for Prevention - CCCP approach) conclusively proved that this unacceptable scenario results from the following causes: a - Hazard Factors (situations, materials, substances, organization, reasonably predictable faults and errors in terms of Worst Credible Case - WCC, etc.) poor Identification, directly involving the absence of special prevention countermeasures; b – Poor Risk Assessment, involving under or overestimation of the seriousness of some safety problems, and hence a misguided organization of the prevention; c – Poor Risk Management, basically in terms of lacking internal/external audits of situations/operations vs the Risk Assessment results, lacking risk assessment revision in case of scenery modifications, and lacking conservation along the time of the safety measures resulting from the risk assessment. A side cause of the modest success of the actions aimed to modify the previously mentioned concerning occupational safety scenery is that the Heinrich approach (prevention can be implemented from information on the frequency of deviations from a correct working situation) is applied in a generalized way without discussion. But the Heinrich model is based on a “Person Approach” (the misconduct of victims or colleagues is the main cause of deviation, in spite of the obvious consideration that they and they only are in direct contact with the Hazard Factor). A Guideline based on a System Approach (the system approach concentrates on the conditions under which individuals work and tries to build defences to avert errors or mitigate their effects) was developed and successfully tested in a number different situations. The basic principia of the guideline, coherent with both the 89/391 EEC Directive and the OS&H in quality criteria, can be summarized in a 3E list: E1 - Engineering: Prevention through Design – PtD, focused to guarantee the highest safety level technically achievable through comparative analysis of alternative solutions, and the possible faults, human errors included (a formal demonstration is necessary); E2 - Education: information, education and training actions at every hierarchic level, derived from a Risk Assessment and Management specially conceived for the situation under exam; E3 - Enforcement: checking with internal/external audits the preservation through time of the safety level made possible by the PtD approach, and consideration of further improvements made available by the technical and epidemiology improvements. The paper discusses the previous aspects, and the Why and the How of the suggested Occupational Risk Assessment and Management technique in a System Approach

A Prevention through Design Approach for the Environmental S&H Conditions and the Ventilation System at an Italian Underground Quarry

Even if the number of the Italian underground quarrying operations is steadily growing, in many cases the safety criticalities are somehow underestimated, in spite of the regulations D.Lgs. 81/08 and D.Lgs. 624/96, Italian enforcements of the former European Directives 89/391/EEC, 92/91/EEC and 92/104/EEC. Ventilation system is conceived to face very simplified requirements, whilst critical pollutants or emergency situations are not taken in due consideration. Asides, the ventilation system fault and availability analysis are seldom included in the project. The paper deals with the results of a research work started some years ago at an underground quarry exploited through drift sublevel based on drill and blast technique, to identify criteria suitable to grant effective safety and health -S&H- conditions for the workers operating in the underground in the Prevention through Design approach. Taken into account that the phases for an effective approach to the S&H problems in underground must follow a hierarchic method, in which the risk management should be faced from an effective hazard reduction to a minimum at the sources, and the ventilation should be considered only as a 4th level solution, the possibilities of control at the main pollution sources, i.e. the emission of pollutants due to the rock winning and mucking operations, have been examined. The residual risk was then faced with both the original underground and airways layout definition for a new exploitation development, based on technical and efficiency considerations, and on fire emergency computer simulations. Finally, the paper summarizes the results of an availability analysis of the ventilation system for normal operating conditions, and the emergencies management, on the basis of the results of Hazard Evaluation techniques, particularly Hazard and Operability Analysis and Fault Tree Analysis