A study of psychrophilic organisms isolated from the manufacture and assembly areas of spacecraft to be used in the Viking mission

The effect of storage of dry heat treated Teflon ribbons under nitrogen gas followed by high vacuum on the recovery of hardy organisms from the ribbons was studied. A similar experiment was performed on spore crops of hardy organisms recovered previously from Cape Canaveral. Hardy organisms have been inoculated onto slides and subjected to an artificial Martian environment in an attempt to demonstrate their growth in this environment. Additional experiments using the artificial Martian environment include response of soil samples from the VAB with both constant temperature and freeze-thaw cycles. These experiments were performed with dried soil and soil containing added water. Other investigations included the effect of heatshock on soil samples, psychrophilic counts of new soil samples from the manufacture area of the Viking spacecraft, effect of pour plate versus spread plate on psychrophilic counts, and preparation of spore crops of hardy organisms from Cape Canaveral

Response of selected microoganisms to experimental planetary environments

A microbial population profile of mixed Cape Canaveral soil samples is presented. During this investigation a few organisms were isolated which exhibit the ability to grow at 3 C, 32 C, and 55 C. Growth curves are shown for three of these isolates, one of which grows extremely well at all three temperatures. Also included are studies dealing with growth of soil populations at zero and subzero temperatures. Results indicate growth at 0 C and -5 C, but not at 15 C or -65 C. The effect of storage temperature on dry soil is presented, and results show that psychrophilic populations decrease when soil is stored at room temperature, but do not decrease when soil is stored at -65 C. Results of an experiment with the simulated Martian environment are presented and indicate that nonsporeforming rods, sporeforming rods, and cocci can reproduce in the simulated environment when nutrients and moisture are supplied. The sporeforming rods are the predominant suvivors when dry soil is subjected to this environment

A study of psychrophilic organisms isolated from the manufacture and assembly areas of spacecraft to be used in the Viking mission

The ability of psychrophilic microorganisms to grow in some of the environmental conditions suggested for Mars is studied with particular attention given to the effects of moisture and nutrients on growth. Results of growth with the slide culture technique are presented and indicate that this technique can be a rapid and sensitive technique for demonstration of microbial growth under various environmental conditions. Additional soil samples have been obtained from Cape Kennedy, and results of these assays at various low temperatures for psychrophilic populations are presented. The heat resistance of some of the psychrophilic sporeformers have been determined. Psychrophilic organisms were isolated from the teflon ribbons at Cape Kennedy and characterization of these was begun. In addition, heat survivors from the teflon ribbons are being investigated, and partial characterizations of these are presented

Response of Selected Microorganisms to Experimental Planetary Environments

Results of studies in anaerobic phosphorus metabolism are presented. Specific topics discussed include: (1) anaerobic utilization of PH3; (2) reduction of phosphate or phosphite; (3) isolation of organisms which utilize phosphite or phosphate anaerobically as a final hydrogen acceptor; and (4) the toxicity of PH3 to the organisms. Techniques of anaerobic microbiology associated with space hardware were also studied. These include: (1) the Brewer anaerobe jar/GasPak system; (2) a new procedure to grow aerobes and anaerobes simultaneously; (3) a culture medium to differentiate oblagate from facultative anaerobes; and (4) a procedure to quantitate O2 sensitivity of anaerobes

The Connection between Supernova Remnants and the Galactic Magnetic Field: A Global Radio Study of the Axisymmetric Sample

The study of supernova remnants (SNRs) is fundamental to understanding the chemical enrichment and magnetism in galaxies, including our own Milky Way. In an effort to understand the connection between the morphology of SNRs and the Galactic magnetic field (GMF), we have examined the radio images of all known SNRs in our Galaxy and compiled a large sample that have an "axisymmetric" morphology, which we define to mean SNRs with a "bilateral" or "barrel"-shaped morphology, in addition to one-sided shells. We selected the cleanest examples and model each of these at their appropriate Galactic position using two GMF models, those of Jansson & Farrar (2012a), which includes a vertical halo component, and Sun et al. (2008) that is oriented entirely parallel to the plane. Since the magnitude and relative orientation of the magnetic field changes with distance from the sun, we analyse a range of distances, from 0.5 to 10 kpc in each case. Using a physically motivated model of a SNR expanding into the ambient GMF, we find the models using Jansson & Farrar (2012a) are able to reproduce observed morphologies of many SNRs in our sample. These results strongly support the presence of an off-plane, vertical component to the GMF, and the importance of the Galactic field on SNR morphology. Our approach also provides a potential new method for determining distances to SNRs, or conversely, distances to features in the large-scale GMF if SNR distances are known.Comment: 24 pages, 8 figures plus one 5-page appendix figure, 3 tables, accepted to A&

Shuttle system ascent aerodynamic and plume heating

The shuttle program provided a challenge to the aerothermodynamicist due to the complexity of the flow field around the vehicle during ascent, since the configuration causes multiple shock interactions between the elements. Wind tunnel tests provided data for the prediction of the ascent design heating environment which involves both plume and aerodynamic heating phenomena. The approach for the heating methodology based on ground test firings and the use of the wind tunnel data to formulate the math models is discussed

The matching law

This article introduces the quantitative analysis of choice behavior by describing a number of equations developed over the years to describe the relation between the allocation of behavior under concurrent schedules of reinforcement and the consequences received for alternative responses. Direct proportionality between rate of responding and rate of reinforcement was observed in early studies, suggesting that behavioral output matched environmental input in a mathematical sense. This relation is termed "strict matching," and the equation that describes it is referred to as "the matching law." Later data showed systematic departures from strict matching, and a generalized version of the matching equation is now used to describe such data. This equation, referred to as "the generalized matching equation," also describes data that follow strict matching. It has become convention to refer to either of these equations as "the matching law." Empirical support for the matching law is briefly summarized, as is the applied and practical significance of matching analyses

‘I’m not a natural mathematician’: Inquiry-based learning, constructive alignment and introductory quantitative social science

There is continuing concern about the paucity of social science graduates who have the quantitative skills required by academia and industry. Not only do students often lack the confidence to explore, and use, statistical techniques, the dominance of qualitative research in many disciplines has also often constrained programme-level integration of more quantitative material. However, whilst the topic of statistical literacy is relatively well researched within the more general educational literature, the evidence-base with respect to the effectiveness of teaching and learning of quantitative research methods in the social science remains somewhat limited. This paper describes the development, integration and evaluation of a series of student-led inquiry-based quantitative workbooks within a sociology/social policy undergraduate degree. It outlines how the workbooks were constructively aligned within a ‘methods spine’ and offers some insight into quantitative teaching and learning generally. The paper also discusses some of the opportunities and challenges of taking both an aligned and IBL approach to the teaching of quantitative methods. In doing so it adds to growing evidence that ‘problem-based pedagogies’ tend to increase educational gain over and above more didactic approaches to learning and teaching. It highlights three key findings: programme-level approaches to curriculum design can be crucial in improving quantitative skills, particularly where they are tailored to student needs; a general indifference to quantitative methods is likely to be due to a process of disenfranchisement that happens before and during students’ engagement with university; and, meaningfully engaging students as partners in the process of designing, integrating and evaluating curricula can help to overcome some of the barriers associated with the learning and teaching of quantitative skills

Response of selected microorganisms to experimental planetary environments

Anaerobic and aerobic sporeformers and non-sporeformers were cultivated anaerobically in nutrient media under various pressures (up to 1800 psi) of pure H2, CH4, NH3, and H2S. Viability assays were performed periodically to determine growth, survival, or spore survival. Hydrogen up to 1800 psi demonstrated little or no suppression of growth with the possible exception of Bacillus coagulans at 1800 psi. The obligate anaerobes grew very well. Under CH4 the obligate anaerobes again exhibited the most prolific growth, whereas the facultative anaerobes grew well except under higher pressures. Ammonia at low pressure was extremely toxic to all test organisms. At 100 psi all populations were killed within 24 hours except Staphylococcus aureus which survived for 72 hours and the Bacillus spp. which produced a surviving population of approximately 10,000 spores/ml. All populations in H2S were killed within 24 to 48 hours except Proteus mirabilis which decreased to 100 cells/ml and the Bacillus spp. Spore survival studies of two months duration demonstrated that B. coagulans and B. pumilus survived under all experimental conditions. Clostridium novyi type B and C. sporogenes were killed rapidly in NH3 and H2S and demonstrated no sporulation

Passive microwave applications to snowpack monitoring using satellite data

Nimbus-5 Electrically Scanned Microwave Radiometer data were analyzed for the fall of 1975 and winter and summer of 1976 over the Arctic Coastal Plain of Alaska to determine the applicability of those data to snowpack monitoring. It was found that when the snow depth remained constant at 12.7 cm, the brightness temperatures T sub B varied with air temperature. During April and May the production of ice lenses and layers within the snow, and possibly wet ground beneath the snow contribute to the T sub B variations also. Comparison of March T sub B values of three areas with the same (12.7 cm) snow depth showed that air temperature is the predominant factor controlling the T sub B differences among the three areas, but underlying surface conditions and individual snowpack characteristics are also significant factors