Aquaporin 1 (Aqp1) expression in healthy dog tears

Aquaporins (AQPs) are a family of thirteen membrane proteins that play an essential role in the transport of fluids across the cell plasma membrane. Recently, the expression of AQPs in dierent ocular tissues and their involvement in the pathophysiology of eye diseases, have garnered attention. Considering that literature on AQP expression in the lacrimal glands and their secretion is scarce, we aimed to characterise AQP1 expression in the tears of healthy dogs using two tear collection methods (Schirmer tear strips (STS) and ophthalmic sponges (OS)). Fifteen healthy dogs, free of ophthalmic diseases, were included in the study. Tear collection was performed by using STS in one eye and OS in the other. After the extraction of proteins from the tears, the expression of AQP1 was analysed by Western blotting. AQP1 was expressed as a band of 28 kDa. In addition, dierences were observed in the expression of AQP1 and in the correlation between tear volume and protein concentration, in tears collected by the two dierent methods. Our results suggest that AQP1 has a specific role in tear secretion; further research is required to assess its particular role in the function of the ocular surface in eye physiology and pathology

Lateral patellar luxation in nine small breed dogs

The objective of this paper was to describe the clinical features, the management and the outcome of nine small breed dogs affected with lateral patella luxation referred during the period between January 2010 and December 2014. Patellar luxations were classified according to: breed, age, sex, weight, and grade of patellar luxation, as well as if unilateral or bilateral, and concurrent cranial cruciate ligament lesion. In affected dogs, surgical correction consisted in the combination of tibial tuberosity transposition and soft tissue procedure. Adjunctive condroplasty or trochleoplasty was performed as needing. The outcome was found positive after surgical management with low complication rate and complications have been easily managed with high success rate

Traumatic lumbosacral joint dislocation in 3 dogs: Clinical presentation, diagnosis, treatment and short-term follow-up

Traumatic lumbosacral joint dislocation (TLSJD) without L7 vertebral body fracture is a rare injury in dogs. This report describes clinical presentation, therapy and outcome in 3 dogs. Three crossbreed dogs with non-ambulatory paraparesis and lumbar pain were referred. Radiographs confirmed TLSJD and dorso-ventral displacement (DVD) was measured pre and postoperatively. Case 1 was treated by percutaneous transilial pinning. Cases 2 and 3 were treated by internal fixation with pins and PMMA. Within 72h after surgery all dogs were able to stand and walk, and faecal incontinence resolved. To the author's knowledge this is the first description of a case series of TLSJD in the dog. The biomechanics of TLSJD in animals have not been investigated. It is likely that a single trauma severely hyper-extends L7-S1 causing disruption of the supra and inter-spinous ligaments with simultaneous shear and compression forces that cause ventral slipping of the sacrum. Pins and PMMA compared to percutaneous transilial pinning, provided more strength and stability. In conclusion, TLSJD requires appropriate surgical reduction and stabilization to allow fibrous healing of the L7-S1 junction, resulting in satisfactory neurological recovery

Biased total mass of cool core galaxy clusters by Sunyaev-Zel'dovich effect measurements

The Sunyaev Zel'dovich (SZ) effect from galaxy clusters is one of the most powerful cosmological tools for investigating the large-scale Universe. The big advantage of the SZ effect is its redshift independence, which is not the case for visible and X-ray observations. It allows us to directly estimate the cluster's total mass from the integrated comptonization parameter Y, even for distant clusters. However, not having a full knowing intra-cluster medium (ICM) physics can affect the results. By taking self-similar temperature and density profiles of the ICM into account, we studied how different ICM morphologies can affect the cluster total mass estimation. With the help of the high percentage of cool core (CC) clusters, as observed so far, the present analysis focuses on studying this class of objects. A sample of eight nearby (0.1 < z < 0.5) and high-mass (M > 10^(14) M_sun) clusters observed by Chandra was considered. We simulated SZ observations of these clusters through X-ray derived information and analyzed the mock SZ data again with the simplistic assumption of an isothermal beta-model profile for the ICM. The bias on the recovered cluster total mass using different sets of assumptions is estimated to be 50% higher in the case of hydrostatic equilibrium. Possible contributions to the total bias due to the line-of-sight integration and the considered ICM template are taken into account. The large biases on total mass recovery firmly support, if still necessary, cluster modeling based on more sophisticated universal profiles as derived by X-ray observations of local objects and hydrodynamical simulations.Comment: 11 pages, 4 figures; minor revisions. Accepted for publication in A&

Hourly energy profile determination technique from monthly energy bills

Hourly energy consumption profiles are of primary interest for measures to apply to the dynamics of the energy system. Indeed, during the planning phase, the required data availability and their quality is essential for a successful scenarios’ projection. As a matter of fact, the resolution of available data is not the requested one, especially in the field of their hourly distribution when the objective function is the production-demand matching for effective renewables integration. To fill this gap, there are several data analysis techniques but most of them require strong statistical skills and proper size of the original database. Referring to the built environment data, the monthly energy bills are the most common and easy to find source of data. This is why the authors in this paper propose, test and validate an expeditious mathematical method to extract the building energy demand on an hourly basis. A benchmark hourly profile is considered for a specific type of building, in this case an office one. The benchmark profile is used to normalize the consumption extracted from the 3 tariffs the bill is divided into, accounting for weekdays, Saturdays and Sundays. The calibration is carried out together with a sensitivity analysis of on-site solar electricity production. The method gives a predicted result with an average 25% MAPE and a 32% cvRMSE during one year of hourly profile reconstruction when compared with the measured data given by the Distributor System Operator (DSO)

Normal ultrasonographic features of loggerhead (Caretta caretta) eyes

The Loggerhead sea turtle (Caretta caretta) is widely distributed in the Mediterranean Sea but, like other sea turtles, it is considered a threatened species. Improving anatomical knowledge on normal aspects of the species is important for correct diagnosis and proper therapy and, therefore, for improving the chances of a complete recovery and a fast reintroduction into the wild for a greater number of sea turtle bycatch and other mishaps. For this reason, 15 Loggerhead turtles, with clinically healthy eyes, were submitted to ocular ultrasonographic (US) examinations. The US exam was quick, non-invasive, and simple to perform and permitted researchers to assess all the ocular features. © 2020, Croatian Natural History Museum. All rights reserved

Millimeter and sub-millimeter atmospheric performance at Dome C combining radiosoundings and ATM synthetic spectra

The reliability of astronomical observations at millimeter and sub-millimeter wavelengths closely depends on a low vertical content of water vapor as well as on high atmospheric emission stability. Although Concordia station at Dome C (Antarctica) enjoys good observing conditions in this atmospheric spectral windows, as shown by preliminary site-testing campaigns at different bands and in, not always, time overlapped periods, a dedicated instrument able to continuously determine atmospheric performance for a wide spectral range is not yet planned. In the absence of such measurements, in this paper we suggest a semi-empirical approach to perform an analysis of atmospheric transmission and emission at Dome C to compare the performance for 7 photometric bands ranging from 100 GHz to 2 THz. Radiosoundings data provided by the Routine Meteorological Observations (RMO) Research Project at Concordia station are corrected by temperature and humidity errors and dry biases and then employed to feed ATM (Atmospheric Transmission at Microwaves) code to generate synthetic spectra in the wide spectral range from 100 GHz to 2 THz. To quantify the atmospheric contribution in millimeter and sub-millimeter observations we are considering several photometric bands in which atmospheric quantities are integrated. The observational capabilities of this site at all the selected spectral bands are analyzed considering monthly averaged transmissions joined to the corresponding fluctuations. Transmission and pwv statistics at Dome C derived by our semi-empirical approach are consistent with previous works. It is evident the decreasing of the performance at high frequencies. We propose to introduce a new parameter to compare the quality of a site at different spectral bands, in terms of high transmission and emission stability, the Site Photometric Quality Factor.Comment: accepted to MNRAS with minor revision

SWIPE: a bolometric polarimeter for the Large-Scale Polarization Explorer

The balloon-borne LSPE mission is optimized to measure the linear polarization of the Cosmic Microwave Background at large angular scales. The Short Wavelength Instrument for the Polarization Explorer (SWIPE) is composed of 3 arrays of multi-mode bolometers cooled at 0.3K, with optical components and filters cryogenically cooled below 4K to reduce the background on the detectors. Polarimetry is achieved by means of large rotating half-wave plates and wire-grid polarizers in front of the arrays. The polarization modulator is the first component of the optical chain, reducing significantly the effect of instrumental polarization. In SWIPE we trade angular resolution for sensitivity. The diameter of the entrance pupil of the refractive telescope is 45 cm, while the field optics is optimized to collect tens of modes for each detector, thus boosting the absorbed power. This approach results in a FWHM resolution of 1.8, 1.5, 1.2 degrees at 95, 145, 245 GHz respectively. The expected performance of the three channels is limited by photon noise, resulting in a final sensitivity around 0.1-0.2 uK per beam, for a 13 days survey covering 25% of the sky.Comment: In press. Copyright 2012 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibite

Bistability in the Tunnelling Current through a Ring of NN Coupled Quantum Dots

We study bistability in the electron transport through a ring of N coupled quantum dots with two orbitals in each dot. One orbital is localized (called b orbital) and coupling of the b orbitals in any two dots is negligible; the other is delocalized in the plane of the ring (called d orbital), due to coupling of the d orbitals in the neighboring dots, as described by a tight-binding model. The d orbitals thereby form a band with finite width. The b and d orbitals are connected to the source and drain electrodes with a voltage bias V, allowing the electron tunnelling. Tunnelling current is calculated by using a nonequilibrium Green function method recently developed to treat nanostructures with multiple energy levels. We find a bistable effect in the tunnelling current as a function of bias V, when the size N>50; this effect scales with the size N and becomes sizable at N~100. The temperature effect on bistability is also discussed. In comparison, mean-field treatment tends to overestimate the bistable effect.Comment: Published in JPSJ; minor typos correcte

The optical design of the Litebird middle and high frequency telescope

LiteBIRD is a JAXA strategic L-class mission devoted to the measurement of polarization of the Cosmic Microwave Background, searching for the signature of primordial gravitational waves in the B-modes pattern of the polarization. The onboard instrumentation includes a Middle and High Frequency Telescope (MHFT), based on a pair of cryogenically cooled refractive telescopes covering, respectively, the 89-224 GHz and the 166-448 GHz bands. Given the high target sensitivity and the careful systematics control needed to achieve the scientific goals of the mission, optical modeling and characterization are performed with the aim to capture most of the physical effects potentially affecting the real performance of the two refractors. We describe the main features of the MHFT, its design drivers and the major challenges in system optimization and characterization. We provide the current status of the development of the optical system and we describe the current plan of activities related to optical performance simulation and validation