QCGAT mixer compound exhaust system design and static big model test report

A mixer exhaust system was designed to meet the proposed performance and exhaust jet noise goals for the AiResearch QCGAT engine. Some 0.35 scale models of the various nozzles were fabricated and aerodynamically and acoustically tested. Preliminary optimization, engine cycle matching, model test data and analysis are presented. A final mixer exhaust system is selected for optimum performance for the overall flight regime

The Application of CRISPR Technology to High Content Screening in Primary Neurons

Axon growth is coordinated by multiple interacting proteins that remain incompletely characterized. High content screening (HCS), in which manipulation of candidate genes is combined with rapid image analysis of phenotypic effects, has emerged as a powerful technique to identify key regulators of axon outgrowth. Here we explore the utility of a genome editingapproach referred to as CRISPR (Clustered Regularly Interspersed Palindromic Repeats) for knockout screening in primary neurons. In the CRISPR approach a DNA-cleaving Cas enzyme is guided to genomic target sequences by user-created guide RNA (sgRNA), where it initiates a double-stranded break that ultimately results in frameshift mutation and loss of protein production. Using electroporation of plasmid DNA that co-expresses Cas9enzyme and sgRNA, we first verified the ability of CRISPR targeting to achieve protein-level knockdown in cultured postnatal cortical neurons. Targeted proteins included NeuN (RbFox3) and PTEN, a well-studied regulator of axon growth. Effective knockdown lagged at least four days behind transfection, but targeted proteins were eventually undetectable by immunohistochemistry in \u3e 80% of transfected cells. Consistent with this, anti-PTEN sgRNA produced no changes in neurite outgrowth when assessed three days post-transfection. When week-long cultures were replated, however, PTEN knockdown consistently increased neurite lengths. These CRISPR-mediated PTEN effects were achieved using multi-well transfection and automated phenotypic analysis, indicating the suitability of PTEN as a positive control for future CRISPR-based screening efforts. Combined, these data establish an example of CRISPR-mediated protein knockdown in primary cortical neurons and its compatibility with HCS workflows

Programming of cardiovascular disease across the life-course.

Cardiovascular disease (CVD) is the leading cause of morbidity and mortality, affecting both developed and developing countries. Whilst it is well recognized that our risk of CVD can be determined by the interaction between our genetics and lifestyle, this only partly explains the variability at the population level. Based on these well-known risk factors, for many years, intervention and primary prevention strategies have focused on modifying lifestyle factors in adulthood. However, research shows that our risk of CVD can be pre-determined by our early life environment and this area of research is known as the Developmental Origins of Health and Disease. The aim of this review is to evaluate our current understanding of mechanisms underlying the programming of CVD. This article is part of a special issue entitled CV Aging.H.L. Blackmore is funded by the British Heart Foundation. S.E. Ozanne is a member of the MRC Metabolic Diseases Unit and funded by MRC grantMC_UU_12012/4.This is the accepted manuscript of a paper published in the Journal of Molecular and Cellular Cardiology (Blackmore HL, Ozanne SE, Journal of Molecular and Cellular Cardiology 2014, doi:10.1016/j.yjmcc.2014.12.006)

Perturbed Three Vortex Dynamics

It is well known that the dynamics of three point vortices moving in an ideal fluid in the plane can be expressed in Hamiltonian form, where the resulting equations of motion are completely integrable in the sense of Liouville and Arnold. The focus of this investigation is on the persistence of regular behavior (especially periodic motion) associated to completely integrable systems for certain (admissible) kinds of Hamiltonian perturbations of the three vortex system in a plane. After a brief survey of the dynamics of the integrable planar three vortex system, it is shown that the admissible class of perturbed systems is broad enough to include three vortices in a half-plane, three coaxial slender vortex rings in three-space, and `restricted' four vortex dynamics in a plane. Included are two basic categories of results for admissible perturbations: (i) general theorems for the persistence of invariant tori and periodic orbits using Kolmogorov-Arnold-Moser and Poincare-Birkhoff type arguments; and (ii) more specific and quantitative conclusions of a classical perturbation theory nature guaranteeing the existence of periodic orbits of the perturbed system close to cycles of the unperturbed system, which occur in abundance near centers. In addition, several numerical simulations are provided to illustrate the validity of the theorems as well as indicating their limitations as manifested by transitions to chaotic dynamics.Comment: 26 pages, 9 figures, submitted to the Journal of Mathematical Physic

Motivations and barriers for Western Australian broad-acre farmers to adopt carbon farming

Available online 28 April 2017Carbon farming policies aim to contribute to climate change mitigation, but their success strongly depends on whether landholders actually adopt desired practices or participate in offered programs. The Australian Government’s Carbon Farming Initiative and Emissions Reduction Fund policies were designed to incentivise the adoption of carbon farming practices. Although these policies have been active since December 2011, farmer engagement has been limited, and net emissions reductions low as a result. We surveyed broad-acre farmers in the Western Australian wheatbelt to explore their drivers and barriers to adopting carbon farming practices and participating in carbon farming policy programs. Drivers of adoption included knowledge and perception of co-benefits (for yield, productivity, and the environment), knowing another adopter, and believing that changes to farm management are an appropriate method to reduce Australia’s greenhouse gas emissions. Barriers to adoption included lack of information, uncertainty and costs. The key barrier to participation was policy and political uncertainty. The determinants of adoption and participation that we identify in our study offer important insights into how to best ensure the success of Australia’s land sector-based climate change policies. We conclude that, to increase landholder engagement, the co-benefits and climate change benefits of carbon farming practices must be actively promoted, and additional information is needed about the costs associated with adoption. Information diffusion is best achieved if it actively leverages landholder social networks. Finally, our results indicate that landholder buy-in to carbon farming could be greatly enhanced by achieving more continuity in Australian climate change policies and politics.Marit E. Kragt, Nikki P. Dumbrell, Louise Blackmor

Datafication and the practice of intelligence production

Datafication of social life affects what society regards as knowledge. Jasanoff’s regimes of sight framework provides three ideal-type models of authorised knowing in environmental data practice. This paper applies Jasanoff's framework for analysing intelligence practice through an exploratory empirical study of crime and intelligence practitioners in a selection of police services in Australia, New Zealand, Canada and the United States. The paper argues that the ‘view from somewhere’ (VFS) captures the essence of existing police intelligence practices in the four countries but the ‘view from nowhere’ (VFN) is emerging as a possible future for police intelligence – an approach promoted by technology companies and supported mainly by police leaders and managers. The paper investigates the challenges and limits of a shift by police from VFS to VFN in the production of intelligence; the challenges are primarily political, which threaten the dominance of police contextual knowledge over ‘scientific’ knowledge. These political challenges also have symbolic and material implications. The paper concludes that, because of these challenges, a complete shift from VFS to VFN is not likely to happen. At best the two models might co-exist with the latter subordinate to the imperatives of the former, resulting in further tension between sworn officers and civilians, organisational inertia, as well as technologies that may be under-utilised or abandoned

A perspective on vibration-induced size segregation of granular materials

Segregation of particulate mixtures is a problem of great consequence in industries involved with the handling and processing of granular materials in which homogeneity is generally required. While there are several factors that may be responsible for segregation in bulk solids, it is well accepted that nonuniformity in particle size is a fundamental contributor. When the granular material is exposed to vibrations, the question of whether or not convection is an essential ingredient for size segregation is addressed by distinguishing between the situation where vibrations are not sufficiently energetic to promote a mean flow of the bulk solid, and those cases where a convective flow does occur. Based on experimental and simulation results in the literature, as well as dynamical systems analysis of a recent model of a binary granular mixture, it is proposed that "void-filling" beneath large particles is a universal mechanism promoting segregation, while convection essentially provides a means of mixing enhancement

Method and Apparatus for Powered Descent Guidance

A method and apparatus for landing a spacecraft having thrusters with non-convex constraints is described. The method first computes a solution to a minimum error landing problem for a convexified constraints, then applies that solution to a minimum fuel landing problem for convexified constraints. The result is a solution that is a minimum error and minimum fuel solution that is also a feasible solution to the analogous system with non-convex thruster constraints