Conjugated Linoleic Acid Supplementation: Lipid Content And Hepatic Histology In Healthy Wistar Rats [suplementação Com ácido Linoleico Conjugado: Conteúdo De Lípides E Histologia Hepática De Ratos Wistar Saudáveis]

This work aimed to evaluate the effects of the consumption of two commercial conjugated linoleic acid (CLA) mixtures on lipid content and liver histology of healthy rats. The investigation was carried out using thirty rats divided into three groups: C (control), AE (AdvantEdge®CLA), and CO (CLA One®). The concentration of CLA was 2% of feed consumption, and the animals were supplemented daily for 42 days. The total lipid content of the liver was determined, and the histology of the organ was examined by Transmission Electronic Microscopy. The results of total liver lipid contents did not exhibit significant differences between the groups. With regard to hepatic histology, it was observed that although fat globules were visibly present in higher numbers and bigger size in the CLA groups, the organ histology was considered normal since both cytoplasm and organelles showed integrity. It was concluded that even though liver microscopic images indicated the presence of fat globules in the liver, from a statistical point of view, the supplementation for 42 days did not bring about lipid accumulation, nor did it alter hepatic histology.311141146Adams, L.A., Angulo, P., Lindor, K.D., Nonalcoholic fatty liver disease (2005) Canadian Medical Association Journal, 172 (7), pp. 899-905Akbiyik, F., Ligand-induced expression of peroxissome proliferators activated receptor α and activation of fatty oxidation enzymes in fatty liver (2004) European Journal of Clinical Investigation, 34 (6), pp. 429-435Akyüz, F., What is the best indicator for evaluating treatment response in nonalcoholic fatty liver disease: Histology or aminotransferase levels? 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Anuran Species Of The Salto Morato Nature Reserve In Paraná, Southern Brazil: Review Of The Species List

We provide an updated checklist of the anurans of the Salto Morato Nature Reserve (SMNR), Paraná, southern Brazil, including information on species endemism, conservation status, habitat use, and reproductive modes. We sampled the study area between February 2013 and July 2015, using Visual Encounter Surveys, totaling 700 hours of sampling effort. We supplement our primary data with secondary data (published papers and voucher specimens), and through these, we found a total of 54 anuran species representing 23 genera and 10 families. All the frogs recorded are endemic to the Atlantic Forest. Although no species has been listed as Endangered on the Red Lists of Paraná state, Brazil, and the IUCN, five are classified as Data Deficient. Most of the species recorded were arboreal (55.5%), a third were terrestrial (33.3%), and the remainders were rheophilic (11.1%). We identified 18 different reproductive modes, with types 1 (lay eggs in lentic water where the tadpoles develop) and 23 (direct development of terrestrial eggs) being the most common. Our findings indicate that the SMNR currently has the greatest diversity of anuran species and reproductive modes of any area in southern Brazil. © 2016 Check List and Authors.12

Water requirements and footprint of a super intensive olive grove under Mediterranean climate

Abstract The water footprint of a product can be described as the volume of freshwater used to produce it, associated to a geographic and temporal resolution. For crops, the water footprint relates crop water requirements and yield. The components of water footprint, blue, green and grey water footprints, refer to the volumes of respectively, surface and groundwater, rainfall, and water required to assimilate pollution, used to produce the crop yield. The global standard for crop water footprint assessment relies on evapotranspiration models to estimate green and blue water evapotranspiration. This approach has been used in the present study to estimate the water footprint of a very high density drip irrigated olive grove and further compared with data obtained from evapotranspiration measurements or from its components: the eddy covariance method to quantify latent heat flux, a heat dissipation sap flow technique to determine transpiration and microlysimeters to evaluate soil evaporation. The eddy covariance technique was used for short periods in 2011 and 2012, while sap flow measurements were performed continuously, hence allowing the extension of the data series. Measurements of evapotranspiration with the eddy covariance method provided an average close to 3.4 mm d-1 (2011) and 2.5 mm d-1 (2012). The ratio of evapotranspiration to reference evapotranspiration approached 0.6 and 0.4 for the respective periods. The water footprint of the olive crop under study, calculated with field data, was higher than the water footprint simulated using the global standard assessment and was lower than that reported in literature for olives. Lower values are probably related to differences in cultural practices, e.g., the density of plantation, harvesting techniques and irrigation management. The irrigated high-density olive grove under study had a high yield, which compensates for high water consumption, thus leading to a water footprint lower than the ones of rainfed or less dense groves. Other differences may relate to the procedures used to determine evapotranspiration