32 research outputs found

    Perturbation of magnetostatic modes observed by ferromagnetic resonance force microscopy

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    Magnetostatic modes of yttrium iron garnet (YIG) films are investigated by ferromagnetic resonance force microscopy. A thin-film "probe" magnet at the tip of a compliant cantilever introduces a local inhomogeneity in the internal field of the YIG sample. This influences the shape of the sample's magnetostatic modes, thereby measurably perturbing the strength of the force coupled to the cantilever. We present a theoretical model that explains these observations; it shows that the tip-induced variation of the internal field creates either a local "potential barrier" or "potential well" for the magnetostatic waves. The data and model together indicate that local magnetic imaging of ferromagnets is possible, even in the presence of long-range spin coupling, through the introduction of localized magnetostatic modes predicted to arise from sufficiently strong tip fields

    Understorey plant community and light availability in conifer plantations and natural hardwood forests in Taiwan

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    Questions: What are the effects of replacing mixed species natural forests with Cryptomeria japonica plantations on understorey plant functional and species diversity? What is the role of the understorey light environment in determining understorey diversity and community in the two types of forest? Location: Subtropical northeast Taiwan. Methods: We examined light environments using hemispherical photography, and diversity and composition of understorey plants of a 35‐yr C. japonica plantation and an adjacent natural hardwood forest. Results: Understorey plant species richness was similar in the two forests, but the communities were different; only 18 of the 91 recorded understorey plant species occurred in both forests. Relative abundance of plants among different functional groups differed between the two forests. Relative numbers of shade‐tolerant and shade‐intolerant seedling individuals were also different between the two forest types with only one shade‐intolerant seedling in the plantation compared to 23 seedlings belonging to two species in the natural forest. In the natural forest 11 species of tree seedling were found, while in the plantation only five were found, and the seedling density was only one third of that in the natural forest. Across plots in both forests, understorey plant richness and diversity were negatively correlated with direct sunlight but not indirect sunlight, possibly because direct light plays a more important role in understorey plant growth. Conclusions: We report lower species and functional diversity and higher light availability in a natural hardwood forest than an adjacent 30‐yr C. japonica plantation, possibly due to the increased dominance of shade‐intolerant species associated with higher light availability. To maintain plant diversity, management efforts must be made to prevent localized losses of shade‐adapted understorey plants

    Increasing the PLED Luminescence Efficiency by Exploiting the Surface Plasmon Resonance Effect

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    Thermal evaporation was used to fabricate 60-nm Au particles sandwiched between a self-made nitrogen-doped aluminum zinc oxide film and a glass substrate to form novel window materials for use in polymer light emitting diodes (PLEDs). The PLEDs considered herein are single-layer devices based on a poly [2-methoxy,5-(2'-ethyl-hexyloxy)-1,4-phenylene vinylene] emissive layer. In addition to its low cost, this novel fabrication method can effectively prevent interruption or degradation of the charge transport properties of the active layer to meet the high performance requirements of PLEDs. The luminance-voltage measurements show that the absorption band of the Au nanoparticles due to the surface plasmon resonance effect increased the general luminescence efficiency of the PLED device by 60%. (C) 2010 The Electrochemical Society. [DOI: 10.1149/1.3527981] All rights reserved

    Unstable systems database: A new tool for students, teachers and scientists

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    The contribution presents a starting project of a site focused on unstable systems. It is a web-based database in the bilingual version (ENG/CZ) which can be used as an information database for models of unstable processes. The site contains mathematical models of such systems including their simulation files together with basic information about stability of dynamical systems. The paper outlines motivation for development of this database, presents its basic structure and discusses several models from the site. Areas of prospective usage are also suggested together with possible directions of further development of this project. © Springer International Publishing Switzerland 2013

    Spinal glutamatergic NMDA-dependent pelvic nerve-to-external urethra sphincter reflex potentiation caused by a mechanical stimulation in anesthetized rats

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    The current study investigates whether the spinal pelvic nerve-to-external urethra sphincter (EUS) reflex potentiation can be induced by a mechanical stimulation and whether the glutamatergic mechanism is involved in yielding such a reflex potentiation. The external urethra sphincter electromyogram (EUSE) activity, evoked by a single or by repetitive pelvic nerve stimulation, in 30 anesthetized rats was recorded with/without bladder saline distension. Without saline distension (0 cmH(2)O), a single pulse nerve stimulation evoked a single action potential in the reflex activity, whereas repetitive pelvic stimulation and saline distension (6 similar to 20 cmH(2)O) both elicited a long-lasting reflex potentiation (20.05 +/- 3.21 and 75.01 +/- 9.87 spikes/stimulation, respectively). The saline distension-induced pelvic nerve-to-EUS reflex potentiation was abolished by D-2-amino-5-phosphonovalerate [APV; a glutamatergic N-methyl-D-aspartic acid ( NMDA) receptor antagonist; 100 mu M, 10 mu l, 1.72 +/- 0.31 spikes/stimulation] and attenuated by 2,3-dihydroxy-6-nitro-7-sulfamoylbenzo ( F) quinoxaline [ NBQX; a glutamatergic alpha-amino-3-hydroxy-5-methyl-4- isoxazoleproprionate ( AMPA) receptor antagonist; 100 mu M, 10 mu l, 26.16 +/- 7.27 spikes/stimulation], but was not affected by bicuculline (a GABAergic antagonist; 100 mu M, 10 mu l, 53.62 +/- 15.54 spikes/stimulation). Intrathecal administration of glutamate (31.12 +/- 8.25 spikes/stimulation, 100 mu M, 10 mu l) and NMDA (26.25 +/- 4.12 spikes/stimulation, 100 mu M, 10 mu l) both induced a long-lasting pelvic nerve-to-EUS reflex potentiation without saline distension, which was similar to the findings observed from saline distension only. The duration of the contraction wave of the urethra was elongated by the saline distension-induced pelvic nerve-to-EUS reflex potentiation, whereas the peak pressure of the contraction wave was not affected. Our findings suggest that saline distension in the bladder elicits a pelvic nerve-to-EUS reflex potentiation and the glutamatergic mechanism contributes to the presence of such a reflex potentiation

    Transcription repressor Slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma

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    Purpose: In a previous genome-wide gene expression profiling analysis using an invasion cancer cell lines model, we have identified Slug as selectively overexpressed in the highly invasive cancer cells. Here, we investigated the clinical significance of Slug in lung adenocarcinoma and the role of Slug in the process of cancer cell invasion and metastasis. Experimental Design: Real-time quantitative reverse transcription-PCR was used to investigate Slug mRNA in surgically resected lung adenocarcinoma of 54 patients and its correlation with survival. We overexpressed Slug in a lung adenocarcinoma cell line with very low Slug levels and investigated the in vitro and in vivo effects of Slug expression. Results: High expression of Slug mRNA in lung cancer tissue was significantly associated with postoperative relapse (P = 0.03) and shorter patient survival (P < 0.001). The overexpression of Slug enhanced xenograft tumor growth and increased microvessel counts in angiogenesis assay. Both inducible and constitutive overexpression of Slug suppressed the expression of E-cadherin and increased the in vitro invasive ability. Zymography revealed increased matrix metalloproteinase-2 activity in Slug overexpressed cells. ELISA, reverse transcription-PCR, and immunohistochemistry confirmed the increase of matrix metalloproteinase-2 proteins and mRNA in Slug overexpressed cells and xenograft tumors. Conclusions: Slug expression can predict the clinical outcome of lung adenocarcinoma patients. Slug is a novel invasion-promoting gene in lung adenocarcinoma

    Expressed transcripts associated with high rates of egg production in chicken ovarian follicles

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    The purpose of this study was to characterize differentially expressed transcripts associated with varying rates of egg production in Taiwan country chickens. Ovarian follicles were isolated from two strains of chicken which showed low (B) or high (L2) rates of egg production, then processed for RNA extraction and cDNA library construction. Three thousand and eight forty clones were randomly selected from the cDNA library and amplified by PCR, then used in microarray analysis. Differentially expressed transcripts (P = 1.75) were sequenced, and aligned using GenBank. This analysis revealed 20 non-redundant sequences which corresponded to known transcripts. Eight transcripts were expressed at a higher level in ovarian tissue prepared from chicken strain B, and 12 transcripts were expressed at a higher level in L2 birds. These differential patterns of expression were confirmed by semi-quantitative RT-PCR. We show that transcripts of cyclin B2 (cycB2), ferritin heavy polypeptide 1 (FTH1), Gag-Pol polyprotein, thymosin beta 4 (TB4) and elongation factor 1 alpha 1 (EEF1A1) were enriched in B strain ovarian follicles. In contrast, thioredoxin (TXN), acetyl-CoA dehydrogenase long chain (ACADL), inhibitor of growth family member 4 (ING4) and annexin II (ANXA2) were expressed in at higher levels in the L2 strain. We suggest that our approach may lead to the isolation of effective molecular markers that can be used in selection programs in Taiwan country chickens. (C) 2007 Elsevier Ltd. All rights reserved
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