24 research outputs found
First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid
Background: in a previous study, we detected the presence of a Mycobacterium avium species-specific insertion sequence, IS1245, in Mycobacterium kansasii. Both species were isolated from a mixed M. avium-M. kansasii bone marrow culture from an HIV-positive patient. the transfer mechanism of this insertion sequence to M. kansasii was investigated here.Methodology/Principal Findings: A linear plasmid (pMA100) was identified in all colonies isolated from the M. avium-M. kansasii mixed culture carrying the IS1245 element. the linearity of pMA100 was confirmed. Other analyses suggested that pMA100 contained a covalently bound protein in the terminal regions, a characteristic of invertron linear replicons. Partial sequencing of pMA100 showed that it bears one intact copy of IS1245 inserted in a region rich in transposase-related sequences. These types of sequences have been described in other linear mycobacterial plasmids. Mating experiments were performed to confirm that pMA100 could be transferred in vitro from M. avium to M. kansasii. pMA100 was transferred by in vitro conjugation not only to the M. kansasii strain from the mixed culture, but also to two other unrelated M. kansasii clinical isolates, as well as to Mycobacterium bovis BCG Moreau.Conclusions/Significance: Horizontal gene transfer (HGT) is one of most important mechanisms leading to the evolution and diversity of bacteria. This work provides evidence for the first time on the natural occurrence of HGT between different species of mycobacteria. Gene transfer, mediated by a novel conjugative plasmid, was detected and experimentally reproduced.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Cooperacion Interuniversitaria UAM-Banco Santander con America Latina (CEAL), UAM, SpainConselho Nacional de Desenvolvimento CientÃfico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de NÃvel Superior (CAPES)Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, BrazilLab Nacl Comp Cient, Petropolis, BrazilUniv Autonoma Madrid, Fac Med, Dept Prevent Med, Madrid, SpainInst Adolfo Lutz Registro, Nucleo TB & Micobacterioses, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, BrazilFAPESP: FAPESP - 06/01533-9Web of Scienc
Augmented Reality, Surgical Navigation, and 3D Printing for Transcanal Endoscopic Approach to the Petrous Apex
Optimization of 3D Print Material for the Recreation of Patient-Specific Temporal Bone Models
Oncologic and clinical outcomes in pelvic primary bone sarcomas treated with limb salvage surgery
Genotype and fetal size affect maternal–fetal amino acid status and fetal endocrinology in Large White×Landrace and Meishan pigs
Accuracy and Precision in Computer-Assisted Methods for Orthopaedic Surgery
This chapter reviews the major approaches to the definition of bone cutting accuracy in the field of computer-assisted orthopaedic surgery. The first part of the chapter reviews the different concepts of accuracy found in literature, from localization in image-guided systems to osteotomy accuracy evaluation both using navigation systems and in patient-specific instruments. The second part of the chapter focuses in the efforts toward the standardization of different computer-assisted accuracy measurements in orthopaedic surgery
Recommended from our members
South African National Survey of Research and Experimental Development: main analysis report 2011/12
Commissioned by the Department of Science and Technology, MarchThis report provides trends and a brief analysis of the survey data of the 2011/12 South African National Survey of Research and Experimental Development (R&D survey)