Influence of nodular and severe forms of diffuse adenomyosis on reproductive function: a review of reproductive outcomes of surgical interventions and IVF

This review includes an analysis of clinical studies evaluating the effect of adenomyosis on reproductive function, as well as a review of studies on the relationship between surgical treat-ment options for adenomyosis and reproductive outcomes. Different studies of women populations after in vitro fertilization and/or intracytoplasmic sperm injection (IVF/ICSI) and after surgical treatment of  deep adenomyosis have shown that adenomyosis in both cases has a negative effect on the reproductive outcome, although there are significant differences in these populations. In the literature, there are relatively few data on the degree of influence of the depth of the lesion on the realization of the reproductive function, however, a significant correlation was revealed between them. The positive effect of treatment with gonadotropin-releasing hormone agonists, followed by surgical treatment of adenomyosis, has also been described in several literary sources, but there have been no controlled studies on this topic. Many studies do not meet the validity criteria due to the lack of clear criteria for inclusion and exclusion from the study.The choice of the optimal treatment options for adenomyosis based on the actual data is difficult due to the lack of reliable evidence of the relationship between fertility and the degree of adenomyosis. However, it is known that adenomyosis can reduce the likelihood of successful implantation so  ramatically that surgical or other treatment options are reasonably recom-mended. At the same time, it is necessary to assess the possible positive effect of the treatment options available in the doctor’s arsenal. Thus, timely referral to specialized treatment of ade-nomyosis may be crucial for the successful implementation of reproductive function in patients with suspicion or with an established diagnosis of adenomyosis in combination with recurrent miscarriage, who refuse to use assisted reproductive technologies, medical institutions. This review will analyze the relationship between infertility and adenomyosis, as well as review studies of reproductive outcomes in different options for surgical treatment of adenomyosis in infertile patients

Biochemical changes of synovial fluid in patients with osteoporosis of knee joint of various etiology

The aim of this study was to perform comparative analysis of biochemical composition of synovial fluid in patients with osteoarthrosis of various etiology. Studies were conducted on samples of synovial fluid of patients which were divided into two groups. Thefirstgroup consisted of 16 patients with patellofemoral arthrosis of the 2nd stage, the second group included 54 patients with bilateral gonarthrosis. The samples of synovial fluid of 11 cadavers of suddenly dead people of both sexes (4 men and 7 women) of 23-79 years who didn't have articular pathology registered by an expert were considered as a norm. Conducted study testifies significant imbalance of prooxidant and antioxidant processes in the synovial fluid and development of oxidative stress the intensity of which depends on the severity of the pathological process but is regardless to its etiology

Alumosilicate ceramic proppants based on natural refractory raw materials

The sintering-strengthening effect of the additions of the highly ferrous bauxite (with Fe[2]O[3] content of 20-25 % in the calcined state) in the compositions with refractory clays was established. It was found that in the temperature range 1350-1500°C the additions of bauxite in amounts of 10-40% have a fluxing effect due to the iron oxide introduced with bauxite in compositions with clay. An increasing the bauxite additive in the amount of 50-70% ensures its strengthening effect by increasing the total content of the mullite of the prismatic habit in the firing products of composites with clay. Preliminary clay and bauxite calcination at 900 °С and an increase in the content of bauxite additive up to 50-70% in compositions with clay allow to produce aluminosilicate proppants with a bulk density of 1.62-1.65 g/сm{3} and compressive strength up to 52 MPa

Evaluation of the effectiveness of age macular degeneration treatment

We examined 176 patients with an established diagnosis of age-related macular degeneration 'dry* form in the early and intermediate stages. The average age - 71,2 + 2,6, the average duration of the disease from the time of diagnosis of the disease 1,9 ± 1,3 years. All patients were divided into two groups: primary - 90 people and a comparison group of 86 people. The study group consisted of patients who underwent neyroprotektional vasodilator therapy: 5 mg Retinalamin subconjunctival injection number 10 and intramuscular injections of nicotinic acid 2,0 № 10. In the comparison group performed neuroprotective and vasodilator therapy in combination simpatocorrection number 10. The result has been found most effective therapeutic strategy: simpatocorrection in combination with the following regimen: 5 mg subconjunctival injection Retinalamin number 10, and intramuscular injection of nicotinic acid, 2.0 N° 10.Нами обследовано 176 больных с установленным диагнозом возрастной макулярной дегенерации «сухая» форма в ранней и промежуточной стадиях. Средний возраст-71,2 + 2,6, средняя продолжительность заболевания от момента диагностики заболевания 1,9+1,3 года. Все пациенты были разделены на две группы: основную - 90 человек и группу сравнения 86 человек. В основную группу вошли пациенты, которым была проведена нейропротекторнаяи сосудорасширяющая терапия: Ретиналамин 5 мг субконъюнктивально №10 и внутримышечные инъекции никотиновой кислоты 2,0 №10. В группе сравнения проводилась нейропротекторная и сосудорасширяющая терапия в сочетании симпатокоррекцией №10. В результате была выявлена наиболее эффективная терапевтическая стратегия, а именно, сочетание симпатокоррекции со следующей схемой лечения: Ретиналамин 5 мг субконъюнктивально № 10, внутримышечные инъекции никотиновой кислоты 2.0 №10

Электрофоретическая подвижность эритроцитов у больных с тяжелыми формами интоксикации

Objective: to study the diagnostic and prognostic value of changes in red blood cell electrophoretic mobility (RBCEM) in patients with infectious and noninfectious forms of intoxication.Materials and methods. A cytoferometer (Opton) was used to determine RBCEM; venous blood samples taken on heparin were studied. Thirty patients with peritonitis (infectious intoxication) and 22 patients with a pancreatic tumor and mechanical jaundice (noninfectious intoxication) were examined. All the patients received standard therapy for this disease. Eight out of 30 patients died on days 3—10 postoperatively.Results. On postoperative days 1—2, the values of RBCEM were outside the lower normal range in 90% of the patients with peritonitis (p<0.01). At the same time the patients with a good outcome of the disease differed from those who subsequently died (p<0.05), but they did not differ in the severity of the disease (APACHE II scale) and the results of laboratory and clinical blood tests. On days 7—14 versus days 1—2 after surgery, the survivors, unlike the deceased, showed an increase in RBCEM (p<0.05) and a reduction in disease severity by the APACHE II scale (p<0.05). On admission, in 95% of patients with pancreatic tumor, the values of RBCEM were beyond the lower normal range (p<0.01). Seven-day disintoxicant therapy caused a rise in this index.Conclusion. Intoxication with infectious and noninfectious agents leads to lower RBCEM. In patients with general peritonitis, the values of this index and its changes are of diagnostic and prognostic value in evaluating the severity and outcome of the disease and the efficiency of performed therapeutic measures. Цель. Изучение диагностической и прогностической значимости изменений электрофоретической подвижности эритроцитов (ЭФПЭ) у больных с инфекционной и неинфекционной формами интоксикации организма.Материалы и методы. Для определения ЭФПЭ был использован цитоферометр фирмы «Opton», материалом для исследования служила венозная кровь, взятая на гепарине. Обследование проведено у 30 больных с перитонитом (инфекционная форма интоксикации) и у 22 больных опухолью поджелудочной железы с механической желтухой (неинфекционная форма интоксикации). Всем больным проводили стандартизированную терапию для данного заболевания. Из 30 больных перитонитом 8 умерли на 3—10 сутки после операции.Результаты. На 1—2-е сутки после операции у 90% больных с перитонитом величины ЭФПЭ находились за пределами нижней границы нормы (р<0,01). При этом больные с благоприятным исходом заболевания отличались от впоследствии умерших более высокими значениями этого показателя (р<0,05), но не различались по тяжести состояния (шкала АРАСНЕ II), результатам лабораторно-клинического анализа крови. На 7—14-е сутки после операции у выживших больных, в отличие от умерших, наблюдалось увеличение ЭФПЭ (р<0,05) и уменьшение тяжести их состояния по шкале АРАСНЕ II (р<0,05) по сравнению с 1—2 сутками послеоперационного периода. При поступлении в стационар у 95% больных с опухолью поджелудочной железы величины ЭФПЭ были меньше нижней границы нормы (р<0,01). Проведение дезинтоксикационной терапии в течение 7 суток сопровождалось увеличением значений этого показателя.Заключение. Интоксикация организма инфекционными и неинфекционными агентами приводит к снижению ЭФПЭ. У больных с разлитым перитонитом значения этого показателя и его динамика имеют диагностическую и прогностическую значимость для оценки тяжести и исхода заболевания, эффективности проводимых лечебных мероприятий.

Investigation of oncolytic potential of vaccine strains of yellow fever and tick-borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell lines

Introduction. Flaviviruses, possessing natural neurotropicity could be used in glioblastoma therapy using attenuated strains or as a delivery system for antitumor agents in an inactivated form. Objective. To investigate the sensitivity of glioblastoma and pancreatic carcinoma cell lines to vaccine strains of yellow fever and tick-borne encephalitis viruses. Materials and methods. Cell lines: glioblastoma GL-6, T98G, LN-229, pancreatic carcinoma MIA RaCa-2 and human pancreatic ductal carcinoma PANC-1. Viral strains: 17D yellow fever virus (YF), Sofjin tick-borne encephalitis virus (TBEV). Virus concentration were determined by plaque assay and quantitative PCR. Determination of cell sensitivity to viruses by MTT assay. Results. 17D YF was effective only against pancreatic carcinoma tumor cells MIA Paca-2 and had a limited effect against PANC-1. In glioblastoma cell lines (LN229, GL6, T98G), virus had no oncolytic effect and the viral RNA concentration fell in the culture medium. Sofjin TBEV showed CPE50 against MIA Paca-2 and a very limited cytotoxic effect against PANC-1. However, it had no oncolytic effect against glioblastoma cell lines (LN229, T98G and GL6), although virus reproduction continued in these cultures. For the GL6 glioblastoma cell line, the viral RNA concentration at the level with the infection dose was determined within 13 days, despite medium replacement, while in the case of the LN229 cell line, the virus concentration increased from 1 × 109 to 1 × 1010 copies/ml. Conclusion. Tumor behavior in organism is more complex and is determined by different microenvironmental factors and immune status. In the future, it is advisable to continue studying the antitumor oncolytic and immunomodulatory effects of viral strains 17D YF and Sofjin TBEV using in vivo models

КИНЕМАТИЧЕСКИЙ ПОРТРЕТ ПАЦИЕНТА КАК ОБЪЕКТИВНЫЙ ПОКАЗАТЕЛЬ СОСТОЯНИЯ ДВИГАТЕЛЬНОЙ ФУНКЦИИ В ПРОЦЕССЕ НЕЙРОРЕАБИЛИТАЦИИ С ИСПОЛЬЗОВАНИЕМ ЭКЗОСКЕЛЕТА РУКИ, УПРАВЛЯЕМОГО ИНТЕРФЕЙСОМ «МОЗГ – КОМПЬЮТЕР»

The results of biomechanical analysis of the motor function of the arm of poststroke patient in the process of neuroreha bilitation with exoskeleton of the hand controlled by brain – computer interface are presented in this paper. At the beginning and end of the course it was registered the kinematic portrait of the patient– isolated random movements for each of the seven degrees of freedom as the paretic and intact arms.Angular accelerations were taken as an assessment of muscle forces, the number of reverse movements was taken as an assessment of joint spasticity, and the kinematic content of the movement as a description of pathological synergy arising after stroke. These parameters give an objective numerical asses sment of motor function as well as of rehabilitation technology effectiveness.Приведены результаты биомеханического анализа двигательной функции (ДФ) руки постинсультного больного в процессе проведения курса нейрореабилитации с использованием экзоскелета кисти, управляемого интерфейсом «мозг–компьютер». В начале и по окончании курса регистрировался кинематический портрет пациента – произвольные изолированные движения по каждой из семи степеней свободы как паретичной, так и сохранной руки. Угловые ускорения принимались в качестве оценки силы мышц, число возвратных движений – в качестве оценки спастичности сустава, кинематический состав движения – в качестве описания патологической синергии, возникшей после инсульта. Эти параметры дают объективную численную оценку как состояния ДФ, так и эффективности реабилитационных процедур

The MYST-Containing Protein Chameau Is Required for Proper Sensory Organ Specification during Drosophila Thorax Morphogenesis

The adult thorax of Drosophila melanogaster is covered by a stereotyped pattern of mechanosensory bristles called macrochaetes. Here, we report that the MYST containing protein Chameau (Chm) contributes to the establishment of this pattern in the most dorsal part of the thorax. Chm mutant pupae present extra-dorsocentral (DC) and scutellar (SC) macrochaetes, but a normal number of the other macrochaetes. We provide evidences that chm restricts the singling out of sensory organ precursors from proneural clusters and genetically interacts with transcriptional regulators involved in the regulation of achaete and scute in the DC and SC proneural cluster. This function of chm likely relies on chromatin structure regulation since a protein with a mutation in the conserved catalytic site fails to rescue the formation of supernumerary DC and SC bristles in chm mutant flies. This is further supported by the finding that mutations in genes encoding chromatin modifiers and remodeling factors, including Polycomb group (PcG) and Trithorax group (TrxG) members, dominantly modulate the penetrance of chm extra bristle phenotype. These data support a critical role for chromatin structure modulation in the establishment of the stereotyped sensory bristle pattern in the fly thorax