Bistability of cell-matrix adhesions resulting from non-linear receptor-ligand dynamics

Bistability is a major mechanism for cellular decision making and usually results from positive feedback in biochemical control systems. Here we show theoretically that bistability between unbound and bound states of adhesion clusters results from positive feedback mediated by structural rather than biochemical processes, namely by receptor-ligand dissociation and association dynamics which depend non-linearly on mechanical force and receptor-ligand separation. For small cell-matrix adhesions, we find rapid switching between unbound and bound states, which in the initial stages of adhesion allows the cell to explore its environment through many transient adhesions.Comment: Revtex, 3 pages, 3 postscript figures included, to appear in Biophysical Journal as Biophysical Lette

Tubular structures of GaS

In this Brief Report we demonstrate, using density-functional tight-binding theory, that gallium sulfide (GaS) tubular nanostructures are stable and energetically viable. The GaS-based nanotubes have a semiconducting direct gap which grows towards the value of two-dimensional hexagonal GaS sheet and is in contrast to carbon nanotubes largely independent of chirality. We further report on the mechanical properties of the GaS-based nanotubes

Consent and Dissent: A Study of the Reaction of Chinese School Teachers in Guangzhou City Schools to Government Educational Reforms

This paper presents detailed qualitative evidence from a case study of teachers in five Chinese schools in one city. It explicitly seeks to show how developments in government policy towards education have altered the management of teacher labour inside schools as well as the teacher labour process as expressed by the teachers themselves in interviews and questionnaires. In this paper, we explore supervision, work intensification, and the erosion of professionalism. We conclude that some changes have taken place as predicted by the labour process model, but that the reaction of the teachers to more extensive controls has been variable. In particular senior school managers did have greater control with high levels of supervision, but that was generally welcomed as preferable to the previous system of outside control and neglect. While workload increased overall, the teachers were more likely to have to work outside of normal duties rather than experience any increase in formal contractual obligations

RF Sensing for Continuous Monitoring of Human Activities for Home Consumer Applications

Radar for indoor monitoring is an emerging area of research and development, covering and supporting different health and wellbeing applications of smart homes, assisted living, and medical diagnosis. We report on a successful RF sensing system for home monitoring applications. The system recognizes Activities of Daily Living(ADL) and detects unique motion characteristics, using data processing and training algorithms. We also examine the challenges of continuously monitoring various human activities which can be categorized into translation motions (active mode) and in-place motions (resting mode). We use the range-map, offered by a range-Doppler radar, to obtain the transition time between these two categories, characterized by changing and constant range values, respectively. This is achieved using the Radon transform that identifies straight lines of different slopes in the range-map image. Over the in-place motion time intervals, where activities have insignificant or negligible range swath, power threshold of the radar return micro-Doppler signatures,which is employed to define the time-spans of individual activities with insignificant or negligible range swath. Finding both the transition times and the time-spans of the different motions leads to improved classifications, as it avoids decisions rendered over time windows covering mixed activities.Comment: 12 page

Natural and Induced Mitochondrial Phosphate Carrier Loss: DIFFERENTIAL DEPENDENCE OF MITOCHONDRIAL METABOLISM AND DYNAMICS AND CELL SURVIVAL ON THE EXTENT OF DEPLETION.

The relevance of mitochondrial phosphate carrier (PiC), encoded by SLC25A3, in bioenergetics is well accepted. However, little is known about the mechanisms mediating the cellular impairments induced by pathological SLC25A3 variants. To this end, we investigated the pathogenicity of a novel compound heterozygous mutation in SLC25A3 First, each variant was modeled in yeast, revealing that substituting GSSAS for QIP within the fifth matrix loop is incompatible with survival on non-fermentable substrate, whereas the L200W variant is functionally neutral. Next, using skin fibroblasts from an individual expressing these variants and HeLa cells with varying degrees of PiC depletion, PiC loss of ∼60% was still compatible with uncompromised maximal oxidative phosphorylation (oxphos), whereas lower maximal oxphos was evident at ∼85% PiC depletion. Furthermore, intact mutant fibroblasts displayed suppressed mitochondrial bioenergetics consistent with a lower substrate availability rather than phosphate limitation. This was accompanied by slowed proliferation in glucose-replete medium; however, proliferation ceased when only mitochondrial substrate was provided. Both mutant fibroblasts and HeLa cells with 60% PiC loss showed a less interconnected mitochondrial network and a mitochondrial fusion defect that is not explained by altered abundance of OPA1 or MFN1/2 or relative amount of different OPA1 forms. Altogether these results indicate that PiC depletion may need to be profound (\u3e85%) to substantially affect maximal oxphos and that pathogenesis associated with PiC depletion or loss of function may be independent of phosphate limitation when ATP requirements are not high

The analysis on the single particle model of CDW

Gruner put forward a single particle model of charge-density wave, which is a typical nonlinear differential equation, and also a mathematical model of pendulum. This Letter analyzes the solution of equation by the rotated vector fields theory, providing the relation between the applied field E and the periodic solution, and the conclusion that the critical value of E for the periodic solution is fixed in the over-damped situation. With these conclusions, it derives the formulae of nonlinear conductivity, narrow-band noise, which are consistent with the empirical ones given by Fleming.Comment: This is a version with a physics focus, the part with a mathematical focus is submitted at arXiv:0807.328