Study of the emergence and evolutionary dynamics of Armillaria ostoyae a pathogen of maritime pine

Dans la forêt de pin maritime (Pinus pinaster) des Landes de Gascogne (sud-ouest de France), la mortalité des pins causée par le champignon pourridié Armillaria ostoyae (Basidiomycète) a augmenté au cours des 30 dernières années. Les premiers cas de cette maladie ont été signalés quelques années après un changement majeur dans l'utilisation des terres, qui a eu lieu dans cette région suite au remplacement des landes et marais d'origine par une forêt plantée et gérée da façon intensive. Notre objectif était de comprendre les facteurs à l'origine de cette maladie émergente. Pour cela, nous avons étudié la distribution spatiale des dommages causés par le pathogène en relation avec des facteurs historiques, estimé la variabilité des traits fongiques liés au parasitisme et saprophytisme, et étudié l'histoire démographique d'A. ostoyae. La répartition actuelle de la mortalité induite par A. ostoyae est apparue dépendre de la présence des forêts préexistantes, ce qui suggère qu'A. ostoyae était fréquent dans ces zones forestières anciennes, qui ont agi comme un réservoir pour la colonisation des forêts plantées récentes. La production de rhizomorphes était significativement corrélée avec la virulence, suggérant que ce trait joue un rôle important dans le stade parasitaire d'A. ostoyae. Aucune relation significative entre le parasitisme et saprophytisme n'a été détectée, suggérant une absence de compromis évolutif entre ces traits. Enfin, le meilleur scénario démographique pour expliquer la structure de la population d'A. ostoyae dans la forêt des Landes est un scénario en deux étapes : il y aurait eu d'abord une diminution puis une expansion de la population fongique, qui semblait suivre la dynamique de la population d'hôtes. Le temps de génération d’A. ostoyae a été estimé entre 10 et 20 ans.In the maritime pine (Pinus pinaster) forest of the Landes de Gascogne (south-western France), pine mortality due to the root rot fungus Armillaria ostoyae (Basidiomycete) has been increasing over the last 30 years. The first cases of this disease were reported a few years after a major change in land use which occurred in this region following the replacement of original moors by an intensively managed planted forest. Our aim was to understand the factors driving this disease emergence. For this, we investigated the spatial distribution of pathogen damage related to historical factors, estimated the variation in fungal traits related to parasitism and saprophytism and investigated the demographic history of A. ostoyae. The current distribution of A. ostoyae mortality appeared depending on the pre-existing forests, suggesting that A. ostoyae was commonly distributed in pre-existing forest areas which acted as a reservoir for the colonization of recent planted forests. The rhizomorphs production was significantly correlated with virulence, suggesting that this trait plays an important role in the parasitic stage of A. ostoyae, but no significant relationship between parasitism and saprophytism components was detected, which may suggest that there is no trade-off between these traits. Finally, the best demographic scenario to explain A. ostoyae population structure in the Landes forest is a two step scenario: there was first a decrease and then an expansion in the fungal population, which appeared to follow the dynamics of the host population. The generation time of A. ostoyae was estimated between 10 and 20 years

Etude des lettres d'admission aux urgences pédiatriques de Clermont-Ferrand

CLERMONT FD-BCIU-Santé (631132104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Etude des lettres d'admission aux urgences pédiatriques de Clermont-Ferrand

CLERMONT FD-BCIU-Santé (631132104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Genetic structure and diversity of Armillaria ostoyae, agent of an emerging fungal disease in the recent south-western French maritime pine forest

National audienc

Genetic analysis reveals efficient sexual spore dispersal at a fine spatial scale in Armillaria ostoyae, the causal agent of root-rot disease in conifers

Armillaria ostoyae (sometimes named Armillaria solidipes) is a fungal species causing root diseases in numerous coniferous forests of the northern hemisphere. The importance of sexual spores for the establishment of new disease centres remains unclear, particularly in the large maritime pine plantations of southwestern France. An analysis of the genetic diversity of a local fungal population distributed over 500 ha in this French forest showed genetic recombination between genotypes to be frequent, consistent with regular sexual reproduction within the population. The estimated spatial genetic structure displayed a significant pattern of isolation by distance, consistent with the dispersal of sexual spores mostly at the spatial scale studied. Using these genetic data, we inferred an effective density of reproductive individuals of 0.1-0.3 individuals/ha, and a second moment of parent-progeny dispersal distance of 130-800 m, compatible with the main models of fungal spore dispersal. These results contrast with those obtained for studies of A. ostoyae over larger spatial scales, suggesting that inferences about mean spore dispersal may be best performed at fine spatial scales (i.e. a few kilometres) for most fungal species

Genetic signature of a range expansion and leap-frog event after the recent invasion of Europe by the grapevine downy mildew pathogen Plasmopara viticola

International audienc

Variation in traits associated with parasitism and saprotrophism in a fungal root-rot pathogen invading intensive pine plantations

Armillaria ostoyae, the causal agent of root- and butt-rot in several forest trees, has a lifecycle consisting of alternating parasitic and saprotrophic stages. It causes high levels of mortality in the intensively managed monospecific plantations of maritime pine (Pinus pinaster) in south-western France (Landes forest). In this region, the pathogen was native to the forests, pre-dating the large plantations of the 19th century. The first objective of this study was to estimate the variation in aggressiveness on maritime pine, determined as rate of host mortality caused by the infection process. The second objective was to characterize relationships between aggressiveness and traits likely to be involved in parasitism (i.e. rhizomorph production and colonization of host tissues) and saprotrophism (ability to decompose wood). The A. ostoyae isolates studied caused high rates of mortality in maritime pine, with significant differences between isolates. However, there was no variation of aggressiveness between A. ostoyae isolates from ancient forested and from more recently afforested areas, and did not support the hypothesis of a higher aggressiveness linked to a recent range expansion and the intensification of silviculture in this area. Rhizomorph production and aggressiveness were significantly correlated. In addition, we did not detect any trade-off between components of parasitism and saprotrophism, suggesting no significant evolutionary constraint driving these traits

Insecticide resistance in disease vectors from Mayotte : an opportunity for integrated vector management

Background: Mayotte, a small island in the Indian Ocean, has been affected for many years by vector-borne diseases. Malaria, Bancroftian filariasis, dengue, chikungunya and Rift Valley fever have circulated or still circulate on the island. They are all transmitted by Culicidae mosquitoes. To limit the impact of these diseases on human health, vector control has been implemented for more than 60 years on Mayotte. In this study, we assessed the resistance levels of four major vector species (Anopheles gambiae, Culex pipiens quinquefasciatus, Aedes aegypti and Aedes albopictus) to two types of insecticides: i) the locally currently-used insecticides (organophosphates, pyrethroids) and ii) alternative molecules that are promising for vector control and come from different insecticide families (bacterial toxins or insect growth regulators). When some resistance was found to one of these insecticides, we characterized the mechanisms involved. Methods: Larval and adult bioassays were used to evaluate the level of resistance. When resistance was found, we tested for the presence of metabolic resistance through detoxifying enzyme activity assays, or for target-site mutations through molecular identification of known resistance alleles. Results: Resistance to currently-used insecticides varied greatly between the four vector species. While no resistance to any insecticides was found in the two Aedes species, bioassays confirmed multiple resistance in Cx. p. quinquefasciatus (temephos: similar to 20 fold and deltamethrin: only 10% mortality after 24 hours). In An. gambiae, resistance was scarce: only a moderate resistance to temephos was found (similar to 5 fold). This resistance appears to be due only to carboxyl-esterase overexpression and not to target modification. Finally, and comfortingly, none of the four species showed resistance to any of the new insecticides. Conclusions: The low resistance observed in Mayotte's main disease vectors is particularly interesting, because it leaves a range of tools useable by vector control services. Together with the relative isolation of the island (thus limited immigration of mosquitoes), it provides us with a unique place to implement an integrated vector management plan, including all the good practices learned from previous experiences