High-responsivity vertical-illumination Si/Ge uni-traveling-carrier photodiodes based on silicon-on-insulator substrate

Si/Ge uni-traveling carrier photodiodes exhibit higher output current when space-charge effects are overcome and thermal effects are suppressed, which is highly beneficial for increasing the dynamic range of various microwave photonic systems and simplifying high-bit-rate digital receivers in different applications. From the point of view of packaging, detectors with vertical-illumination configuration can be easily handled by pick-and-place tools and are a popular choice for making photo-receiver modules. However, vertical-illumination Si/Ge uni-traveling carrier (UTC) devices suffer from inter-constraint between high speed and high responsivity. Here, we report a high responsivity vertical-illumination Si/Ge UTC photodiode based on a silicon-on-insulator substrate. The maximum absorption efficiency of the devices was 2.4 times greater than the silicon substrate owing to constructive interference. The Si/Ge UTC photodiode was successfully fabricated and had a dominant responsivity at 1550 nm of 0.18 A/W, a 50% improvement even with a 25% thinner Ge absorption layer.Comment: 5pages,2figure

Multi-task deep neural network acoustic models with model adaptation using discriminative speaker identity for whisper recognition

This paper presents a study on large vocabulary continuous whisper automatic recognition (wLVCSR). wLVCSR provides the ability to use ASR equipment in public places without concern for disturbing others or leaking private information. However the task of wLVCSR is much more challenging than normal LVCSR due to the absence of pitch which not only causes the signal to noise ratio (SNR) of whispers to be much lower than normal speech but also leads to flatness and formant shifts in whisper spectra. Furthermore, the amount of whisper data available for training is much less than for normal speech. In this paper, multi-task deep neural network (DNN) acoustic models are deployed to solve these problems. Moreover, model adaptation is performed on the multi-task DNN to normalize speaker and environmental variability in whispers based on discriminative speaker identity information. On a Mandarin whisper dictation task, with 55 hours of whisper data, the proposed SI multi-task DNN model can achieve 56.7% character error rate (CER) improvement over a baseline Gaussian Mixture Model (GMM), discriminatively trained only using the whisper data. Besides, the CER of the proposed model for normal speech can reach 15.2%, which is close to the performance of a state-of-the-art DNN trained with one thousand hours of speech data. From this baseline, the model-adapted DNN gains a further 10.9% CER reduction over the generic model

Involvement of Lysosome Membrane Permeabilization and Reactive Oxygen Species Production in the Necrosis Induced by Chlamydia muridarum Infection in L929 Cells

Chlamydiae, obligate intracellular bacteria, are associated with a variety of human diseases. The chlamydial life cycle undergoes a biphasic development: replicative reticulate bodies (RBs) phase and infectious elementary bodies (EBs) phase. At the end of the chlamydial intracellular life cycle, EBs have to be released to the surrounded cells. Therefore, the interactions between Chlamydiae and cell death pathways could greatly influence the outcomes of Chlamydia infection. However, the underlying molecular mechanisms remain elusive. Here, we investigated host cell death after Chlamydia infection in vitro, in L929 cells, and showed that Chlamydia infection induces cell necrosis, as detected by the propidium iodide (PI)-Annexin V double-staining flow-cytometric assay and Lactate dehydrogenase (LDH) release assay. The production of reactive oxygen species (ROS), an important factor in induction of necrosis, was increased after Chlamydia infection, and inhibition of ROS with specific pharmacological inhibitors, diphenylene iodonium (DPI) or butylated hydroxyanisole (BHA), led to significant suppression of necrosis. Interestingly, live-cell imaging revealed that Chlamydia infection induced lysosome membrane permeabilization (LMP). When an inhibitor upstream of LMP, CA-074-Me, was added to cells, the production of ROS was reduced with concomitant inhibition of necrosis. Taken together, our results indicate that Chlamydia infection elicits the production of ROS, which is dependent on LMP at least partially, followed by induction of host-cell necrosis. To our best knowledge, this is the first live-cell-imaging observation of LMP post Chlamydia infection and report on the link of LMP to ROS to necrosis during Chlamydia infection. </p

The Research and Application of New Horizontal Well for Offshore Oilfield Thin Reservoir Based on Pseudo Steady State Time

Fluids rate forecast of horizontal well are particularly important as horizontal well technology is widely used in bohai oilfield. According to the sector planning, horizontal well productivity is two to five times as much as directional well, combined with drawdown pressure, reservoir thickness and permeability to determine the horizontal well productivity. But this method lack of theoretical basis and need reservoir personnel has rich experience at the same time the compute number is the fixed value that can’t reflect time dependent process of horizontal well productivity. In this paper a new mathematical model is constructed and introduced investigation radius. Based on these a new equation is derived. This theory can provide valuable for horizontal well productivity during a period of time, at the same time it can judgment whether the reservoir existing skin through production performance

Sulfadiazine Sodium Ameliorates the Metabolomic Perturbation in Mice Infected with Toxoplasma gondii

In this study, we analyzed the global metabolomic changes associated with Toxoplasma gondii infection in mice in the presence or absence of sulfadiazine sodium (SDZ) treatment. BALB/c mice were infected with T. gondii GT1 strain and treated orally with SDZ (250 g/ml in water) for 12 consecutive days. Mice showed typical manifestations of illness at 20 days postinfection (dpi); by 30 dpi, 20% had survived and developed latent infection. We used ultraperformance liquid chromatography-mass spectrometry to profile the serum metabolomes in control (untreated and uninfected) mice, acutely infected mice, and SDZ-treated and infected mice. Infection induced significant perturbations in the metabolism of-linolenic acid, purine, pyrimidine, arginine, tryptophan, valine, glycerophospholipids, and fatty acyls. However, treatment with SDZ seemed to alleviate the serum metabolic alterations caused by infection. The restoration of the serum metabolite levels in the treated mice was associated with better clinical outcomes. These data indicate that untargeted metabolomics can reveal biochemical pathways associated with restoration of the metabolic status of T. gondii-infected mice following SDZ treatment and could be used to monitor responses to SDZ treatment. This study provides a new systems approach to elucidate the metabolic and therapeutic effects of SDZ in the context of murine toxoplasmosis. K E Y W O R D S Toxoplasma gondii, biomarkers, metabolomics, mice, serum metabolites, sulfadiazine sodium Toxoplasma gondii, an obligate intracellular protozoan parasite, is highly prevalent in warm-blooded animals and humans (1). T. gondii comprises three clonal lineages (type I, type II, and type III) (2). Despite 98% genetic similarity, dramatic differences in virulence exist among strains belonging to these T. gondii genotypes (3). Humans acquire infection mainly by ingesting undercooked meat containing tissue cysts or oocysts from contaminated water (4). Acute infection with this parasite is mediated by the aggressive, fast-replicating, tachyzoite stage, which can cause encephalitis or retinochoroiditis. In addition, reactivation of the latent form (i.e., bradyzoites-containing cysts) of T. gondii can cause life-threatening conditions and even death in immuno-compromised individuals (5)