11 research outputs found
Genome-inspired molecular identification in organic matter via Raman spectroscopy
Rapid, non-destructive characterization of molecular level chemistry for
organic matter (OM) is experimentally challenging. Raman spectroscopy is one of
the most widely used techniques for non-destructive chemical characterization,
although it currently does not provide detailed identification of molecular
components in OM, due to the combination of diffraction-limited spatial
resolution and poor applicability of peak-fitting algorithms. Here, we develop
a genome-inspired collective molecular structure fingerprinting approach, which
utilizes ab initio calculations and data mining techniques to extract molecular
level chemistry from the Raman spectra of OM. We illustrate the power of such
an approach by identifying representative molecular fingerprints in OM, for
which the molecular chemistry is to date inaccessible using non-destructive
characterization techniques. Chemical properties such as aromatic cluster size
distribution and H/C ratio can now be quantified directly using the identified
molecular fingerprints. Our approach will enable non-destructive identification
of chemical signatures with their correlation to the preservation of
biosignatures in OM, accurate detection and quantification of environmental
contamination, as well as objective assessment of OM with respect to their
chemical contents
Hydration behavior by X-ray diffraction profile fitting of smectite-bearing minerals
Clay mineral hydration and dehydration processes are reversible at temperatures <100 °C and strongly affect wellbore stability, fines migration, permeability, and dispersion of pore pressure. The hydration behavior of smectite-rich material as a function of relative humidity (activity of water, aw, controlled by salinity) and temperature was studied using in situ X-ray diffraction on a material retrieved from coring in the Gulf of Mexico. X-ray diffraction profile fitting was used to explore the competition for water between hydratable phases across a range of relative humidity, 2 % to 90 %, and temperature, 25°C to 95°C, conditions. X-ray diffraction profile fitting employed a modified multi-specimen approach in which proportions of minerals were modelled using Ca-exchanged preparations in air-dried and ethylene glycol solvated states. Across the range of hydration states, the mineral proportions and crystallographic parameters remained constant from the multi-specimen approach and only the number of water layers in hydratable phases varied. Quantitative clay mineralogy showed a natural material with a discrete smectite component and a mixed-layered illite-smectite, both capable of hydration/dehydration. Results of this study showed the discrete smectite component and the mixed-layered illite-smectite hydrated at different rates with discrete smectite up-taking more water at lower relative humidity than the mixed-layered illite-smectite. Over geological time this study highlights the non-static nature of smectite hydration with implications of long-term creep and permeability behavior
Recommended from our members
The Relative Abundances of Resolved 12CH2D2 and 13CH3D and Mechanisms Controlling Isotopic Bond Ordering in Abiotic and Biotic Methane Gases
We report measurements of resolved 12CH2D2 and 13CH3D at natural abundances in a variety of methane gases produced naturally and in the laboratory. The ability to resolve 12CH2D2 from 13CH3D provides unprecedented insights into the origin and evolution of CH4. The results identify conditions under which either isotopic bond order disequilibrium or equilibrium are expected. Where equilibrium obtains, concordant Î12CH2D2 and Î13CH3D temperatures can be used reliably for thermometry. We find that concordant temperatures do not always match previous hypotheses based on indirect estimates of temperature of formation nor temperatures derived from CH4/H2 D/H exchange, underscoring the importance of reliable thermometry based on the CH4 molecules themselves. Where Î12CH2D2 and Î13CH3D values are inconsistent with thermodynamic equilibrium, temperatures of formation derived from these species are spurious. In such situations, while formation temperatures are unavailable, disequilibrium isotopologue ratios nonetheless provide novel information about the formation mechanism of the gas and the presence or absence of multiple sources or sinks. In particular, disequilibrium isotopologue ratios may provide the means for differentiating between methane produced by abiotic synthesis vs. biological processes. Deficits in 12CH2D2 compared with equilibrium values in CH4 gas made by surface-catalyzed abiotic reactions are so large as to point towards a quantum tunneling origin. Tunneling also accounts for the more moderate depletions in 13CH3D that accompany the low 12CH2D2 abundances produced by abiotic reactions. The tunneling signature may prove to be an important tracer of abiotic methane formation, especially where it is preserved by dissolution of gas in cool hydrothermal systems (e.g., Mars). Isotopologue signatures of abiotic methane production can be erased by infiltration of microbial communities, and Î12CH2D2 values are a key tracer of microbial recycling