High-temperature annealing of AlN films grown on 4H-SiC

The effect of high-temperature annealing (HTA) at 1700 °C on AlN films grown on 4H-SiC substrates by metalorganic vapor phase epitaxy has been studied. It is shown that the structural quality of the AlN layers improves significantly after HTA similar to what has been demonstrated for AlN grown on sapphire. Dislocation densities reduce by one order of magnitude resulting in 8 × 108 cm-2 for a-type and 1 × 108 cm-2 for c-type dislocations. The high-temperature treatment removes pits from the surface by dissolving nanotubes and dislocations in the material. XRD measurements prove that the residual strain in AlN/4H-SiC is further relaxed after annealing. AlN films grown at higher temperature resulting in a lower as-grown defect density show only a marginal reduction in dislocation density after annealing. Secondary ion mass spectrometry investigation of impurity concentrations reveals an increase of Si after HTA probably due to in-diffusion from the SiC substrate. However, C concentration reduces considerably with HTA that points to an efficient carbon removal process (i.e., CO formation). © 2020 Author(s)

In vivo and in vitro evidence that intrinsic upper- and lower-limb skeletal muscle function is unaffected by ageing and disuse in oldest-old humans

Aim: To parse out the impact of advanced ageing and disuse on skeletal muscle function, we utilized both in vivo and in vitro techniques to comprehensively assess upper- and lower-limb muscle contractile properties in 8 young (YG; 25 6 years) and 8 oldest-old mobile (OM; 87 5 years) and 8 immobile (OI; 88 4 years) women. Methods: In vivo, maximal voluntary contraction (MVC), electrically evoked resting twitch force (RT), and physiological cross-sectional area (PCSA) of the quadriceps and elbow flexors were assessed. Muscle biopsies of the vastus lateralis and biceps brachii facilitated the in vitro assessment of single fibre-specific tension (Po). Results: In vivo, compared to the young, both the OM and OI exhibited a more pronounced loss of MVC in the lower limb [OM (60%) and OI (75%)] than the upper limb (OM = 51%; OI = 47%). Taking into account the reduction in muscle PCSA (OM = 10%; OI = 18%), only evident in the lower limb, by calculating voluntary muscle-specific force, the lower limb of the OI (40%) was more compromised than the OM (13%). However, in vivo, RT in both upper and lower limbs (approx. 9.8 N m cm 2) and Po (approx. 123 mN mm 2), assessed in vitro, implies preserved intrinsic contractile function in all muscles of the oldest-old and were well correlated (r = 0.81). Conclusion: These findings suggest that in the oldest-old, neither advanced ageing nor disuse, per se, impacts intrinsic skeletal muscle function, as assessed in vitro. However, in vivo, muscle function is attenuated by age and exacerbated by disuse, implicating factors other than skeletal muscle, such as neuromuscular control, in this diminution of function. Keywords in vitro, in vivo, oldest-old, sarcopeni

Age-dependent variations in the expression of myosin isoforms and myogenic factors during the involution of the proximal sesamoidean ligament of sheep

In ungulates the stability of the fetlock joint is dependent on several muscles, which are exposed to high stress and strain. Among those muscles, the proximal sesamoidean ligament or PSL (also known as the suspensory ligament or Ruini's elasto-tendinous organ) is organized at birth in layers of muscle fibres alternated with abundant tendinous tissue that, during the postnatal development, becomes the predominant tissue. In this study we analysed the PSL of the sheep at the age of 1, 30 and 180\u202fdays and determined the expression of several genes which either (a) are markers of muscle fibre growth and maturation, or (b) play a role as signal molecules. We observed an accelerated maturation, as indicated by the transition of MyHC isoform expression towards the slow isoforms and a reduced regenerative potential indicated by the low Pax7 expression and the altered Wnt signalling. We also found a specific myogenic expression pattern of MyoD, Myf5 and Myogenin in the developing PSL and high mRNA levels of specific fibrogenic factors, as TGF-\u3b21, that, undoubtedly, stimulate the growth of connective tissue. Our observations confirmed, at molecular level, the peculiarity of the fast involution observed in PSL a muscle that undergoes a very specific active differentiation process during early development, which implies myofibres involution and their replacement with connective tissue

Sarcopenia in very old women: interaction of aging and disuse on muscle function decline

Sarcopenia is the age-associated loss of skeletal muscle mass and function which happens in upper (UL) as in lower limbs (LL) being still unclear the relation between muscles functions and daily activities (i.e. independently walking or not). We studied the changes linked to age and disuse in very old women. Contractile properties of whole muscles and isolated single fibres from UL (biceps brachii) and LL (vastus lateralis) were measured in 10 elderly women (90-100 y) divided in two subgroups on the basis of regular mobility: usually walking (E-W) II\u2013 usually moving by wheel chair (E-NW). Five young women (Y: 20-30 y) were used as controls. The following parameters were determined in single fibres: cross sectional area (CSA), isometric force (Fo), and MHC isoforms composition; in the whole muscles: lean mass and maximal isometric strength. Single fibre measurements showed: similar degree of atrophy in the EW and E-NW vs.Y; no changes in Fo; a significant age-related increase in slow fibres more pronounced in E-W. Whole muscles testing demonstrate: lower lean mass and strength in E groups vs Y. No changes in UL between aged groups (0.84\ub10.03 vs 0.86\ub10.04 kg/m2; 18\ub110, and 21\ub18Nm; E-NW vs E-W). Reduced lean mass and torque in the LL in the non walking (2.58\ub10.04 vs 3.20\ub10.04 kg/m2 41\ub119 vs 64\ub120Nm; E-NW vs E-W p<0,05). Our data demonstrate that age-related sarcopenia results in overall lower muscle mass while contractile function seems to be more affected by daily utilization