128 research outputs found
Cyclic nucleotides in tissues during long-term hypokinesia
Male Wistar rates were kept hypokinetic by placing them in small containers for 22 days. Blood plasma cAMP content was subsequently found increased, and cGMP content decreased, in the experimental animals. Liver and thymus cAMP content was similar in the control and experimental animals. There was a 20 and 38% decrease of cAMP content in the kidneys and spleen, respectively. Hypokinesia's reduction of cyclic nucleotides seems to inhibit RNA and protein synthesis
ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠ΅ ΡΡΠΏΠ΅ΡΠ½ΠΎΠΉ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΊΠΈ Ρ ΡΡΠΆΠ΅Π»ΠΎΠΉ Π΄ΠΈΡΠΏΠ»Π°Π·ΠΈΠ΅ΠΉ Π²ΡΠ»ΡΠ²Ρ
A case of successful photodynamic therapy (PDT) of the patient with grade 3 vulvar dysplasia. One course of treatment with photolon was performed. The photosensitizer was administered intravenously as 30 min infusion in dose of 1.0 mg/kg body weight. The PDT session was performed 3h after infusion of photolon, the light dose was 350 J/cm2. The treatment was well tolerated. Due to PDT a complete tumor regression was achieved. The patient received a strict follow-up schedule, for 8 months after PDT no recurrence was observed.Β ΠΠΏΠΈΡΠ°Π½ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ»ΡΡΠ°ΠΉ ΡΡΠΏΠ΅ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ (Π€ΠΠ’) Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ Π±ΠΎΠ»ΡΠ½ΠΎΠΉ Ρ Π΄ΠΈΡΠΏΠ»Π°Π·ΠΈΠ΅ΠΉ III ΡΡ. Π²ΡΠ»ΡΠ²Ρ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ 1 ΠΊΡΡΡ Π»Π΅ΡΠ΅Π½ΠΈΡ Ρ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠΌ ΡΠΎΡΠΎΠ»ΠΎΠ½. Π€ΠΎΡΠΎΡΠ΅Π½ΡΠΈΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΎΡ Π²Π²ΠΎΠ΄ΠΈΠ»ΠΈ Π²Π½ΡΡΡΠΈΠ²Π΅Π½Π½ΠΎ ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²ΠΎΠΌ 30-ΠΌΠΈΠ½ΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΡΠ·ΠΈΠΈ Π² Π΄ΠΎΠ·Π΅ 1,0 ΠΌΠ³/ΠΊΠ³ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π°. Π‘Π΅Π°Π½Ρ Π€ΠΠ’ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΠ΅ΡΠ΅Π· 3 Ρ ΠΏΠΎΡΠ»Π΅ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ ΡΠΎΡΠΎΠ»ΠΎΠ½Π°, ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΡ ΡΠ½Π΅ΡΠ³ΠΈΠΈ Π»Π°Π·Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±Π»ΡΡΠ΅Π½ΠΈΡ β 350 ΠΠΆ/ΡΠΌ2. ΠΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΊΠ° ΠΏΠ΅ΡΠ΅Π½Π΅ΡΠ»Π° ΡΠ΄ΠΎΠ²Π»Π΅ΡΠ²ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π€ΠΠ’ ΠΏΠΎΠ»ΡΡΠ΅Π½Π° ΠΏΠΎΠ»Π½Π°Ρ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ. ΠΠΎΡΠ»Π΅ Π»Π΅ΡΠ΅Π½ΠΈΡ Π±ΠΎΠ»ΡΠ½Π°Ρ Π½Π°Ρ
ΠΎΠ΄ΠΈΠ»Π°ΡΡ ΠΏΠΎΠ΄ ΡΡΡΠΎΠ³ΠΈΠΌ Π΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠ΅ΠΌ, Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 8 ΠΌΠ΅ΡΡΡΠ΅Π² Ρ ΠΌΠΎΠΌΠ΅Π½ΡΠ° ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ Π€ΠΠ’ β Π±Π΅Π· ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π° Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ.
Π€ΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅ΡΠ°ΠΏΠΈΡ Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅
The review is on opportunities and possibilities of application of photodynamic therapy in clinical practice. The advantages of this method are the targeting of effect on tumor foci and high efficiency along with low systemic toxicity. The results of the set of recent Russian and foreign clinical trials are represented in the review. The method is successfully used in clinical practice with both radical (for early vulvar, cervical cancer and pre-cancer, central early lung cancer, esophageal and gastric cancer, bladder cancer and other types of malignant tumors), and palliative care (including tumor pleuritis, gastrointestinal tumors and others). Photodynamic therapy delivers results which are not available for other methods of cancer therapy. Thus, photodynamic therapy allows to avoid gross scars (that is very important, for example, in gynecology for treatment of patients of reproductive age with cervical and vulvar cancer), delivers good cosmetic effect for skin tumors, allows minimal trauma for intact tissue surrounding tumor. Photodynamic therapy is also used in other fields of medicine, such as otorhinolaryngology, dermatology, ophthalmology, orthopaedics, for treatment of papilloma virus infection and purulent wounds as antibacterial therapy.ΠΠ±Π·ΠΎΡ ΠΏΠΎΡΠ²ΡΡΠ΅Π½ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π°ΠΌ ΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅. ΠΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π°ΠΌΠΈ Π΄Π°Π½Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° ΡΠ²Π»ΡΡΡΡΡ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΡΡΡ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π½Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠ΅ ΠΎΡΠ°Π³ΠΈ ΠΈ Π²ΡΡΠΎΠΊΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΡΠΈ Π½ΠΈΠ·ΠΊΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΠ½ΠΎΠΉ ΡΠΎΠΊΡΠΈΡΠ½ΠΎΡΡΠΈ. Π ΠΎΠ±Π·ΠΎΡΠ΅ ΠΏΡΠΈΠ²Π΅Π΄Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΡΠ΄Π° ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΈ Π·Π°ΡΡΠ±Π΅ΠΆΠ½ΡΡ
ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΠΎΡΠ»Π΅Π΄Π½ΠΈΡ
Π»Π΅Ρ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΌΠ΅ΡΠΎΠ΄ ΡΡΠΏΠ΅ΡΠ½ΠΎ ΠΏΡΠΈΠΌΠ΅Π½ΡΠ΅ΡΡΡ Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΊΠ°ΠΊ Ρ ΡΠ°Π΄ΠΈΠΊΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ΅Π»ΡΡ (ΠΏΡΠΈ ΡΠ°Π½Π½ΠΈΡ
ΡΡΠ°Π΄ΠΈΡΡ
ΡΠ°ΠΊΠ° ΠΈ ΠΏΡΠ΅Π΄ΡΠ°ΠΊΠ΅ Π²ΡΠ»ΡΠ²Ρ, ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ, ΡΠ°Π½Π½Π΅ΠΌ ΡΠ΅Π½ΡΡΠ°Π»ΡΠ½ΠΎΠΌ ΡΠ°ΠΊΠ΅ Π»Π΅Π³ΠΊΠΈΡ
, ΡΠ°ΠΊΠ΅ ΠΏΠΈΡΠ΅Π²ΠΎΠ΄Π° ΠΈ ΠΆΠ΅Π»ΡΠ΄ΠΊΠ°, ΡΠ°ΠΊΠ΅ ΠΌΠΎΡΠ΅Π²ΠΎΠ³ΠΎ ΠΏΡΠ·ΡΡΡ ΠΈ Π΄ΡΡΠ³ΠΈΡ
), ΡΠ°ΠΊ ΠΈ Ρ ΠΏΠ°Π»Π»ΠΈΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅Π»ΡΡ (Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅, ΠΏΡΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΏΠ»Π΅Π²ΡΠΈΡΠ°Ρ
, ΠΎΠΏΡΡ
ΠΎΠ»ΡΡ
ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎ-ΠΊΠΈΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° ΠΈ Π΄ΡΡΠ³ΠΈΡ
). Π€ΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅ΡΠ°ΠΏΠΈΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ Π΄ΠΎΡΡΠΈΡΡ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ², Π½Π΅Π΄ΠΎΡΡΡΠΏΠ½ΡΡ
ΠΏΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ Π΄ΡΡΠ³ΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΏΡΠΎΡΠΈΠ²ΠΎΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ. Π’Π°ΠΊ, ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΈΠ·Π±Π΅ΠΆΠ°ΡΡ ΠΏΠΎΡΠ²Π»Π΅Π½ΠΈΡ Π³ΡΡΠ±ΡΡ
ΡΡΠ±ΡΠΎΠ² (ΡΡΠΎ ΠΎΡΠ΅Π½Ρ Π²Π°ΠΆΠ½ΠΎ, Π½Π°ΠΏΡΠΈΠΌΠ΅Ρ, Π² Π³ΠΈΠ½Π΅ΠΊΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΏΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ ΡΠ°ΠΊΠΎΠΌ ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ ΠΈ Π²ΡΠ»ΡΠ²Ρ ΡΠ΅ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ°), Π΄ΠΎΡΡΠΈΡΡ Ρ
ΠΎΡΠΎΡΠ΅Π³ΠΎ ΠΊΠΎΡΠΌΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΡΠ΅ΠΊΡΠ° ΠΏΡΠΈ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π½Π° ΠΊΠΎΠΆΠ΅, ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½ΠΎ ΡΡΠ°Π²ΠΌΠΈΡΠΎΠ²Π°ΡΡ ΠΎΠΊΡΡΠΆΠ°ΡΡΠΈΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»Ρ Π·Π΄ΠΎΡΠΎΠ²ΡΠ΅ ΡΠΊΠ°Π½ΠΈ. Π€ΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅ΡΠ°ΠΏΠΈΡ ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠΈΠΌΠ΅Π½ΡΠ΅ΡΡΡ ΠΈ Π² Π΄ΡΡΠ³ΠΈΡ
ΠΎΡΡΠ°ΡΠ»ΡΡ
ΠΌΠ΅Π΄ΠΈΡΠΈΠ½Ρ, ΡΠ°ΠΊΠΈΡ
ΠΊΠ°ΠΊ ΠΎΡΠΎΡΠΈΠ½ΠΎΠ»Π°ΡΠΈΠ½Π³ΠΎΠ»ΠΎΠ³ΠΈΡ, Π΄Π΅ΡΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡ, ΠΎΡΡΠ°Π»ΡΠΌΠΎΠ»ΠΎΠ³ΠΈΡ, ΠΎΡΡΠΎΠΏΠ΅Π΄ΠΈΡ, Π² Π»Π΅ΡΠ΅Π½ΠΈΠΈ ΠΏΠ°ΠΏΠΈΠ»Π»ΠΎΠΌΠ°Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ Π² Π»Π΅ΡΠ΅Π½ΠΈΠΈ Π³Π½ΠΎΠΉΠ½ΡΡ
ΡΠ°Π½ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π°Π½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ
Antiproliferative activity of PEP005, a novel ingenol angelate that modulates PKC functions, alone and in combination with cytotoxic agents in human colon cancer cells
PEP005 is a novel ingenol angelate that modulates protein kinases C (PKC) functions by activating PKCΞ΄ and inhibiting PKCΞ±. This study assessed the antiproliferative effects of PEP005 alone and in combination with several other anticancer agents in a panel of 10 human cancer cell lines characterised for expression of several PKC isoforms. PEP005 displayed antiproliferative effects at clinically relevant concentrations with a unique cytotoxicity profile that differs from that of most other investigated cytotoxic agents, including staurosporine. In a subset of colon cancer cells, the IC50 of PEP005 ranged from 0.01β140βΞΌM. The antiproliferative effects of PEP005 were shown to be concentration- and time-dependent. In Colo205 cells, apoptosis induction was observed at concentrations ranging from 0.03 to 3βΞΌM. Exposure to PEP005 also induced accumulation of cells in the G1 phase of the cell cycle. In addition, PEP005 increased the phosphorylation of PKCΞ΄ and p38. In Colo205 cells, combinations of PEP005 with several cytotoxic agents including oxaliplatin, SN38, 5FU, gemcitabine, doxorubicin, vinorelbine, and docetaxel yielded sequence-dependent antiproliferative effects. Cell cycle blockage induced by PEP005 in late G1 lasted for up to 24βh and therefore a 24βh lag-time between PEP005 and subsequent exposure to cytotoxics was required to optimise PEP005 combinations with several anticancer agents. These data support further evaluation of PEP005 as an anticancer agent and may help to optimise clinical trials with PEP005-based combinations in patients with solid tumours
Π€ΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅ΡΠ°ΠΏΠΈΡ Π±ΠΎΠ»ΡΠ½ΡΡ Ρ Π²Π½ΡΡΡΠΈΠΊΠΎΠΆΠ½ΡΠΌΠΈ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·Π°ΠΌΠΈ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ
Results of treating 114 skin metastases of melanoma in 21 patients are represented. Melanoma progression with cutaneous metastases after previous treatment was diagnosed in all patients. The method of prolonged photodynamic therapy developed by authors with fotosens administrated intravenously at dose of 0.4 mg/kg of body weight was used in the study. First session of tumor irradiation was performed 2 h after injection of photosensitizer. Patients underwent superfi cial or contact irradiation depending on topography and type of growth of metastatic foci. Course of treatment accounted for 7β10 sessions of photodynamic therapy with 24 h interval, light dose delivered to tumor for one session β 350 J/cm2. Signifi cant clinical effect (complete and partial tumor regression) was obtained in 89% of cases for developed method.Β ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ Π»Π΅ΡΠ΅Π½ΠΈΡ 114 ΠΌΠ΅ΡΠ°ΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠ°Π³ΠΎΠ² ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ Π² ΠΊΠΎΠΆΡ Ρ 21 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ°. Π£ Π²ΡΠ΅Ρ
Π±ΠΎΠ»ΡΠ½ΡΡ
Π±ΡΠ»ΠΎ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΠΏΡΠΎΠ³ΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ Ρ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π² ΠΊΠΎΠΆΡ ΠΏΠΎΡΠ»Π΅ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²ΡΡΡΠ΅Π³ΠΎ ΠΏΡΠΎΡΠΈΠ²ΠΎΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π° ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½Π°Ρ Π°Π²ΡΠΎΡΠ°ΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΠΏΡΠΎΠ»ΠΎΠ½Π³ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Ρ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠΌ ΡΠΎΡΠΎΡΠ΅Π½Ρ, ΠΊΠΎΡΠΎΡΡΠΉ Π²Π²ΠΎΠ΄ΠΈΠ»ΠΈ Π²Π½ΡΡΡΠΈΠ²Π΅Π½Π½ΠΎ Π² Π΄ΠΎΠ·Π΅ 0,4 ΠΌΠ³/ΠΊΠ³ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π° Π±ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ. ΠΠ΅ΡΠ²ΡΠΉ ΡΠ΅Π°Π½Ρ ΠΎΠ±Π»ΡΡΠ΅Π½ΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΎΡΠ°Π³ΠΎΠ² ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΠ΅ΡΠ΅Π· 2 Ρ ΠΏΠΎΡΠ»Π΅ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ ΡΠΎΡΠΎΡΠ΅Π½ΡΠΈΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΎΡΠ°. ΠΠ°ΡΠΈΠ΅Π½ΡΠ°ΠΌ Π²ΡΠΏΠΎΠ»Π½ΡΠ»ΠΈ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΠΎΠ΅ ΠΈΠ»ΠΈ ΠΊΠΎΠ½ΡΠ°ΠΊΡΠ½ΠΎΠ΅ ΠΎΠ±Π»ΡΡΠ΅Π½ΠΈΠ΅ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΡΠΎΠΏΠΎΠ³ΡΠ°ΡΠΈΠΈ ΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ° ΡΠΎΡΡΠ° ΠΌΠ΅ΡΠ°ΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ·Π»Π°. ΠΡΡΡ Π»Π΅ΡΠ΅Π½ΠΈΡ β 7β10 ΡΠ΅Π°Π½ΡΠΎΠ² ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Ρ ΠΈΠ½ΡΠ΅ΡΠ²Π°Π»ΠΎΠΌ Π² 24 Ρ, ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΡ ΡΠ½Π΅ΡΠ³ΠΈΠΈ Π»Π°Π·Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΡ, ΠΏΠΎΠ΄Π²ΠΎΠ΄ΠΈΠΌΠΎΠΉ ΠΊ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π·Π° ΠΎΠ΄ΠΈΠ½ ΡΠ΅Π°Π½Ρ β 350 ΠΠΆ/ΡΠΌ2. ΠΡΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΠΎΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½ ΠΎΠ±ΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΉ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΡΠ΅ΠΊΡ (ΠΏΠΎΠ»Π½Π°Ρ ΠΈ ΡΠ°ΡΡΠΈΡΠ½Π°Ρ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ) Π² 89% Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠΉ.
Clinical and psychological features of adolescents with the gastrointestinal diseases
The article examines the role of emotional-behavioral and cognitive-regulatory features of adolescents with diagnoses of gastroduodenitis, gastritis, dyskinesia of the bile ducts, dysfunction of the biliary tract, functional organ disorders digestion, etc. at the chronification of gastrointestinal diseases by the type of psychosomatic disorders. A comparison with a group of healthy teenagers is given.Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Π° ΡΠΎΠ»Ρ ΡΠΌΠΎΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎ-ΠΏΠΎΠ²Π΅Π΄Π΅Π½ΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΠΊΠΎΠ³Π½ΠΈΡΠΈΠ²Π½ΠΎ-ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ
ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΠΏΠΎΠ΄ΡΠΎΡΡΠΊΠΎΠ² Ρ Π΄ΠΈΠ°Π³Π½ΠΎΠ·Π°ΠΌΠΈ Π³Π°ΡΡΡΠΎΠ΄ΡΠΎΠ΄Π΅Π½ΠΈΡ, Π³Π°ΡΡΡΠΈΡ, Π΄ΠΈΡΠΊΠΈΠ½Π΅Π·ΠΈΡ ΠΆΠ΅Π»ΡΠ΅Π²ΡΠ²ΠΎΠ΄ΡΡΠΈΡ
ΠΏΡΡΠ΅ΠΉ, Π΄ΠΈΡΡΡΠ½ΠΊΡΠΈΡ Π±ΠΈΠ»ΠΈΠ°ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ°, ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΠΎΡΠ³Π°Π½ΠΎΠ² ΠΏΠΈΡΠ΅Π²Π°ΡΠ΅Π½ΠΈΡ ΠΈ Π΄Ρ. Π² Ρ
ΡΠΎΠ½ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎ-ΠΊΠΈΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° ΠΏΠΎ ΡΠΈΠΏΡ ΠΏΡΠΈΡ
ΠΎΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΡΡΡΡΠΎΠΉΡΡΠ². ΠΡΠΈΠ²Π΅Π΄Π΅Π½ΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ Ρ Π³ΡΡΠΏΠΏΠΎΠΉ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
ΠΏΠΎΠ΄ΡΠΎΡΡΠΊΠΎΠ²
Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ III ΡΠ°Π·Ρ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΡΠ°Π΄Π°Ρ Π»ΠΎΡΠΈΠ½ Π΄Π»Ρ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΏΡΠ΅Π΄ΡΠ°ΠΊΠ° ΠΈ Π½Π°ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΊΠ° ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ
The results of clinical study for efficacy of photodynamic therapy (PDT) with radachlorine in patients with pre-cancer and cancer of cervix are represented. The study enrolled 30 patients including 4 patients with cervical erosion, 5 patients with cervical intraepithelial neoplasia II, 13 patients with cervical intraepithelial neoplasia III, 4 patients with carcinoma in situ and 4 patients with cervical cancer stage Ia. Radachlorine was administrated as single 30 minute intravenous injection at dose of 1,0 mg/kg of body weight 3 h before irradiation (wavelength of 662 nm, light dose of 300β350 J/cm2). The results of treatment in 26 (86,7%) patients was assessed as complete tumor regression and in 4 (13,3%) patients β as partial regression. In cervical erosion, intraepithelial neoplasia II and carcinoma in situ groups total regression was in all cases. In the cervical intraepithelial neoplasia III group total regression after first course of PDT was achieved in 77% of patients, in cervical cancer stage Ia group β in 75% of patients. From 3 to 6 months after first course of treatment all patients with partial tumor regression underwent the second course of PDT with complete regression. There were no side-effects due to radachorine or PDT in the course of treatment and during follow-up. Thus, PDT with Russian photosensitizer radachlorine showed high efficiency for treatment of pre-cancer and cancer of cervix.Β ΠΡΠΈΠ²Π΅Π΄Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ (Π€ΠΠ’) Ρ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠΌ ΡΠ°Π΄Π°Ρ
Π»ΠΎΡΠΈΠ½ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ Ρ ΠΏΡΠ΅Π΄ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠ΅ΠΉ ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΎ 30 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Ρ ΡΡΠΎΠ·ΠΈΠ΅ΠΉ ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ β 4 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΊΠΈ, Ρ Π΄ΠΈΡΠΏΠ»Π°Π·ΠΈΠ΅ΠΉ II ΡΡ. β 5 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ, Ρ Π΄ΠΈΡΠΏΠ»Π°Π·ΠΈΠ΅ΠΉ III ΡΡ. β 13 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ, Ρ carcinoma in situ β 4 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΊΠΈ ΠΈ Ρ Π΄ΠΈΠ°Π³Π½ΠΎΠ·ΠΎΠΌ ΡΠ°ΠΊ ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ Ia ΡΡ. β 4 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΊΠΈ. Π Π°Π΄Π°Ρ
Π»ΠΎΡΠΈΠ½ Π²Π²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΎΠ΄Π½ΠΎΠΊΡΠ°ΡΠ½ΠΎ Π²Π½ΡΡΡΠΈΠ²Π΅Π½Π½ΠΎ ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²ΠΎΠΌ 30-ΠΌΠΈΠ½ΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΡΠ·ΠΈΠΈ Π² Π΄ΠΎΠ·Π΅ 1,0 ΠΌΠ³/ΠΊΠ³ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π° Π·Π° 3 Ρ Π΄ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΎΠ±Π»ΡΡΠ΅Π½ΠΈΡ (Π΄Π»ΠΈΠ½Π° Π²ΠΎΠ»Π½Ρ 662 Π½ΠΌ, ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΡ ΡΠ½Π΅ΡΠ³ΠΈΠΈ 300β350 ΠΠΆ/ΡΠΌ2). Π Π΅Π·ΡΠ»ΡΡΠ°Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ Ρ 26 (86,7%) ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ ΠΎΡΠ΅Π½Π΅Π½, ΠΊΠ°ΠΊ ΠΏΠΎΠ»Π½Π°Ρ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ, Ρ 4 (13,3%) β ΠΊΠ°ΠΊ ΡΠ°ΡΡΠΈΡΠ½Π°Ρ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΡ. Π Π³ΡΡΠΏΠΏΠ°Ρ
Ρ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌ Π΄ΠΈΠ°Π³Π½ΠΎΠ·ΠΎΠΌ ΡΡΠΎΠ·ΠΈΡ ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ, Π΄ΠΈΡΠΏΠ»Π°Π·ΠΈΡ II ΡΡ. ΠΈ carcinoma in situ ΠΏΠΎΠ»Π½Π°Ρ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΡ ΠΎΡΠΌΠ΅ΡΠ΅Π½Π° Π²ΠΎ Π²ΡΠ΅Ρ
Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΡΡ
. Π Π³ΡΡΠΏΠΏΠ°Ρ
Ρ Π΄ΠΈΡΠΏΠ»Π°Π·ΠΈΠ΅ΠΉ III ΡΡ. ΠΏΠΎΠ»Π½Π°Ρ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΡ ΠΏΠΎΡΠ»Π΅ ΠΏΠ΅ΡΠ²ΠΎΠ³ΠΎ ΠΊΡΡΡΠ° Π€ΠΠ’ Π΄ΠΎΡΡΠΈΠ³Π½ΡΡΠ° Ρ 77% ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠΊ, Ρ Π΄ΠΈΠ°Π³Π½ΠΎΠ·ΠΎΠΌ ΡΠ°ΠΊ ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ Ia ΡΡ. β Ρ 75% Π±ΠΎΠ»ΡΠ½ΡΡ
. Π£ Π²ΡΠ΅Ρ
Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΡΠ°ΡΡΠΈΡΠ½ΠΎΠΉ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΠ΅ΠΉ Π±ΡΠ» ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ Π²ΡΠΎΡΠΎΠΉ ΠΊΡΡΡ Π€ΠΠ’ Π² ΡΡΠΎΠΊ ΠΎΡ 3 Π΄ΠΎ 6 ΠΌΠ΅Ρ ΠΏΠΎΡΠ»Π΅ ΠΏΠ΅ΡΠ²ΠΎΠ³ΠΎ ΠΊΡΡΡΠ°, ΠΏΠΎΡΠ»Π΅ ΡΠ΅Π³ΠΎ Π±ΡΠ»Π° Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½Π° ΠΏΠΎΠ»Π½Π°Ρ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΡ. Π ΠΏΡΠΎΡΠ΅ΡΡΠ΅ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΈ ΠΏΡΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅ΠΌ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠΈ Π½Π΅ Π±ΡΠ»ΠΎ Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΠΊΠ°ΠΊΠΈΡ
-Π»ΠΈΠ±ΠΎ Π½Π΅ΠΆΠ΅Π»Π°ΡΠ΅Π»ΡΠ½ΡΡ
ΡΠ΅Π°ΠΊΡΠΈΠΉ, ΡΠ²ΡΠ·Π°Π½Π½ΡΡ
Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ°Π΄Π°Ρ
Π»ΠΎΡΠΈΠ½Π° ΠΈΠ»ΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ΠΌ Π€ΠΠ’. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, Π€ΠΠ’ Ρ ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΌ ΡΠΎΡΠΎΡΠ΅Π½ΡΠΈΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΎΡΠΎΠΌ ΡΠ°Π΄Π°Ρ
Π»ΠΎΡΠΈΠ½ ΠΏΠΎΠΊΠ°Π·Π°Π»Π° Π²ΡΡΠΎΠΊΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π² Π»Π΅ΡΠ΅Π½ΠΈΠΈ ΠΏΡΠ΅Π΄ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ.
Π€Π»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½Π°Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ° ΠΏΡΠ΅Π΄ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ
The technique of fluorescence hysteroscopy with Alasens includes visual assessment of fluorescence of Alasens-induced protoporphyrin IX and local fluorescence spectroscopy. The technique allows to improve the efficacy of early diagnosis for endometrial pathology including early endometrial cancer, to assess definitely an extent of pre-tumor and tumor process. The sensitivity of fluorescence hysteroscopy accounts for 100%, the specificity β 98%.Β Π’Π΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΠΎΠΉ Π³ΠΈΡΡΠ΅ΡΠΎΡΠΊΠΎΠΏΠΈΠΈ Ρ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠΌ Π°Π»Π°ΡΠ΅Π½Ρ Π²ΠΊΠ»ΡΡΠ°Π΅Ρ Π²ΠΈΠ·ΡΠ°Π»ΡΠ½ΡΡ ΠΎΡΠ΅Π½ΠΊΡ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠΈΠΈ Π°Π»Π°ΡΠ΅Π½Ρ-ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠΎΠΏΠΎΡΡΠΈΡΠΈΠ½Π° IX ΠΈ Π»ΠΎΠΊΠ°Π»ΡΠ½ΡΡ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΡΡ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΡ. Π’Π΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΠΎΠ²ΡΡΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠ°Π½Π½Π΅ΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ ΡΠ°Π½Π½ΠΈΡ
ΡΠΎΡΠΌ ΡΠ°ΠΊΠ° ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ, Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ ΠΎΡΠ΅Π½ΠΈΡΡ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΡ ΠΏΡΠ΅Π΄ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠ³ΠΎ ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°. Π§ΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΠΎΠΉ Π³ΠΈΡΡΠ΅ΡΠΎΡΠΊΠΎΠΏΠΈΠΈ ΡΠΎΡΡΠ°Π²Π»ΡΠ΅Ρ 100%, ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ 98%.
Changes of photosynthesis and carbon metabolism in Typha angustifolia L grown in conditions of nitrate nitrogen overload
Nitrates may induce alterations in NO-signaling system and change photosynthesisin plants. Significant reduction of 14CO2 fixation was noted at concentration of 3.96mM NaNO3 in an aquatic macrophyte (Typha angustifolia L.). Assimilation of 14CO2 seven days after the introduction of nitrates did not differ between control and experimental samples. There were changes in distribution of 14C among products of 4CO2 fixation 4 h after NaNO3 addition, resulting in increased sugar radioactivity in experimental plants. It was suggested that the observed changes may have regulatory importance
Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene.
Abstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 Β± 0.6 and 19.7 Β± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 Β± 1.4 vs. 18.0 Β± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene
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