BRONCHIOLITE MUCOIDE DIFFUSA IN CORSO DI ASMA BRONCHIALE

BRONCHIOLITE MUCOIDE DIFFUSA IN CORSO DI ASMA BRONCHIALE

Antibacterial Effect of Stainless Steel Surfaces Treated with a Nanotechnological Coating Approved for Food Contact

Stainless steel, widely present in the food industry, is frequently exposed to bacterial colonization with possible consequences on consumers’ health. 288 stainless steel disks with different roughness (0.25, 0.5 and 1 μm) were challenged with four Gram-negative (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 1402, Yersinia enterocolitica ATCC 9610 and Pseudomonas aeruginosa ATCC 27588) and four Gram-positive bacteria (Staphylococcus aureus ATCC 6538, Enterococcus faecalis ATCC 29212, Bacillus cereus ATCC 14579 and Listeria monocytogenes NCTT 10888) and underwent three different sanitizing treatments (UVC, alcohol 70% v/v and Gold lotion). Moreover, the same procedure was carried out onto the same surfaces after a nanotechnological surface coating (nanoXHAM® D). A significant bactericidal effect was exerted by all of the sanitizing treatments against all bacterial strains regardless of roughness and surface coating. The nanoXHAM® D coating itself induced an overall bactericidal effect as well as in synergy with all sanitizing treatments regardless of roughness. Stainless steel surface roughness is poorly correlated with bacterial adhesion and only sanitizing treatments can exert significant bactericidal effects. Most of sanitizing treatments are toxic and corrosive causing the onset of crevices that are able to facilitate bacterial nesting and growth. This nanotechnological coating can reduce surface adhesion with consequent reduction of bacterial adhesion, nesting, and growth

Arresto cardiocircolatorio nel territorio di modena e provincia: uno studio retrospettivo osservazionale

Cardiac arrest is a sudden stop in effective blood circulation due to failure of the heart to contract effectively or at all. The present study analyses cardiac arrest in Modena during year 2012. 118 has assisted 1142 people in cardiac arrest. 652 patients were in cardiac arrest for a cardiac reason, 172 for trauma, 85 for respiratory reasons, 21 for drugs, 34 for neurologic reasons, 104 for tumoral reasons, 33 for bleeding. In 355 cases cardiac resuscitation was made. 230 were male and 125 female. The median age was 73,8 years old. For 255 patients cardiac arrest happened at home The return to a normal circulation happened in 85 people. 36 patients survived and were discharged from hospital. For the future it is necessary to spread to population basic life support and BLSD to improve the outcome

Time-course study of the antibacterial activity of an amorphous SiOxCyHz coating certified for Food Contact

One of the most-used food contact materials is stainless steel (AISI 304L or AISI 316L), owing to its high mechanical strength, cleanability, and corrosion resistance. However, due to the presence of minimal crevices, stainless-steel is subject to microbial contamination with consequent significant reverb on health and industry costs due to the lack of effective reliability of sanitizing agents and procedures. In this study, we evaluated the noncytotoxic effect of an amorphous SiOxCyHz coating deposited on stainless-steel disks and performed a time-course evaluation for four Gram-negative bacteria and four Gram-positive bacteria. A low cytotoxicity of the SiOxCyHz coating was observed; moreover, except for some samples, a five-logarithm decrease was visible after 1 h on coated surfaces without any sanitizing treatment and inoculated with Gram-negative and Grampositive bacteria. Conversely, a complete bacterial removal was observed after 30 s – 1 min application of alcohol and already after 15 s under UVC irradiation against both bacterial groups. Moreover, coating deposition changed the wetting behaviors of treated samples, with contact angles increasing from 90.25° to 113.73°, realizing a transformation from hydrophilicity to hydrophobicity, with tremendous repercussions in various technological applications, including the food industry

A Time-Course Study on a Food Contact Material (FCM)-Certified Coating Based on Titanium Oxide Deposited onto Aluminum

Aluminum is the second most widely used metal worldwide. It is present as an additive in cosmetics, pharmaceuticals, food, and food contact materials (FCM). In this study, we confirm the bactericidal effect of a special anodizing method, based on TiO2 nanoparticles (DURALTI®) deposited on aluminum disks with different roughness and subjected to two sanitizing treatments: UV and alcohol 70%. Consequently, we perform a time-course evaluation against both Gram-negative and Gram-positive bacteria to better frame the time required to achieve the best result. Approximately 106 CFU/mL of Escherichia coli ATCC 25922; Salmonella Typhimurium ATCC 1402; Yersinia enterocolitica ATCC 9610; Pseudomonas aeruginosa ATCC 27588; Staphylococcus aureus ATCC 6538; Enterococcus faecalis ATCC 29212; Bacillus cereus ATCC 14579 and Listeria monocytogenes NCTT 10888 were inoculated onto each aluminum surface and challenged with UV and alcohol 70% at 0, 15", 30", 1', 5', 15', 30', 1, 2, 4 and 6 h. DURALTI® coating already confirmed its ability to induce a 4-logarithmic decrease (from 106 to 102 CFU/mL) after 6 h. Once each sanitizing treatment was applied, an overall bacterial inhibition occurred in a time ranging from 15'' to 1'. The results are innovative in terms of preventing microbial adhesion and growth in the food industry

Genomic-assisted characterisation of <i>Pseudomonas</i> sp. strain Pf4, a potential biocontrol agent in hydroponics

<p>In an attempt to select potential biocontrol agents against <i>Pythium</i> spp. and <i>Rhizoctonia</i> spp. root pathogens for use in soilless systems, 12 promising bacteria were selected for further investigations. Sequence analysis of the 16S rRNA gene revealed that three strains belonged to the genus <i>Enterobacter</i>, whereas nine strains belonged to the genus <i>Pseudomonas</i>. In <i>in vitro</i> assays, one strain of <i>Pseudomonas</i> sp., Pf4, closely related to <i>Pseudomonas protegens</i> (formerly <i>Pseudomonas fluorescens</i>), showed noteworthy antagonistic activity against two strains of <i>Pythium aphanidermatum</i> and two strains of <i>Rhizoctonia solani</i> AG 1-IB, with average inhibition of mycelial growth >80%. Strain Pf4 was used for <i>in vivo</i> treatments on lamb’s lettuce against <i>R. solani</i> root rot in small-scale hydroponics. Pf4-treated and untreated plants were daily monitored for symptom development and after two weeks of infection, a significant protective effect of Pf4 against root rot was recorded. The survival and population density of Pf4 on roots were also checked, demonstrating a density above the threshold value of 10⁵ CFU g⁻¹ of root required for disease suppression. Known loci for the synthesis of antifungal metabolites, detected using PCR, and draft-genome sequencing of Pf4 demonstrated that <i>Pseudomonas</i> sp. Pf4 has the potential to produce an arsenal of secondary metabolites (<i>plt</i>, <i>phl</i>, <i>ofa</i> and <i>fit-rzx</i> gene clusters) very similar to that of the well-known biocontrol <i>P. protegens</i> strain Pf-5.</p