FACTORS RELATED TO ORAL CANDIDIASIS IN ELDERLY USERS AND NON-USERS OF REMOVABLE DENTAL PROSTHESES

This study examined the association between oral candidiasis in elderly users and nonusers of prosthesis and its predisposing factors. To this end, we performed a cross-sectional study where saliva samples from 48 patients were collected they used prosthesis and 43 patients (control group) who did not use. Among the 91 patients, Candida spp were isolated in 40 (83.3%) who used prosthesis and in 23 (53.5%) in the control group. A statistically significant association was determined between the two groups, the isolation of yeasts and dental prosthesis (p < 0.05, OR = 4.3). The most common etiological agent was Candida albicans (37 isolates), with 23 (62.2%) in the denture group and 14 (37.8%) (control group). Among patients who presented clinical manifestations of oral candidiasis (n = 24), 83.3% (n = 20) belonged to the group that wore dentures, while only 16.7% (n = 4) belonged to the control group. Elderly patients with diabetes had 4.4 times higher estimated risk of developing oral candidiasis when compared with individuals without this condition. There was no statistically significant association between being user prostheses and have diabetes with the onset of candidiasis. No statistically significant association was determined between xerostomia, use of prosthesis and oral candidiasis. The use of prosthetics and poor oral hygiene in elderly patients predisposes to the development of oral candidiasis

Species composition and fauna distribution of mosquitoes (Diptera: Culicidae) and its importance for vector-borne diseases in a rural area of Central Western - Mato Grosso, Brazil

Abstract. This study describes ecological data obtained in a rural area in the State of Mato Grosso, including the insects belonging to the family Culicidae, especially those framed as potential vectors of tropical diseases. In 2015, we collected adult mosquitoes in fragments of forest in a rural area located in Mato Grosso Central West of Brazil. We captured 18,256 mosquitoes of the sub-families Culicinae and Anophelinae and have identified 34 species belonging to 12 genera: Aedes (1 species), Anopheles (8 species), Coquillettidia (1 species), Haemagogus (1 species), Culex (5 species), Psorophora  (5 species), Ochlerotatus (4 species), Deinocerites (1 species),  Mansonia (4 species), Sabethes (2 species), Limatus (1 species), Wyeomyia (1 species). The family Culicidae presented high richness and abundance, established by diversity indexes (Margalef α =3.26; Shannon H' = 2.09; Simpson D = 0.19) with dominance of the species Anopheles (Nyssorhyncus) darlingi Root (89.8%). This species has considerable epidemiological value, considered the main vector of malaria in Mato Grosso. Many species of mosquitoes are vectors of pathogens that cause disease in humans and domestic animals, transmitting pathogens including viruses (arboviruses), filaria worms (helminths) and protozoa. Composição de espécies e distribuição da fauna de mosquitos (Diptera: Culicidae) e sua importância para doenças transmitidas por vetores em uma área rural do centro-ocidental - Mato Grosso, Brasil Resumo. Este estudo descreve dados ecológicos de uma área rural do Estado de Mato Grosso e dos insetos da família Culicidae especialmente aqueles enquadrados como vetores potenciais de doenças tropicais. Em 2015, coletamos mosquitos adultos em fragmentos de floresta em localidades de áreas rurais no Mato Grosso região Centro Oeste do Brasil. Foram capturados 18.256 exemplares alados de mosquitos das subfamílias Culicinae e Anophelinae e identificadas 34 espécies pertencentes a 12 gêneros: Aedes (1 espécie), Anopheles (8 espécies), Coquillettidia (1 espécie), Haemagogus (1 espécie), Culex (5 espécies), Psorophora (5 espécies), Ochlerotatus (4 espécies), Deinocerites (1 espécie), Mansonia (4 espécies), Sabethes (2 espécies), Limatus (1 espécie), Wyeomyia (1 espécie). A família Culicidae apresentou alta riqueza e abundância, estabelecida por índices de diversidade (Margalef α = 3.26, Shannon H' = 2.09, Simpson D = 0.19) com predominância da espécie Anopheles (Nyssorhyncus)darlingi Root (89.8%). Esta espécie tem considerável valor epidemiológico, sendo considerada o principal vetor de malária em Mato Grosso. Muitas espécies de mosquitos são vetores de patógenos que causam doenças em humanos e animais domésticos, transmitindo patógenos incluindo vírus (arboviroses), filárias (helmintos) e protozoários

Entomological profile and new registers of the genera Anopheles (Diptera, Culicidae) in a Brazilian rural community of the District of Coxipó do Ouro, Cuiabá, Mato Grosso

The order Diptera is constituted of insects that possess numerous varieties of habitats, these winged, commonly called mosquitoes, comprise a monophyletic group. Malaria transmitters in Brazil are represented by mosquitoes of the Anopheles genus, being it principal vector species Anopheles (Nyssorhynchus) darlingi Root. Collectings were accomplished in the rural area of Cuiabá in the region of Coxipó do Ouro/MT, and a total 4,773 adult mosquitoes of the genus Anopheles were obtained. The prevailing species in the collectings where An. (Nys.) darlingi with 3,905 (81.8%), considered the vector of major epidemiological expression in the region, followed by Anopheles (Nyssorhynchus) argyritarsis (Robineau-Desvoidy) 267 (5.6%) and Anopheles (Nyssorhynchus) triannulatus (Neiva &amp; Pinto) 226 (4.7%). This report might be useful to entomological surveillance, demonstrating that the Coxipó do Ouro/MT locality might be elected as an area to be monitored, once the presence of such vector in this type of environment indicates a potential malaria transmission risk for the neighbouring regions in the state of Mato Grosso

Temporal distribution of Aedes aegypti Linnaeus (Diptera, Culicidae), in a Hospital in Cuiabá, State of Mato Grosso, Brazil

This study aimed at registering and monitoring the presence of Aedes aegypti in the University Hospital Júlio Muller, Cuiabá-MT, as well as investigating the influence of temperature and rainfall on its temporal distribution and egg densities in ovitraps. The study was performed from April/2007 to March/2008, utilizing ovitraps with 10% of hay infusion and a wood paddle as an oviposition substrate. For surveillance, one ovitrap was placed in each of the 12 points distributed throughout the hospital. Ovitraps were collected monthly at the end of a 5-day installation period. After egg counting, wood paddles were immersed in water to allow larval eclosion for species identification through optical microscopy. Egg Density Index (EDI), Positive Ovitraps Index (POI), and Mean Number of Eggs (MNE) were used for data analysis. The presence of A. aegypti in the hospital was registered throughout the study period, except in July. The MNE was proportionally higher in the internal area (n= 8.47 eggs/paddle) when compared to the external area (n= 5.46 eggs/paddle), and was higher in September/October 2007 and January/February 2008. A significant increase in EDI, POI and MNE was registered in periods where the average temperature was higher, and the increase in POI was also concomitant with an increase in rainfall. The continuous presence of A. aegypti in the hospital throughout the study period, points out the need of including this mosquito in the arthropod control list in this environment. This is particularly important, considering that A. aegypti is an important vector of several arboviroses

Species composition and fauna distribution of mosquitoes (Diptera: Culicidae) and its importance for vector-borne diseases in a rural area of Central Western - Mato Grosso, Brazil

Este estudo descreve dados ecológicos de uma área rural do Estado de Mato Grosso e dos insetos da família Culicidae especialmente aqueles enquadrados como vetores potenciais de doenças tropicais. Em 2015, coletamos mosquitos adultos em fragmentos de floresta em localidades de áreas rurais no Mato Grosso região Centro Oeste do Brasil. Foram capturados 18.256 exemplares alados de mosquitos das subfamílias Culicinae e Anophelinae e identificadas 34 espécies pertencentes a 12 gêneros: Aedes (1 espécie), Anopheles (8 espécies), Coquillettidia (1 espécie), Haemagogus (1 espécie), Culex (5 espécies), Psorophora (5 espécies), Ochlerotatus (4 espécies), Deinocerites (1 espécie), Mansonia (4 espécies), Sabethes (2 espécies), Limatus (1 espécie), Wyeomyia (1 espécie). A família Culicidae apresentou alta riqueza e abundância, estabelecida por índices de diversidade (Margalef α = 3.26, Shannon H' = 2.09, Simpson D = 0.19) com predominância da espécie Anopheles (Nyssorhyncus)darlingi Root (89.8%). Esta espécie tem considerável valor epidemiológico, sendo considerada o principal vetor de malária em Mato Grosso. Muitas espécies de mosquitos são vetores de patógenos que causam doenças em humanos e animais domésticos, transmitindo patógenos incluindo vírus (arboviroses), filárias (helmintos) e protozoários.This study describes ecological data obtained in a rural area in the State of Mato Grosso, including the insects belonging to the family Culicidae, especially those framed as potential vectors of tropical diseases. In 2015, we collected adult mosquitoes in fragments of forest in a rural area located in Mato Grosso Central West of Brazil. We captured 18,256 mosquitoes of the sub-families Culicinae and Anophelinae and have identified 34 species belonging to 12 genera: Aedes (1 species), Anopheles (8 species), Coquillettidia (1 species), Haemagogus (1 species), Culex (5 species), Psorophora (5 species), Ochlerotatus (4 species), Deinocerites (1 species), Mansonia (4 species), Sabethes (2 species), Limatus (1 species), Wyeomyia (1 species). The family Culicidae presented high richness and abundance, established by diversity indexes (Margalef α =3.26; Shannon H' = 2.09; Simpson D = 0.19) with dominance of the species Anopheles (Nyssorhyncus) darlingi Root (89.8%). This species has considerable epidemiological value, considered the main vector of malaria in Mato Grosso. Many species of mosquitoes are vectors of pathogens that cause disease in humans and domestic animals, transmitting pathogens including viruses (arboviruses), filaria worms (helminths) and protozoa

Utilização de implante ósseo cortical alógeno conservado em glicerina para preservação de membro torácico: estudo experimental em cães A limb sparing surgery with the use of a cortical allograft preserved in glicerin: an experimental study in dogs

A técnica de preservação de membro é uma alternativa à amputação em cães com osteossarcoma do esqueleto apendicular. OBJETIVO: O desenvolvimento da técnica preservadora aos moldes da original descrita por Straw¹. MÉTODOS: O procedimento cirúrgico foi realizado com modificações, substituindo-se o método de conservação dos implantes pela glicerina e o cimento ósseo utilizado no preenchimento do canal medular do implante pela poliuretana de mamona, e para avaliação da incorporação do implante foram feitos exames clínico, radiográfico e histopatológico em diferentes tempos pós-operatórios. RESULTADOS: Observada a boa função do membro no pós-operatório, a formação de calo ósseo e fechamento das linhas de interfaces com remodelação óssea em avaliação radiográfica e ao exame histopatológico foi notada a reabsorção do implante ósseo com sua substituição por tecido conjuntivo fibroso e tecido ósseo compacto, neovascularização e presença de infiltrado inflamatório plasmocitário. CONCLUSÕES: A poliuretana de mamona mostrou-se uma boa substituta ao cimento originalmente utilizado, aumentando a resistência do implante, sem causar reações do tipo corpo estranho e sem sua osteointegração. O implante ósseo conservado em glicerina apresentou ação osteoindutora e osteocondutora, sem sinais de rejeição; desta forma, a glicerina mostrou ser uma meio alternativo viável. Portanto, a técnica de preservação do membro, com suas modificações, caracterizou-se como alternativa à técnica original e à amputação do membro para cães com osteossarcoma de esqueleto apendicular.<br>A limb-sparing surgery can be an alternative treatment to amputation for dogs with appendicular osteosarcoma. PURPOSE: The purpose of this study was the development of this surgical technique, similar to the original, described by Straw¹, with changes in the procedure of the allografts preservation, and in the bone cement chose to be used intramedullary. METHODS: The surgery was performed in six health dogs, with the osteotomy of one-third of the radio and ulna, section of the distal margin in the radio-carpal joint and reconstruction using a cortical allograft preserved in 98% glycerin. The allograft's medullar channel was filled with a polymer extracted from castor oil (Ricinus communis) and a long dynamic compression plate were used to fix this allograft on the radio, carpal joint and 3rd metacarpal bone, with the arthrodesis of the carpal joint. A medio-lateral radiographs was performed immediately posteoperatively and with 7, 15, 30, 45, 60, 90, 120 and 150 days after surgery, to evaluate the allograft incorporation. The histopatological evaluation was done with 90, 120 and 150 days after surgery. RESULTS: Was notice a bone callus formation on the radiogrhphs exams by 60 days after surgery and bone bridges were closing the interfragmentary space, with remodeling by 90 days. We have noticed resorption of the grapht, with formation of a connective tissue and new bone around the polymer on the histophatological evaluation. A neovascularization and inflammatory cells in the connective tissue was noticed with a decreased in the inflammatory process with the time. CONCLUSIONS: The polymer extracted from castor oil showed to be a good substitute for the bone cement originally used in the limb-sparing surgery, increasing the graft resistance with no foreign body reaction or osteointegration. The allograft preserved in glycerin presented onteoinduction and osteocondution actions, with new bone formation, and no signs of rejection, then, the glycerin showed to be a good alternative preservation procedure. Therefore, the modified technique can be considered an alternative to the original limb-sparing surgery and to amputation for dogs with appendicular osteosarcoma, studies still deserve to be done with sick animals