Epidemiology, clinical characteristics, and antimicrobial susceptibility profiles of human clinical isolates of Staphylococcus intermedius group

ABSTRACT The veterinary pathogens in the Staphylococcus intermedius group (SIG) are increasingly recognized as causes of human infection. Shared features between SIG and Staphylococcus aureus may result in the misidentification of SIG in human clinical cultures. This study examined the clinical and microbiological characteristics of isolates recovered at a tertiary-care academic medical center. From 2013 to 2015, 81 SIG isolates were recovered from 62 patients. Patients were commonly ≥50 years old, diabetic, and/or immunocompromised. Documentation of dog exposure in the electronic medical record was not common. Of the 81 SIG isolates, common sites of isolation included 37 (46%) isolates from wound cultures and 17 (21%) isolates from respiratory specimens. Although less common, 10 (12%) bloodstream infections were documented in 7 unique patients. The majority of SIG (65%) isolates were obtained from polymicrobial cultures. In comparison to S. aureus isolates from the same time period, significant differences were noted in proportion of SIG isolates that were susceptible to doxycycline (74% versus 97%, respectively; P &lt; 0.001), trimethoprim-sulfamethoxazole (65% versus 97%, respectively; P &lt; 0.001), and ciprofloxacin (78% versus 59%, respectively; P &lt; 0.01). Methicillin resistance (MR) was detected in 12 (15%) of 81 SIG isolates. All MR isolates detected by an oxacillin disk diffusion test would have been misclassified as methicillin susceptible using a cefoxitin disk diffusion test. Thus, SIG is recovered from human clinical specimens, and distinction of SIG from S. aureus is critical for the accurate characterization of MR status in these isolates. </jats:p

Model Dichotomous Key for Plastics Identification

The purpose of this study was to investigate selected plastics when treated by means of heat, fire, solvents, and specific gravity tests for the development of a model dichotomous key. The study included: (1) a review of literature that contained a brief historical overview of plastics, an examination of their uses, advantages, and chemical make up, and the selection of four tests to be conducted that would reveal characteristics of the plastics, (2) an explanation of the equipment, materials, and procedures for each of the tests, (3) an analysis of the data obtained from the tests, and (4) a recommendation on how to use the plastics dichotomous key. Methods Specified plastics were donated for testing purposes by several plastics manufacturing companies. The plastics were cut to size and used as samples in four tests. These tests included the effects of heat, the fire test, the effects of solvents, and specific gravity. The test data was analyzed and arranged into a basic dichotomous key format. Conclusions The conclusions for this study are as follows: (1) A form of media is needed to help individuals identify common plastics; (2) a propane torch is too powerful of a flame source to use in the fire test; (3) discrepancies in the colors of the flames between the writer\u27s findings and reports from other sources were caused by an incomplete observation of the total flame on the writer\u27s part in the fire test; (4) contaminated solvents may have caused difficulties in determining effects on some of the plastics; and (5) specific gravity results may have been affected by inaccurate balance scale and graduated cylinder readings. Recommendations The following recommendations have been suggested for this study: (1) A gentler flame source, such as the Bunsen burner flame, should be used in the fire test; (2) all parts of the flame should be noted and recorded in the fire test; (3) fresh chemicals should be used for each test conducted in determining the effects of solvents; (4) because of its tendency to curl and stick to the sides of the graduated cylinder, cellulose acetate should be tested in a form other than photographic film in the specific gravity test; (5) to determine the identity of the plastics samples, testing and comparison of characteristics should follow the same order as that of the dichotomous key; and (6) a pilot study should be conducted using the dichotomous key as a testing instrument to be used determine the key\u27s validity. A questionnaire would to provide supplementary information

Culture of urine specimens by use of chromID CPS Elite medium can expedite Escherichia coli identification and reduce hands-on time in the clinical laboratory

Urine is one of the most common specimen types submitted to the clinical microbiology laboratory; the use of chromogenic agar is one method by which the laboratory might expedite culture results and reduce hands-on time and materials required for urine culture analysis. The objective of our study was to compare chromID CPS Elite (bioMérieux), a chromogenic medium, to conventional primary culture medium for evaluation of urine specimens. Remnant urine specimens (n = 200) were inoculated into conventional media and into chromID CPS Elite agar (chromID). The time to identification and consumables used were documented for both methods. Clinically significant pathogen(s) were recovered from 51 cultures using conventional media, with Escherichia coli being the most frequently recovered organism (n = 22). The rate of exact uropathogen agreement between conventional and chromogenic media was 82%, while overall categorical agreement was 83.5% The time interval between plating and final organism identification was decreased with chromID agar versus conventional media for E. coli (mean of 24.4 h versus 27.1 h, P < 0.001). Using chromID, clinically significant cultures required less hands-on time per culture (mean of 1 min and 2 s [1:02 min]) compared to conventional media (mean of 1:31 min). In addition, fewer consumables (2.4 versus 3.3 sticks and swabs) and rapid biochemical tests (1.0 versus 1.9) were necessary using chromID versus conventional media. Notably, antimicrobial susceptibility testing demonstrated good overall agreement (97.4%) between the chromID and conventional media for all antibiotics tested. chromID CPS Elite is accurate for uropathogen identification, reduces consumable usage, and may expedite the identification of E. coli in clinical specimens

Nesting Common Eider (Somateria mollissima) Population Quintuples in Northwest Greenland

Common eider (Somateria mollissima) populations in Greenland severely declined throughout the 20th century. As a result, in 2001, harvest regulations were changed and the length of the hunting season was reduced. Recent data suggest that these changes have been successful, and population regrowth is occurring. In the Avanersuaq District, northwest Greenland, only one systematic survey quantifying the number of nesting eiders had previously been conducted, in 1997 and 1998. Although this district had historically been identified as having the largest number of breeding eiders in Greenland, the 1997–98 survey results showed a relatively small estimated population of 5000 pairs. However, it is not known to what extent changes in hunting regulations have affected nesting abundance in this area. Therefore, the Avanersuaq District was systematically resurveyed during the 2009 breeding season, approximately 11 years after the previous survey. These results showed that the population had increased to 5.4 times its 1997–98 size, with an annual compounded growth rate of 15.3%. On a single island, nearly 4500 active nests were observed. Five islands had more than 2600 nests each and comprised 75% of the total nests counted. Along with historical information to account for additional nesting habitat not surveyed, the observed population growth rate from this study suggests that the overall Avanersuaq common eider breeding population size ranges from 25 000 to 30 000 pairs, or roughly half of the total estimated West Greenland breeding population. Despite the significance of the Avanersuaq District as a breeding area for common eiders, we have only limited information about this population. The effects of recent extensions of the hunting season on this population are also unknown, and the only wintering location information is based on a few individuals banded in the 1920s and 1940s. Additional research on migratory movements is suggested before any further changes are made to hunting regulations.Au cours du XXe siècle, les populations d’eiders à duvet (Somateria mollissima) ont connu un déclin considérable au Groenland. C’est pourquoi en 2001, le règlement relatif à la chasse a été modifié et la saison de chasse a été raccourcie. Selon des données récentes, ces changements ont porté fruits en ce sens que la population s’est accrue. Dans le district d’Avanersuaq, dans le nord-ouest du Groenland, seulement un relevé systématique ayant pour but de quantifier le nombre d’eiders nicheurs a été fait, et c’était en 1997-1998. Bien qu’au fil des ans, ce district a compté le plus grand nombre d’eiders reproducteurs du Groenland, le relevé de 1997-1998 avait établi que la population était relativement petite, avec une estimation de 5 000 paires. On ne sait toutefois pas dans quelle mesure la modification du règlement sur la chasse a eu des effets sur l’abondance d’eiders nichant dans la région. Par conséquent, le district d’Avarsuaq a systématiquement fait l’objet d’un autre relevé pendant la saison de reproduction de 2009, soit environ 11 ans après le relevé d’origine. Les résultats ont permis de constater que la population s’était accrue dans une mesure de 5,4 fois par rapport à sa taille de 1997-1998, ce qui correspondait à un taux d’accroissement annuel composé de 15,3 %. Sur une seule île, près de 4 500 nids actifs ont été observés. Cinq îles comptaient plus de 2 600 nids par île, ce qui représentait 75 % du nombre total de nids répertoriés. Jumelé aux données historiques tenant compte de l’habitat de nidification supplémentaire et non relevé, le taux d’accroissement de la population observé à partir de cette étude suggère que la taille de la population globale d’eiders à duvet nicheurs d’Avanersuaq varie de 25 000 à 30 000 paires, ce qui correspond à environ la moitié du total estimé de la population nicheuse de l’ouest du Groenland. Malgré l’importance que revêt le district d’Avanersuaq comme aire de reproduction de l’eider à duvet, nous ne possédons que des renseignements restreints au sujet de cette population. Les effets qu’aura le prolongement récent de la saison de chasse sur cette population sont également inconnus, et la seule information concernant la localisation de l’aire d’hivernage dont nous disposons a trait à quelques individus qui avaient été bagués dans les années 1920 et 1940. Par conséquent, il est suggéré de pousser les recherches relatives aux mouvements migratoires plus loin avant d’apporter d’autres changements au règlement de chasse