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Identification of a carbohydrate-based endothelial ligand for a lymphocyte homing receptor.
Lymphocyte attachment to high endothelial venules within lymph nodes is mediated by the peripheral lymph node homing receptor (pnHR), originally defined on mouse lymphocytes by the MEL-14 mAb. The pnHR is a calcium-dependent lectin-like receptor, a member of the LEC-CAM family of adhesion proteins. Here, using a soluble recombinant form of the homing receptor, we have identified an endothelial ligand for the pnHR as an approximately 50-kD sulfated, fucosylated, and sialylated glycoprotein, which we designate Sgp50 (sulfated glycoprotein of 50 kD). Recombinant receptor binding to this lymph node-specific glycoprotein requires calcium and is inhibitable by specific carbohydrates and by MEL-14 mAb. Sialylation of the component is required for binding. Additionally, the glycoprotein is precipitated by MECA-79, an adhesion-blocking mAb reactive with lymph node HEV. A related glycoprotein of approximately 90 kD (designated as Sgp90) is also identified
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The complement binding-like domains of the murine homing receptor facilitate lectin activity.
The leukocyte homing receptor (HR), the endothelial leukocyte adhesion molecule, and gmp140/platelet activation-dependent granule membrane protein are members of a family of adhesion molecules, termed the lectin cell adhesion molecules (LEC-CAMS) which are unified by a multi-domain structure containing a lectin motif, an epidermal growth factor-like (egf) motif, and variable numbers of a complement binding-like (CB) motif. Previous data have indicated a predominant role for the lectin motif in cell adhesion directed by the LEC-CAMS, although the egf-like domain of the HR may also play a potential role in cell binding. While the role(s) of the CB domains in the LEC-CAMS is currently not understood, they have been hypothesized to act as rigid spacers or stalks for lectin and perhaps, egf domain presentation. In this paper, we analyze the functional characteristics of murine HR-IgG chimeras containing the lectin, lectin plus egf, and lectin plus egf plus CB domains. The Mel 14 mAb, an adhesion blocking antibody which recognizes a conformational determinant in the N-terminus of the HR lectin domain, shows a significantly decreased affinity for a HR construct which lacks the CB motifs, consistent with the possibility that the CB domains are involved with lectin domain structure. In agreement with this conjecture, HR mutants lacking the CB domains show a profound decrease in lectin-specific interaction with the carbohydrate polyphosphomannan ester, suggesting that the changes in Mel 14 affinity for the lectin domain are reflected in lectin functionality. Various assays investigating the interactions between the HR deletion mutants and the peripheral lymph node high endothelium, including cell blocking, immunohistochemical staining, and radioactively labeled ligand binding, all showed that removal of the CB domains results in a lack of HR adhesive function. These results imply that the CB domains of the HR, and, by analogy, the other members of the LEC-CAM family, may play important structural roles involving induction of lectin domain conformation and resultant functionality
Effect of venting range hood flow rate on size-resolved ultrafine particle concentrations from gas stove cooking
Cooking is the main source of ultrafine particles (UFP) in homes. This study investigated the effect of venting range hood flow rate on size-resolved UFP concentrations from gas stove cooking. The same cooking protocol was conducted 60 times using three venting range hoods operated at six flow rates in twin research houses. Size-resolved particle (10–420 nm) concentrations were monitored using a NanoScan scanning mobility particle sizer (SMPS) from 15 min before cooking to 3 h after the cooking had stopped. Cooking increased the background total UFP number concentrations to 1.3 × 103 particles/cm3 on average, with a mean exposure-relevant source strength of 1.8 × 1012 particles/min. Total particle peak reductions ranged from 25% at the lowest fan flow rate of 36 L/s to 98% at the highest rate of 146 L/s. During the operation of a venting range hood, particle removal by deposition was less significant compared to the increasing air exchange rate driven by exhaust ventilation. Exposure to total particles due to cooking varied from 0.9 to 5.8 × 104 particles/cm3·h, 3 h after cooking ended. Compared to the 36 L/s range hood, higher flow rates of 120 and 146 L/s reduced the first-hour post-cooking exposure by 76% and 85%, respectively. © 2018 Crown Copyright. Published with license by Taylor & Francis Group, LLC
The Dilaton Theorem and Closed String Backgrounds
The zero-momentum ghost-dilaton is a non-primary BRST physical state present
in every bosonic closed string background. It is given by the action of the
BRST operator on another state \x, but remains nontrivial in the semirelative
BRST cohomology. When local coordinates arise from metrics we show that dilaton
and \x insertions compute Riemannian curvature and geodesic curvature
respectively. A proper definition of a CFT deformation induced by the dilaton
requires surface integrals of the dilaton and line integrals of \x.
Surprisingly, the ghost number anomaly makes this a trivial deformation. While
dilatons cannot deform conformal theories, they actually deform conformal
string backgrounds, showing in a simple context that a string background is not
necessarily the same as a CFT. We generalize the earlier proof of quantum
background independence of string theory to show that a dilaton shift amounts
to a shift of the string coupling in the field-dependent part of the quantum
string action. Thus the ``dilaton theorem'', familiar for on-shell string
amplitudes, holds off-shell as a consequence of an exact symmetry of the string
action.Comment: 51 pages, plain tex with phyzzx, two uuencoded figure
Response of Spiking Neurons to Correlated Inputs
The effect of a temporally correlated afferent current on the firing rate of
a leaky integrate-and-fire (LIF) neuron is studied. This current is
characterized in terms of rates, auto and cross-correlations, and correlation
time scale of excitatory and inhibitory inputs. The output rate
is calculated in the Fokker-Planck (FP) formalism in the limit of
both small and large compared to the membrane time constant of
the neuron. By simulations we check the analytical results, provide an
interpolation valid for all and study the neuron's response to rapid
changes in the correlation magnitude.Comment: 4 pages, 3 figure
Spin susceptibility, phase diagram, and quantum criticality in the electron-doped high Tc Superconductor Ba[Fe(1-x)Co(x)]2As2
We report a systematic investigation of Ba[Fe(1-x)Co(x)]2As2 based on
transport and 75-As NMR measurements, and establish the electronic phase
diagram. We demonstrate that doping progressively suppresses the uniform spin
susceptibility and low frequency spin fluctuations. The optimum superconducting
phase emerges at x_c~0.08 when the tendency toward spin ordering completely
diminishes. Our findings point toward the presence of a quantum critical point
near x_c between the SDW (spin density wave) and superconducting phases.Comment: 5 Figure
Efficient Bayesian-based Multi-View Deconvolution
Light sheet fluorescence microscopy is able to image large specimen with high
resolution by imaging the sam- ples from multiple angles. Multi-view
deconvolution can significantly improve the resolution and contrast of the
images, but its application has been limited due to the large size of the
datasets. Here we present a Bayesian- based derivation of multi-view
deconvolution that drastically improves the convergence time and provide a fast
implementation utilizing graphics hardware.Comment: 48 pages, 20 figures, 1 table, under review at Nature Method
Perioperative treatment with phosphatidic acid inhibitor (Lisofylline) leads to prolonged survival of hearts in the guinea pig to rat xenotransplant model
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