Considering Childhood Obesity through an Investigation of Maternal/Infant Factors and the Acceptability of a Text Message-Based Intervention.

Ph.D. Thesis. University of Hawaiʻi at Mānoa 2018

Zinc erythrocyte protoporphyrin as marker of malaria risk in pregnancy - a retrospective cross-sectional and longitudinal study.

BACKGROUND The effects of iron interventions and host iron status on infection risk have been a recurrent clinical concern, although there has been little research on this interaction in pregnant women. METHODS Cross-sectional and longitudinal analyses were undertaken to determine the association of whole blood zinc erythrocyte protoporphyrin (ZPP) with malaria parasitaemia in pregnant women attending antenatal and delivery care at Montfort and Chikwawa Hospitals, Shire Valley, Malawi. Prevalence of antenatal, delivery and placental malaria was assessed in relation to maternal ZPP levels. The main outcome measures were prevalence of peripheral and placental Plasmodium falciparum parasitaemia and odds ratios of malaria risk. RESULTS A total of 4,103 women were evaluated at first antenatal visit, of whom at delivery 1327 were screened for peripheral and 1285 for placental parasitaemia. Risk of malaria at delivery (peripheral or placental) was higher in primigravidae (p < 0.001), and lower (peripheral) with use of intermittent preventive anti-malarials during pregnancy (p < 0.001). HIV infection was associated with increased malaria parasitaemia (p < 0.02, peripheral or placental). Parasitaemia prevalence was lower in women with normal ZPP levels compared to those with raised concentrations at both first antenatal visit (all gravidae, p = 0.048, and at delivery (all gravidae, p < 0.001; primigravidae, p = 0.056). Between first antenatal visit and delivery women who transitioned from raised (at first antenatal visit) to normal ZPP values (at delivery) had lower peripheral parasitaemia prevalence at delivery compared to those who maintained normal ZPP values at both these visits (all gravidae: 0.70, 95%CI 0.4-1.1; primigravidae: 0.3, 0.1-0.8). In regression analysis this difference was lost with inclusion of HIV infection in the model. CONCLUSIONS Raised ZPP concentrations in pregnancy were positively associated with P. falciparum parasitaemia and were probably secondary to malaria inflammation, rather than indicating an increased malaria risk with iron deficiency. It was not possible from ZPP measurements alone to determine whether iron deficiency or repletion alters malaria susceptibility in pregnancy

Ionic Coulomb blockade and the determinants of selectivity in the NaChBac bacterial sodium channel

Mutation-induced transformations of conductivity and selectivity in NaChBac bacterial channels are studied experimentally and interpreted within the framework of ionic Coulomb blockade (ICB), while also taking account of resonant quantised dehydration (QD) and site protonation. Site-directed mutagenesis and whole-cell patch-clamp experiments are used to investigate how the fixed charge Qf at the selectivity filter (SF) affects both valence selectivity and same-charge selectivity. The new ICB/QD model predicts that increasing |Qf | should lead to a shift in selectivity sequences towards larger ion sizes, in agreement with the present experiments and with earlier work. Comparison of the model with experimental data leads to the introduction of an effective charge Qf∗ at the SF, which was found to differ between Aspartate and Glutamate charged rings, and also to depend on position within the SF. It is suggested that protonation of the residues within the restricted space of the SF is important in significantly reducing the effective charge of the EEEE ring. Values of Qf∗ derived from experiments on divalent blockade agree well with expectations based on the ICB/QD model and have led to the first demonstration of ICB oscillations in Ca2+ conduction as a function of the fixed charge. Preliminary studies of the dependence of Ca2+ conduction on pH are qualitatively consistent with the predictions of the model

Rest-frame properties of 32 gamma-ray bursts observed by the Fermi Gamma-Ray Burst Monitor

Aims: In this paper we study the main spectral and temporal properties of gamma-ray bursts (GRBs) observed by Fermi/GBM. We investigate these key properties of GRBs in the rest-frame of the progenitor and test for possible intra-parameter correlations to better understand the intrinsic nature of these events. Methods: Our sample comprises 32 GRBs with measured redshift that were observed by GBM until August 2010. 28 of them belong to the long-duration population and 4 events were classified as short/hard bursts. For all of these events we derive, where possible, the intrinsic peak energy in the $\nu F_{\nu}$ spectrum (\eprest), the duration in the rest-frame, defined as the time in which 90% of the burst fluence was observed (\tninetyrest) and the isotropic equivalent bolometric energy (\eiso). Results: The distribution of \eprest has mean and median values of 1.1 MeV and 750 keV, respectively. A log-normal fit to the sample of long bursts peaks at ~800 keV. No high-\ep population is found but the distribution is biased against low \ep values. We find the lowest possible \ep that GBM can recover to be ~ 15 keV. The \tninetyrest distribution of long GRBs peaks at ~10 s. The distribution of \eiso has mean and median values of $8.9\times 10^{52}$ erg and $8.2 \times 10^{52}$ erg, respectively. We confirm the tight correlation between \eprest and \eiso (Amati relation) and the one between \eprest and the 1-s peak luminosity ($L_p$) (Yonetoku relation). Additionally, we observe a parameter reconstruction effect, i.e. the low-energy power law index $\alpha$ gets softer when \ep is located at the lower end of the detector energy range. Moreover, we do not find any significant cosmic evolution of neither \eprest nor \tninetyrest.Comment: accepted by A&

Exploring the pore charge dependence of K+ and Cl- permeation across a graphene monolayer:a Molecular Dynamics study

Selective permeation through graphene nanopores is attracting increasing interest as an efficient and cost-effective technique for water desalination and purification. In this work, using umbrella sampling and molecular dynamics simulations with constant electric field, we analyze the influence of pore charge on potassium and chloride ion permeation. As pore charge is increased, the barrier of the potential of mean force (PMF) gradually decreases until it turns into a well split in two subminima. While in the case of K+ this pattern can be explained as an increasing electrostatic compensation of the desolvation cost, in the case of Cl&#x100000; the pattern can be attributed to the accumulation of a concentration polarization layer of potassium ions screening pore charge. Th analysis of potassium PMFs in terms of forces revealed a conflicting influence on permeation of van der Waals and electrostatic forces that both undergo an inversion of their direction as pore charge is increased. Even if the most important transition involves the interplay between the electrostatic forces exerted by graphene and water, the simulations also revealed an important role of the changing distribution of potassium and chloride ions. The influence of pore charge on the orientation of water molecules was also found to affect the van der Waals forces they exert on potassium

Statistical theory of mixed-valence selectivity in biological ion channels

In this article we apply our multi-component statistical and linear response theory to the process of mixed-valence ionic transport in a biological sodium ion channel. We analyse the free energy spectra, and statistical fluctuations (which are proportional to the conductivity) to investigate conditions and optimal parameters required for selectivity

The dynamics of quasiparticles in a toy model of the KcsA biological ion channel

We study the highly-concerted motion of ions in a narrow biological ion channel (KcsA) by considering the notion of a quasiparticle, with specific focus on the transition process. Namely, we show that the ion entering or exiting the channel is correlated with the position of the quasiparticle. This result is of importance in the rate theories of ion conduction through narrow channels and artificial nanopores

Physics of selective conduction and point mutation in biological ion channels

We introduce a statistical and linear response theory of selective conduction in biological ion channels with multiple binding sites and possible point mutation. We derive an effective grand canonical ensemble and generalised Einstein relations for the selectivity filter, assuming strongly coordinated ionic motion, and allowing for ionic Coulomb blockade. The theory agrees well with data from the KcsA K+ channel and a mutant. We show that the Eisenman relations for thermodynamic selectivity follow from the condition for fast conduction and find that maximum conduction requires the binding sites to be nearly identica

Prehistory probability distribution of ionic transitions through a graphene nanopore

We analyze selective ionic conduction through an artificial nanopore in a single graphene sheet, using molecular dynamics simulations and the prehistory probability distribution. We assess position-dependent changes in the number and orientation of water molecules in the first and second hydration shells of the ion as it crosses the nanopore. We reveal coupling between an ionic double layer near the sheet to the statistical properties of the hydration shells