Characterization and expression analysis of Staphylococcus aureus pathogenicity island 3 - Implications for the evolution of staphylococcal pathogenicity islands

We describe the complete sequence of the 15.9-kb staphylococcal pathogenicity island 3 encoding staphylococcal enterotoxin serotypes B, K, and Q. The island, which meets the generally accepted definition of pathogenicity islands, contains 24 open reading frames potentially encoding proteins of more than 50 amino acids, including an apparently functional integrase. The element is bordered by two 17-bp direct repeats identical to those found flanking staphylococcal pathogenicity island 1. The island has extensive regions of homology to previously described pathogenicity islands, particularly staphylococcal pathogenicity islands 1 and bov. The expression of 22 of the 24 open reading frames contained on staphylococcal pathogenicity island 3 was detected either in vitro during growth in a laboratory medium or serum or in vivo in a rabbit model of toxic shock syndrome using DNA microarrays. The effect of oxygen tension on staphylococcal pathogenicity island 3 gene expression was also examined. By comparison with the known staphylococcal pathogenicity islands in the context of gene expression described here, we propose a model of pathogenicity island origin and evolution involving specialized transduction events and addition, deletion, or recombination of pathogenicity island "modules.

Salmonella Pathogenesis and Processing of Secreted Effectors by Caspase-3

The enteric pathogen Salmonella enterica serovar Typhimurium causes food poisoning resulting in gastroenteritis. The S. Typhimurium effector Salmonella invasion protein A (SipA) promotes gastroenteritis by functional motifs that trigger either mechanisms of inflammation or bacterial entry. During infection of intestinal epithelial cells, SipA was found to be responsible for the early activation of caspase-3, an enzyme that is required for SipA cleavage at a specific recognition motif that divided the protein into its two functional domains and activated SipA in a manner necessary for pathogenicity. Other caspase-3 cleavage sites identified in S. Typhimurium appeared to be restricted to secreted effector proteins, which indicates that this may be a general strategy used by this pathogen for processing of its secreted effectors

Ultraslow propagation of matched pulses by four-wave mixing in an atomic vapor

We have observed the ultraslow propagation of matched pulses in nondegenerate four-wave mixing in a hot atomic vapor. Probe pulses as short as 70 ns can be delayed by a tunable time of up to 40 ns with little broadening or distortion. During the propagation, a probe pulse is amplified and generates a conjugate pulse which is faster and separates from the probe pulse before getting locked to it at a fixed delay. The precise timing of this process allows us to determine the key coefficients of the susceptibility tensor. The presence of gain in this system makes this system very interesting in the context of all-optical information processing.Comment: 5 pages, 4 figure

Extinction and backscatter measurements of Antarctic PSC's, 1987: Implications for particle and vapor removal

The temperature dependence is examined of optical properties measured in the Antarctic during 1987 at the 70 mb level (near 18 km), a level chosen to correlate the results with in situ measurements made from the NASA-Ames ER-2 aircraft during the 1987 Airborne Antarctic Ozone Experiment (AAOE). The data set consists of extinction measurements by Sam 2 inside the Antarctic polar vortex from May to October 1987; and backscatter measurements by the UV-DIAL (Ultraviolet Differential Absorption Lidar) system aboard the Ames DC-8 aircraft during selected AAOE flights. Observed trends are compared with results from a revised version of Pole and McCormick's model to classify the PSC observations by Type (1 or 2) and infer the temporal behavior of the ambient aerosol and ambient vapor mixing ratios. The sample figures show monthly ensembles of the 70-mb Sam 2 extinction ratio (the ratio of aerosol or PSC extinction to molecule extinction) as a function of NMC temperature at the beginning (June) and (October) of the 1987 Antarctic winter. Both ensembles show two rather distinct clusters of points: one oriented in the near vertical direction which depicts the change with temperature of the ambient aerosol extinction ratio; and a second cluster oriented in the near horizontal direction whose position on the vertical scale marks a change in particle phase (i.e., PSC formation) and whose length (the extinction enhancement related to that of the ambient aerosol) is an indicator of PSC type

Polar ozone

The observation and interpretation of a large, unexpected ozone depletion over Antarctica has changed the international scientific view of stratospheric chemistry. The observations which show the veracity, seasonal nature, and vertical structure of the Antarctic ozone hole are presented. Evidence for Arctic and midlatitude ozone loss is also discussed. The chemical theory for Antarctic ozone depletion centers around the occurrence of polar stratospheric clouds (PSCs) in Antarctic winter and spring; the climatology and radiative properties of these clouds are presented. Lab studies of the physical properties of PSCs and the chemical processes that subsequently influence ozone depletion are discussed. Observations and interpretation of the chemical composition of the Antarctic stratosphere are described. It is shown that the observed, greatly enhanced abundances of chlorine monoxide in the lower stratosphere are sufficient to explain much if not all of the ozone decrease. The dynamic meteorology of both polar regions is given, interannual and interhemispheric variations in dynamical processes are outlined, and their likely roles in ozone loss are discussed

Optimizing end-labeled free-solution electrophoresis by increasing the hydrodynamic friction of the drag-tag

We study the electrophoretic separation of polyelectrolytes of varying lengths by means of end-labeled free-solution electrophoresis (ELFSE). A coarse-grained molecular dynamics simulation model, using full electrostatic interactions and a mesoscopic Lattice Boltzmann fluid to account for hydrodynamic interactions, is used to characterize the drag coefficients of different label types: linear and branched polymeric labels, as well as transiently bound micelles. It is specifically shown that the label's drag coefficient is determined by its hydrodynamic size, and that the drag per label monomer is largest for linear labels. However, the addition of side chains to a linear label offers the possibility to increase the hydrodynamic size, and therefore the label efficiency, without having to increase the linear length of the label, thereby simplifying synthesis. The third class of labels investigated, transiently bound micelles, seems very promising for the usage in ELFSE, as they provide a significant higher hydrodynamic drag than the other label types. The results are compared to theoretical predictions, and we investigate how the efficiency of the ELFSE method can be improved by using smartly designed drag-tags.Comment: 32 pages, 11 figures, submitted to Macromolecule

Is the sigma-1 receptor a potential pharmacological target for cardiac pathologies? A systematic review.

Sigma-1 receptors are ligand-regulated chaperone proteins, involved in several cellular mechanisms. The aim of this systematic review was to examine the effects that the sigma-1 receptor has on the cardiovascular system. The interaction targets and proposed mechanisms of action of sigma-1 receptors were explored, with the aim of determining if the sigma-1 receptor is a potential pharmacological target for cardiac pathologies. This systematic review was conducted according to the PRISMA guidelines and these were used to critically appraise eligible studies. Pubmed and Scopus were systematically searched for articles investigating sigma-1 receptors in the cardiovascular system. Papers identified by the search terms were then subject to analysis against pre-determined inclusion criteria. 23 manuscripts met the inclusion criteria and were included in this review. The experimental platforms, experimental techniques utilised and the results of the studies were summarised. The sigma-1 receptor is found to be implicated in cardioprotection, via various mechanisms including stimulating the Akt-eNOS pathway, and reduction of Ca2 + leakage into the cytosol via modulating certain calcium channels. Sigma-1 receptors are also found to modulate other cardiac ion channels including different subtypes of potassium and sodium channels and have been shown to modulate intracardiac neuron excitability. The sigma-1 receptor is a potential therapeutic target for treatment of cardiac pathologies, particularly cardiac hypertrophy. We therefore suggest investigating the cardioprotective mechanisms of sigma-1 receptor function, alongside proposed potential ligands that can stimulate these functions

SMART Vaccines 2.0 decision-support platform : A tool to facilitate and promote priority setting for sustainable vaccination in resource-limited settings

Funding Information: Supported by Gavi and the Bill and Melinda Gates Foundation, a number of international organisations have offered capacity-building support to establish NITAGs. While greater emphasis was initially placed on fulfilling process indicators for establishing NITAGs, more recent efforts have sought to advance functional capabilities associated with EIDM, most notably by Agence de Médecine Préventive (AMP), the International Vaccine Institute and The Sabin Vaccine Institute.13 14 These programmes have additionally leveraged technical assistance from WHO and its regional offices, PATH and the US Centers for Disease Control and Prevention.15 16 Funding Information: The UNITAG sought technical assistance from AMP’s Supporting Independent Vaccine Advisory Committees (SIVAC) Initiative,14and engaged in piloting the SMART Vaccines 2.0 platform supported by the Fogarty International Center at the US National Institutes of Health (NIH). A description of the NITAG process is given elsewhere.24 33 Funding Information: Funding This work was supported by the Fogarty International Center, National Institutes of Health, USA. Publisher Copyright: © 2020 Author(s). Published by BMJ.Peer reviewedPublisher PD

Informing Management of Henrys Lake, Idaho, using an Integrated Catch-at-Age Model

Henrys Lake, Idaho, supports a popular fishery for Yellowstone Cutthroat Trout Oncorhynchus clarkii bouvieri and Yellowstone Cutthroat Trout × Rainbow Trout O. mykiss hybrids. A majority of the adult population of fish in Henrys Lake are of hatchery origin that were stocked as fingerlings. The fishery is closed to angling during the late winter and spring months, but fisheries managers are considering opening the fishery year-round with catch-and-release- only regulations or with a two-fish bag limit during the extended season. However, there is concern that the proposed management actions may negatively affect the current fishery. Therefore, we developed an integrated catch-atage model to estimate population parameters for trout in Henrys Lake and used a simulation model to evaluate alternative management actions. Results of this study suggest that catch and release of both Yellowstone Cutthroat Trout and hybrids would increase and that abundance of trout in the spring (i.e., the start of the traditional season) would decrease under both proposed bag limits. Losses in abundance can be mitigated by stocking additional fish as long as no more than approximately 1,520,000 Yellowstone Cutthroat Trout are stocked annually. If catch-and-release-only regulations are implemented during the newly proposed season, total harvest is expected to decrease compared to the current fishery due to additional catch-and-release mortality. Ultimately, managers will need to prioritize harvest or catch-and-release opportunity, both of which provide additional utility to anglers, when choosing how to proceed with bag limit regulations

Towards a liquid Argon TPC without evacuation: filling of a 6 m^3 vessel with argon gas from air to ppm impurities concentration through flushing

In this paper we present a successful experimental test of filling a volume of 6 m$^3$ with argon gas, starting from normal ambient air and reducing the impurities content down to few parts per million (ppm) oxygen equivalent. This level of contamination was directly monitored measuring the slow component of the scintillation light of the Ar gas, which is sensitive to {\it all} sources of impurities affecting directly the argon scintillation.Comment: 9 pages, 6 figures, to appear in Proc. 1st International Workshop towards the Giant Liquid Argon Charge Imaging Experiment (GLA2010), Tsukuba, March 201