Alcaloides Indoliques. VII. Structure de la Voacoline

Voacoline, a new minor alkaloid from Voacanga chalotiana Pierre ex Stapf (apocynaceae) is shown to have structure I. This structure contains two unusual features :a Na‐CH3 indolic group and a hemiketal function. This last feature accounts for the very easy formation of O‐methyl II and O‐ethyl‐voacoline HI. A correlation with voachalotine IV, the major alkaloid of the same species, has been achieved by degradation of I to dihydrodeshydroxymethylvoachalotine XIV. UV, IR, NMR and mass spectra of I and some of its derivatives are discussed. Copyright © 1965 Wiley‐VCH Verlag GmbH & Co. KGaA, WeinheimSCOPUS: ar.jinfo:eu-repo/semantics/publishe

Periostin as a Biomarker of the Amniotic Membrane

Tracing the precise developmental origin of amnion and amnion-derived stem cells is still challenging and depends chiefly on analyzing powerful genetic model amniotes like mouse. Profound understanding of the fundamental differences in amnion development in both the disc-shaped primate and human embryo and the cup-shaped mouse embryo is pivotal in particular when sampling amniotic membrane from nonprimate species for isolating candidate amniotic stem cells. The availability of molecular marker genes that are specifically expressed in the amniotic membrane and not in other extraembryonic membranes would be instrumental to validate unequivocally the starting material under investigation. So far such amniotic markers have not been reported. We postulated that bone morphogenetic protein (BMP) target genes are putative amniotic membrane markers mainly because deficiency in one of several components of the BMP signaling cascade in mice has been documented to result in defective development of the early amnion. Comparative gene expression analysis of acknowledged target genes for BMP in different extraembryonic tissues, combined with in situ hybridization, identified Periostin (Postn) mRNA enrichment in amnion throughout gestation. In addition, we identify and propose a combination of markers as transcriptional signature for the different extraembryonic tissues in mouse