Tenchini’s legacy as a starting point for the reconstruction of the history of a criminal in the 19th century|L’eredità del Tenchini come punto di partenza per la ricostruzione della storia di un criminale del XIX secolo

Late in the 19th century, physician Lorenzo Tenchini undertook a study of criminal subjects and psychiatric patients. One of these was B.A., a 22-year-old soldier who committed suicide after attempting to kill his lover, a prostitute named G.I. The starting points of this study were the analysis of the subject's biography – data which resulted from the autopsy – and several observations on his brain and skull in accordance with the phrenological doctrine of that time. The goal of this work was to shed new light on an Italian criminal of the late 19th century and on Tenchini's work, combining the material collected by the doctor himself with sources of the period and modern medical knowledge

Sub-Muscular Direct-to-Implant Immediate Breast Reconstruction in Previously Irradiated Patients Avoiding the Use of ADM: A Preliminary Study

Background: The aim of this paper is to present a preliminary experience of sub-muscular primary direct-to-implant (DTI) breast reconstruction without acellular dermal matrix (ADM), after salvage mastectomy for local recurrence following prior irradiation. Methods: A retrospective investigation was performed on a prospectively maintained database of breast reconstruction cases at our institution between January 2015 and December 2020. We considered only immediate DTI breast reconstructions without ADM following radiotherapy and salvage mastectomy for local recurrence, with at least a 12-month follow-up. Results: The study considered 18 female patients with an average of 68 years. According to the BREAST-Q questionnaire, all patients reported high levels of “satisfaction with outcome” with good “psychosocial wellness” and “physical impact” related to the reconstruction. The aesthetic evaluation showed a significant difference between the VAS score gave by the patient (mean 6.9) and the surgeon (mean 5.4). No implant exposure occurred in this series. In terms of complications, four patients (22%) suffered from wound dehiscence and were managed conservatively. Three patients (17%) required primary closure in day surgery following superficial mastectomy flap necrosis. Late capsular contracture was seen in seven patients (four Baker stage II and three Baker stage III, totally 39%); however, no patient was willing to undergo implant exchange. Conclusions: DTI breast reconstruction following prior irradiation can be considered as an option in patients who are not good candidates for autologous breast reconstruction. Our general outcomes compared favorably with literature data regarding the use of staged procedures, with acceptable complication rates and levels of patient satisfaction

Surgical treatment of pressure injuries in children: A multicentre experience

Pressure injuries (PI) are infrequent in paediatric patients, prevalence estimates ranging from 1.4% to 8.2%, and reaching values as high as 43.1% in critical care areas. They can be associated with congenital neurological or metabolic disorders that cause reduced mobility or require the need for medical devices. In children, most pressure injuries heal spontaneously. However, a small percentage of ulcers that is refractory to conservative management or is too severe at presentation (Stage 3 or 4) will be candidates for surgery. We retrospectively reviewed the clinical history of paediatric patients affected by pressure injuries from four European Plastic Surgery Centres. Information was collected from clinical and radiology records, and laboratory reports. An accurate search of the literature revealed only two articles reporting on the surgical treatment of pressure injuries in children. After debridement, we performed surgical coverage of the pressure injuries. We report here our experience with 18 children aged 1-17 years, affected by pressure injury Stages 3 and 4. They were successfully treated with pedicled (17 patients) or free flaps (1 patient). The injuries involved the sacrum (6/18 patients), lower limb (3/18 patients), thoracic spine (2/18 patients), ischium (3/18 patients, bilateral in one patient), temporal area (3/18 patients), hypogastrium (1/18 patients) and were associated to medical devices in three cases. Flaps were followed for a minimum of 19 months and up to 13 years. Only two patients developed true recurrences that were treated again surgically. Pressure injuries are infrequent in children and rarely need surgical treatment. Pedicled flaps have a high success rate. Recurrences, contrary to what is reported in the literature, were rare

Case Report: Could Hennebert's Sign Be Evoked Despite Global Vestibular Impairment on Video Head Impulse Test? Considerations Upon Pathomechanisms Underlying Pressure-Induced Nystagmus due to Labyrinthine Fistula

We describe a case series of labyrinthine fistula, characterized by Hennebert's sign (HS) elicited by tragal compression despite global hypofunction of semicircular canals (SCs) on a video-head impulse test (vHIT), and review the relevant literature. All three patients presented with different amounts of cochleo-vestibular loss, consistent with labyrinthitis likely induced by labyrinthine fistula due to different temporal bone pathologies (squamous cell carcinoma involving the external auditory canal in one case and middle ear cholesteatoma in two cases). Despite global hypofunction on vHIT proving impaired function for each SC for high accelerations, all patients developed pressure-induced nystagmus, presumably through spared and/or recovered activity for low-velocity canal afferents. In particular, two patients with isolated horizontal SC fistula developed HS with ipsilesional horizontal nystagmus due to resulting excitatory ampullopetal endolymphatic flows within horizontal canals. Conversely, the last patient with bony erosion involving all SCs developed mainly torsional nystagmus directed contralaterally due to additional inhibitory ampullopetal flows within vertical canals. Moreover, despite impaired measurements on vHIT, we found simultaneous direction-changing positional nystagmus likely due to a buoyancy mechanism within the affected horizontal canal in a case and benign paroxysmal positional vertigo involving the dehiscent posterior canal in another case. Based on our findings, we might suggest a functional dissociation between high (impaired) and low (spared/recovered) accelerations for SCs. Therefore, it could be hypothesized that HS in labyrinthine fistula might be due to the activation of regular ampullary fibers encoding low-velocity inputs, as pressure-induced nystagmus is perfectly aligned with the planes of dehiscent SCs in accordance with Ewald's laws, despite global vestibular impairment on vHIT. Moreover, we showed how pressure-induced nystagmus could present in a rare case of labyrinthine fistulas involving all canals simultaneously. Nevertheless, definite conclusions on the genesis of pressure-induced nystagmus in our patients are prevented due to the lack of objective measurements of both low-acceleration canal responses and otolith function

A three-component giant radio halo: the puzzling case of the galaxy cluster Abell 2142

Turbulence introduced into the intra-cluster medium (ICM) through cluster merger events transfers energy to non-thermal components, and can trigger the formation of diffuse synchrotron radio sources. Typical diffuse sources in the forms of giant radio halos and mini-halos are found in merging and relaxed cool core galaxy clusters, respectively. On the other hand, recent observations have revealed an increasing complexity of the non-thermal phenomenology. Abell 2142 (A2142) is a mildly disturbed cluster that exhibits uncommon thermal and non-thermal properties. It is known to host a hybrid halo consisting of two components (H1 and H2), namely a mini-halo-like and an enigmatic elongated radio halo-like structure. We aim to investigate the properties, origin, and connections of each component. We present deep LOFAR observations of A2142 in the frequency ranges $30-78$ MHz and $120-168$ MHz. With complementary multi-frequency radio and X-ray data, we analyse the radio spectral properties of the halo and assess the connection between the non-thermal and thermal components of the ICM. We detected a third radio component (H3), which extends over the cluster volume on scales $\sim 2$ Mpc, embeds H1 and H2, and has a morphology that roughly follows the thermal ICM distribution. The radio spectral index is moderately steep in H1 ($\alpha=1.09\pm 0.02$) and H2 ($\alpha=1.15\pm 0.02$), but is steeper ($\alpha=1.57\pm 0.20$) in H3. The analysis of the thermal and non-thermal properties allowed us to discuss possible formation scenarios for each radio component. Turbulence from sloshing motions of low-entropy gas on different scales may be responsible for the origin of H1 and H2. We classified H3 as a giant ultra-steep spectrum radio halo, which could trace the residual activity from an old energetic merger and/or inefficient turbulent re-acceleration induced by ongoing minor mergers.Comment: 24 pages (including Appendix), 15 Figures (plus 4 Figures in Appendix), Accepted for publication by A&

Abell 746: A highly disturbed cluster undergoing multiple mergers

We present deep \textit{XMM-Newton}, Karl Jansky Very Large Array, and upgraded Giant Metrewave Radio Telescope observations of Abell 746, a cluster that hosts a plethora of diffuse emission sources that provide evidence for the acceleration of relativistic particles. Our new \textit{XMM-Newton} images reveal a complex morphology of the thermal gas with several substructures. We observe an asymmetric temperature distribution across the cluster: the southern regions exhibit higher temperatures, reaching $\sim$9\,keV, while the northern regions have lower temperatures ($\rm \leq4\,keV$), likely due to a complex merger. We find evidence of four surface brightness edges, of which three are merger-driven shock fronts. Combining our new data with the published LOw-Frequency ARray observations has unveiled the nature of diffuse sources in this system. The bright northwest relic shows thin filaments and high degree of polarization with aligned magnetic field vectors. We detect a density jump, aligned with the fainter relic to the north. To the south, we detect high-temperature regions, consistent with shock-heated regions and density jump coincident with the northern tip of the southern radio structure. Its integrated spectrum shows a high-frequency steepening. Lastly, we find that the cluster hosts large-scale radio halo emission. The comparison of the thermal and nonthermal emission reveals an anticorrelation between the bright radio and X-ray features at the center. Our findings suggest that Abell 746 is a complex system that involves multiple mergers.Comment: 21 pages, 13 figures, submitted to Ap

The RAD51 and DMC1 homoeologous genes of bread wheat: cloning, molecular characterization and expression analysis

<p>Abstract</p> <p>Background</p> <p>Meiotic recombination in eukaryotes requires two homologues of the <it>E. coli </it>RecA proteins: Rad51 and Dmc1. Both proteins play important roles in the binding of single stranded DNA, homology search, strand invasion and strand exchange. Meiotic recombination has been well studied in Arabidopsis, rice, maize and the orthologues of <it>RAD51 </it>and <it>DMC1 </it>have been characterized. However genetic analysis of the <it>RAD51 </it>and <it>DMC1 </it>genes in bread wheat has been hampered due to the absence of complete sequence information and because of the existence of multiple copies of each gene in the hexaploid wheat genome.</p> <p>Findings</p> <p>In this study we have identified that <it>TaRAD51 </it>and <it>TaDMC1 </it>homoeologues are located on group 7 and group 5 chromosomes of hexaploid wheat, respectively. Comparative sequence analysis of cDNA derived from the <it>TaRAD51 </it>and <it>TaDMC1 </it>homoeologues revealed limited sequence divergence at both the nucleotide and the amino acid level. Indeed, comparisons between the predicted amino acid sequences of <it>TaRAD51 </it>and <it>TaDMC1 </it>and those of other eukaryotes reveal a high degree of evolutionary conservation. Despite the high degree of sequence conservation at the nucleotide level, genome-specific primers for cDNAs of <it>TaRAD51 </it>and <it>TaDMC1 </it>were developed to evaluate expression patterns of individual homoeologues during meiosis. QRT-PCR analysis showed that expression of the <it>TaRAD51 </it>and <it>TaDMC1 </it>cDNA homoeologues was largely restricted to meiotic tissue, with elevated levels observed during the stages of prophase I when meiotic recombination occurs. All three homoeologues of both strand-exchange proteins (<it>TaRAD51 </it>and <it>TaDMC1</it>) are expressed in wheat.</p> <p>Conclusions</p> <p>Bread wheat contains three expressed copies of each of the <it>TaRAD51 </it>and <it>TaDMC1 </it>homoeologues. While differences were detected between the three cDNA homoeologues of <it>TaRAD51 </it>as well as the three homoeologues of <it>TaDMC1</it>, it is unlikely that the predicted amino acid substitutions would have an effect on the protein structure, based on our three-dimensional structure prediction analyses. There are differences in the levels of expression of the three homoeologues of <it>TaRAD51 </it>and <it>TaDMC1 </it>as determined by QRT-PCR and if these differences are reflected at the protein level, bread wheat may be more dependent upon a particular homoeologue to achieve full fertility than all three equally.</p

Separation of Recombination and SOS Response in Escherichia coli RecA Suggests LexA Interaction Sites

RecA plays a key role in homologous recombination, the induction of the DNA damage response through LexA cleavage and the activity of error-prone polymerase in Escherichia coli. RecA interacts with multiple partners to achieve this pleiotropic role, but the structural location and sequence determinants involved in these multiple interactions remain mostly unknown. Here, in a first application to prokaryotes, Evolutionary Trace (ET) analysis identifies clusters of evolutionarily important surface amino acids involved in RecA functions. Some of these clusters match the known ATP binding, DNA binding, and RecA-RecA homo-dimerization sites, but others are novel. Mutation analysis at these sites disrupted either recombination or LexA cleavage. This highlights distinct functional sites specific for recombination and DNA damage response induction. Finally, our analysis reveals a composite site for LexA binding and cleavage, which is formed only on the active RecA filament. These new sites can provide new drug targets to modulate one or more RecA functions, with the potential to address the problem of evolution of antibiotic resistance at its root