Bats' Conquest of a Formidable Foraging Niche: The Myriads of Nocturnally Migrating Songbirds

Along food chains, i.e., at different trophic levels, the most abundant taxa often represent exceptional food reservoirs, and are hence the main target of consumers and predators. The capacity of an individual consumer to opportunistically switch towards an abundant food source, for instance, a prey that suddenly becomes available in its environment, may offer such strong selective advantages that ecological innovations may appear and spread rapidly. New predator-prey relationships are likely to evolve even faster when a diet switch involves the exploitation of an unsaturated resource for which few or no other species compete. Using stable isotopes of carbon and nitrogen as dietary tracers, we provide here strong support to the controversial hypothesis that the giant noctule bat Nyctalus lasiopterus feeds on the wing upon the multitude of flying passerines during their nocturnal migratory journeys, a resource which, while showing a predictable distribution in space and time, is only seasonally available. So far, no predator had been reported to exploit this extraordinarily diverse and abundant food reservoir represented by nocturnally migrating passerines

Intrapopulation Variability Shaping Isotope Discrimination and Turnover: Experimental Evidence in Arctic Foxes

Tissue-specific stable isotope signatures can provide insights into the trophic ecology of consumers and their roles in food webs. Two parameters are central for making valid inferences based on stable isotopes, isotopic discrimination (difference in isotopic ratio between consumer and its diet) and turnover time (renewal process of molecules in a given tissue usually measured when half of the tissue composition has changed). We investigated simultaneously the effects of age, sex, and diet types on the variation of discrimination and half-life in nitrogen and carbon stable isotopes (δ15N and δ13C, respectively) in five tissues (blood cells, plasma, muscle, liver, nail, and hair) of a top predator, the arctic fox Vulpes lagopus. We fed 40 farmed foxes (equal numbers of adults and yearlings of both sexes) with diet capturing the range of resources used by their wild counterparts. We found that, for a single species, six tissues, and three diet types, the range of discrimination values can be almost as large as what is known at the scale of the whole mammalian or avian class. Discrimination varied depending on sex, age, tissue, and diet types, ranging from 0.3‰ to 5.3‰ (mean = 2.6‰) for δ15N and from 0.2‰ to 2.9‰ (mean = 0.9‰) for δ13C. We also found an impact of population structure on δ15N half-life in blood cells. Varying across individuals, δ15N half-life in plasma (6 to 10 days) was also shorter than for δ13C (14 to 22 days), though δ15N and δ13C half-lives are usually considered as equal. Overall, our multi-factorial experiment revealed that at least six levels of isotopic variations could co-occur in the same population. Our experimental analysis provides a framework for quantifying multiple sources of variation in isotopic discrimination and half-life that needs to be taken into account when designing and analysing ecological field studies

Tracking Cats: Problems with Placing Feline Carnivores on δ18O, δD Isoscapes

Several felids are endangered and threatened by the illegal wildlife trade. Establishing geographic origin of tissues of endangered species is thus crucial for wildlife crime investigations and effective conservation strategies. As shown in other species, stable isotope analysis of hydrogen and oxygen in hair (δD(h), δ(18)O(h)) can be used as a tool for provenance determination. However, reliably predicting the spatial distribution of δD(h) and δ(18)O(h) requires confirmation from animal tissues of known origin and a detailed understanding of the isotopic routing of dietary nutrients into felid hair.We used coupled δD(h) and δ(18)O(h) measurements from the North American bobcat (Lynx rufus) and puma (Puma concolor) with precipitation-based assignment isoscapes to test the feasibility of isotopic geo-location of felidae. Hairs of felid and rabbit museum specimens from 75 sites across the United States and Canada were analyzed. Bobcat and puma lacked a significant correlation between H/O isotopes in hair and local waters, and also exhibited an isotopic decoupling of δ(18)O(h) and δD(h). Conversely, strong δD and δ(18)O coupling was found for key prey, eastern cottontail rabbit (Sylvilagus floridanus; hair) and white-tailed deer (Odocoileus virginianus; collagen, bone phosphate).Puma and bobcat hairs do not adhere to expected pattern of H and O isotopic variation predicted by precipitation isoscapes for North America. Thus, using bulk hair, felids cannot be placed on δ(18)O and δD isoscapes for use in forensic investigations. The effective application of isotopes to trace the provenance of feline carnivores is likely compromised by major controls of their diet, physiology and metabolism on hair δ(18)O and δD related to body water budgets. Controlled feeding experiments, combined with single amino acid isotope analysis of diets and hair, are needed to reveal mechanisms and physiological traits explaining why felid hair does not follow isotopic patterns demonstrated in many other taxa

Radiocarbon analysis of the early archaeological site of Nauwalabila I, Arnhem Land, Australia: implications for sample suitability and stratigraphic integrity

This study presents the results of an extensive radiocarbon dating program at the Nauwalabila I site in northern Australia. The results show that the radiocarbon chronology at Nauwalabila is reliable to similar to 130 cm depth, but below this depth coarse charcoal has been variably altered during a period in the early Holocene when an ephemeral groundwater table reached close to the ground surface of the time. Below similar to 150 cm none of the radiocarbon ages can be considered to indicate reliably the age of deposition of the sediments. Luminescence dates near the surface and at I 10 cm are concordant with the radiocarbon chronology in the upper part of the sequence, and hence the aberrant radiocarbon results below similar to 150 cm do not constitute a reason to doubt the accuracy of the luminescence chronology deeper in the stratigraphy.A conservative estimate of the age of the sequence, based on extrapolation of results from that portion of the sequence where the radiocarbon chronology is considered to be reliable, is consistent with the chronology proposed previously from luminescence dating. Both chronologies therefore suggest occupation of the site before 50,000 years. Based on sediment characteristics and the distribution of quartz, chert, quartzite and 'quartz crystal' artefacts, there is no evidence that there has been significant vertical displacement of artefacts relative to the surrounding sand matrix.Both chemical alteration and physical translocation of charcoal contributed to the aberrant ages at depth in the deposit. The results point to the need for careful assessment of the suitability of charcoal for radiocarbon dating prior to analysis and to the dangers of relying on a small number of radiocarbon dates in the development robust site chronologies. Strategies for screening samples for suitability include (i) microscopic examination, (ii) not analysing samples unless they survive the full ABOX pretreatment, (iii) not analysing samples unless the material is significantly larger than the sediment matrix, (iv) using CHN analysis on both untreated and pretreated material to check for organic contamination and (v) using stepped combustion to check for concordancy in the ages of carbon released at successively higher temperatures. (C) 2002 Elsevier Science Ltd. All rights reserved.</p

Radiocarbon analysis of the early archaeological site of Nauwalabila I, Arnhem Land, Australia: implications for sample suitability and stratigraphic integrity

This study presents the results of an extensive radiocarbon dating program at the Nauwalabila I site in northern Australia. The results show that the radiocarbon chronology at Nauwalabila is reliable to similar to 130 cm depth, but below this depth coarse charcoal has been variably altered during a period in the early Holocene when an ephemeral groundwater table reached close to the ground surface of the time. Below similar to 150 cm none of the radiocarbon ages can be considered to indicate reliably the age of deposition of the sediments. Luminescence dates near the surface and at I 10 cm are concordant with the radiocarbon chronology in the upper part of the sequence, and hence the aberrant radiocarbon results below similar to 150 cm do not constitute a reason to doubt the accuracy of the luminescence chronology deeper in the stratigraphy.A conservative estimate of the age of the sequence, based on extrapolation of results from that portion of the sequence where the radiocarbon chronology is considered to be reliable, is consistent with the chronology proposed previously from luminescence dating. Both chronologies therefore suggest occupation of the site before 50,000 years. Based on sediment characteristics and the distribution of quartz, chert, quartzite and 'quartz crystal' artefacts, there is no evidence that there has been significant vertical displacement of artefacts relative to the surrounding sand matrix.Both chemical alteration and physical translocation of charcoal contributed to the aberrant ages at depth in the deposit. The results point to the need for careful assessment of the suitability of charcoal for radiocarbon dating prior to analysis and to the dangers of relying on a small number of radiocarbon dates in the development robust site chronologies. Strategies for screening samples for suitability include (i) microscopic examination, (ii) not analysing samples unless they survive the full ABOX pretreatment, (iii) not analysing samples unless the material is significantly larger than the sediment matrix, (iv) using CHN analysis on both untreated and pretreated material to check for organic contamination and (v) using stepped combustion to check for concordancy in the ages of carbon released at successively higher temperatures. (C) 2002 Elsevier Science Ltd. All rights reserved.</p