Reduced glutathione as a persistence indicator of alien plants of the Amelancheir family

Доведено, що глутатіон відновлений – важливий показник стану та стійкості рослинності. Досліджені види ірги (Amelancheir Med.) за вмістом глутатіону відновленого визнані адаптованими до умов середовища. Встановлено, що зростання вмісту глутатіону відновленого викликане несприятливим впливом абіотичних факторів, зокрема температури. Деякі розбіжності вмісту глутатіону відновленого пояснюються видовими властивостями рослин. Доказано, что глутатион восстановленный является важным показателем состояния и устойчивости растений. Исследованные виды ирги (Amelancheir Med.) по содержанию глутатиона восстановленного признаны адаптированными к условиям среды. Установлено, что увеличение содержания глутатиона восстановленного вызвано негативным влиянием абиотических факторов, в частности температуры. Некоторые расхождения в содержании глутатиона восстановленного объясняются видовыми свойствами растений.It was proved that glutathione is an important indicator of the vegetation condition and persistence. According to the amount of glutathione the studied mespilus species are adapted to the environmental conditions. Increase of the glutathione amount is caused by some abiotic factors, e.g. temperature. Some differences of the glutathione content may be explained by the plants species patterns

Growth of nano dots on the grazing incidence mirror surface under FEL irradiation Analytic approach to modeling

Simple analytic equation is deduced to explain new physical phenomenon detected experimentally growth of nano dots 40 55 nm diameter, 8 13 nm height, 9.4 dots amp; 956;m2 surface density on the grazing incidence mirror surface under the three years irradiation by the free electron laser FLASH 5 45 nm wavelength, 3 degrees grazing incidence angle . The growth model is based on the assumption that the growth of nano dots is caused by polymerization of incoming hydrocarbon molecules under the action of incident photons directly or photoelectrons knocked out from a mirror surface. The key feature of our approach consists in that we take into account the radiation intensity variation nearby a mirror surface in an explicit form, because the polymerization probability is proportional to it. We demonstrate that the simple analytic approach allows to explain all phenomena observed in experiment and to predict new effects. In particular, we show that the nano dots growth depends crucially on the grazing angle of incoming beam and its intensity growth of nano dots is observed in the limited from above and below intervals of the grazing angle and the radiation intensity. Decrease in the grazing angle by 1 degree only from 3 to 2 degree may result in a strong suppression of nanodots growth and their total disappearing. Similarly, decrease in the radiation intensity by several times replacement of free electron laser by synchrotron results also in disappearing of nano dots growt

Effect of the surface roughness on X ray absorption by mirrors operating at extremely small grazing angles

This study theoretically analyzes an increase in X ray absorption by a grazing incidence mirror due to its surface roughness. We demonstrate that the increase in absorption can be several hundred times larger than predicted by the Nevot Croce formula. As a result, absorption enhances by several times compared to a perfectly smooth mirror despite the extremely small grazing angle of an incident X ray beam a fraction of the critical angle of the total external reflection and the high quality of the reflecting surface the roughness height was 0.5 nm in modeling . The main contribution to the absorption increase was dictated by the mid scale roughness waviness of the virgin substrate surface, whose quality thus defines an absorption enhancement. The approach was applied to the analysis of two real mirrors used in a synchrotron BESSY I and a European X ray free electron laser XFEL beamline. The modern surface finishing technology of elastic emission machining provides extremely low substrate waviness, guaranteeing the negligible effect of the surface roughness on the absorption increas

Effect of intraosseous introduction of selenium/arabinogalactan nanoglycoconjugate on the main indicators of primary metabolism in consolidation of bone fracture

The research was carried out on 9 rabbits (males) of Chinchilla breed with modeling of standard perforating fracture of femoral bone. We performed intraosseous introduction of nanocomposite elemental selenium and heteropolysaccharide arabinogalactan 50 mg Se/kg to individuals of experimental group (n = 3) and NaCl 0,9 % to individuals of control group (n = 6). It was established that local intraosseous introduction of the nanocomposite with perforated fracture model did not affect basal metabolic indicators (body temperature, oxygen consumption, carbon dioxide production), but boosted the metabolic processes in the area of the surgical wound from the 9th to 21st days. It proves the bioavailability of the drug and the possibility of creating a local depot of selenium using a nanocomposite

A hard x ray split and delay unit for the HED experiment at the European XFEL

For the High Energy Density HED experiment [1] at the European XFEL [2] an x ray split and delay unit SDU is built covering photon energies from 5 keV up to 20 keV [3]. This SDU will enable time resolved x ray pump x ray probe experiments [4,5] as well as sequential diffractive imaging [6] on a femtosecond to picosecond time scale. Further, direct measurements of the temporal coherence properties will be possible by making use of a linear autocorrelation [7,8]. The set up is based on geometric wavefront beam splitting, which has successfully been implemented at an autocorrelator at FLASH [9]. The x ray FEL pulses are split by a sharp edge of a silicon mirror coated with multilayers. Both partial beams will then pass variable delay lines. For different photon energies the angle of incidence onto the multilayer mirrors will be adjusted in order to match the Bragg condition. For a photon energy of h amp; 957; 20 keV a grazing angle of amp; 952; 0.57 has to be set, which results in a footprint of the beam 6 amp; 963; on the mirror of l 98 mm. At this photon energy the reflectance of a Mo B4C multi layer coating with a multilayer period of d 3.2 nm and N 200 layers amounts to R 0.92. In order to enhance the maximum transmission for photon energies of h amp; 957; 8 keV and below, a Ni B4C multilayer coating can be applied beside the Mo B4C coating for this spectral region. Because of the different incidence angles, the path lengths of the beams will differ as a function of wavelength. Hence, maximum delays between 2.5 ps at h amp; 957; 20 keV and up to 23 ps at h amp; 957; 5 keV will be possibl

Distribution of microbial population as a cause and consequence of the anti-bacterial therapy failure of ventilator-associated pneumonia

Goal of the study: to study the specific distribution of microbial population in the lungs in the patients being on artificial pulmonary ventilation for a continuous period of time basing on the results of bacteriological culture of the specimens collected from various parts of the lungs and tracheobronchial system through intravital broncoscopy or post mortem.Materials and methods. Pilot study including intravital and post mortem identification of microbial population in various parts of the lungs and tracheobronchial system in 18 patients with signs of ventilator-associated pneumonia developed after cardiac-vascular and neurosurgical interventions. Intravital microbiological tests of the aspirate collected from all lobar bronchi of both lungs obtained through fiber-optic bronchoscopy were performed in 9 patients. Post mortem collection of pulmonary tissue for culture from all lobes of both lungs, lingulars of the left lung and relevant bronchi (totally 12 specimens from the lungs and bronchi) during 24 hours after death was performed in 9 patients.Results of the study. As per the data of microbiological tests the pulmonary infection was found in all patients from both groups. Intravital tests detected inter-lobar differences of microbial profile in 5 cases, and in 4 cases the differences were detected between the lungs. Post mortem microbiological tests detected differences between lobes and within lobes in 6 cases, of them in 5 cases there were also differences between the lungs. In all cases of nonuniform distribution detected post mortem, differences between lobes were accompanied by the differences within lobes (between tissue of the lungs and draining bronchus). Data analysis of intravital and post mortem microbiological diagnostics detected the similarity in the microbial population distribution in the tracheobronchial system in case of ventilator-associated pneumonia despite the different techniques of specimen collection. The diagnostic test capable to reflect regional distribution of the microbial population has been offered: aspirate culture from 5 lobar bronchi which could be used when managing patients with ventilator-associated pneumonia

COMPARISON OF VISCOSITY, GLASS TRANSITION TEMPERATURE AND DENSITY OF MELT xNa2O-(100-x) B2O3 FROM COMPOSITION

Viscosity and glass transition temperature are structural- sensitive properties and depend on alkali content in alkali-boron melts.Работа выполнена в рамках Госзадания ИМЕТ УрО РАН

A developmentally regulated translational control pathway establishes the meiotic chromosome segregation pattern

Production of haploid gametes from diploid progenitor cells is mediated by a specialized cell division, meiosis, where two divisions, meiosis I and II, follow a single S phase. Errors in progression from meiosis I to meiosis II lead to aneuploid and polyploid gametes, but the regulatory mechanisms controlling this transition are poorly understood. Here, we demonstrate that the conserved kinase Ime2 regulates the timing and order of the meiotic divisions by controlling translation. Ime2 coordinates translational activation of a cluster of genes at the meiosis I–meiosis II transition, including the critical determinant of the meiotic chromosome segregation pattern CLB3. We further show that Ime2 mediates translational control through the meiosis-specific RNA-binding protein Rim4. Rim4 inhibits translation of CLB3 during meiosis I by interacting with the 5′ untranslated region (UTR) of CLB3. At the onset of meiosis II, Ime2 kinase activity rises and triggers a decrease in Rim4 protein levels, thereby alleviating translational repression. Our results elucidate a novel developmentally regulated translational control pathway that establishes the meiotic chromosome segregation pattern.American Cancer Society (Post-doctoral Fellowship)Virginia and D.K. Ludwig Fund for Cancer Research (Post-doctoral Fellowship)National Institutes of Health (U.S.) (Grant GM62207

Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences

BACKGROUND: In cervical tumours the integration of human papilloma viruses (HPV) transcripts often results in the generation of transcripts that consist of hybrids of viral and cellular sequences. Mapping data using a variety of techniques has demonstrated that HPV integration occurred without obvious specificity into human genome. However, these techniques could not demonstrate whether integration resulted in the generation of transcripts encoding viral or viral-cellular sequences. The aim of this work was to map the integration sites of HPV DNA and to analyse the adjacent cellular sequences. METHODS: Amplification of the INTs was done by the APOT technique. The APOT products were sequenced according to standard protocols. The analysis of the sequences was performed using BLASTN program and public databases. To localise the INTs PCR-based screening of GeneBridge4-RH-panel was used. RESULTS: Twelve cellular sequences adjacent to integrated HPV16 (INT markers) expressed in squamous cell cervical carcinomas were isolated. For 11 INT markers homologous human genomic sequences were readily identified and 9 of these showed significant homologies to known genes/ESTs. Using the known locations of homologous cDNAs and the RH-mapping techniques, mapping studies showed that the INTs are distributed among different human chromosomes for each tumour sample and are located in regions with the high levels of expression. CONCLUSIONS: Integration of HPV genomes occurs into the different human chromosomes but into regions that contain highly transcribed genes. One interpretation of these studies is that integration of HPV occurs into decondensed regions, which are more accessible for integration of foreign DNA

Nanofocusing of hard X-ray free electron laser pulses using diamond based Fresnel zone plates

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