Quantification of Dehydroepiandrosterone, 17β-Estradiol, Testosterone, and Their Sulfates in Mouse Tissues by LC-MS/MS.

We report a high-performance, liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) assay to quantify without derivatizaton dehyroepiandrosterone (DHEA), 17β-estradiol (E2), testosterone (T), and their sulfates in serum and tissues. This assay functions well with multiple adipose depots, a previously unattained analysis. To delipidate and facilitate recovery, tissues were homogenized in acetonitrile, and the homogenate was frozen. The supernatant was evaporated, resuspended in an aqueous acetate buffer, and extracted with hexane to separate free (unconjugated) from sulfated steroids. Sulfated steroids in the aqueous medium were then hydrolyzed with sulfatase and extracted with hexane. Each extract was analyzed separately. HPLC resolution combined with the sensitivity and specificity of MS/MS allowed quantification of DHEA, E2, and T with 10, 10, and 5 fmol lower limits of quantification and linear ranges to 1 pmol. Application of the method to mouse serum and tissues reveals ranges of DHEA, E2, and T and their sulfates, and tissue-specific differences in steroid profile, especially white versus brown adipose. In addition, marginal decreases of T in all tissues and considerable increases in DHEA in male iWAT and eWAT in response to a high-fat diet further strengthen the inference regarding the role of steroid metabolism in adipogenesis. This assay permits detailed studies of interactions between adiposity and sex steroids in serum and tissues, including adipose

In-situ electrochemical fabrication of natural contacts on single nanowires

We report a template-based in-situ electrochemical method for fabricating natural electric contacts on single nanowires using a pair of cross-patterned electrodes. Such electric contacts are highly stable upon thermal cycling between room temperature and milli-Kelvin temperatures. Direct imaging of the single-nanowire contacts using scanning electron microscopy is also demonstrated.Comment: 13 pages, 4 figure

Correlation between fracture surface morphology and toughness in Zr-based bulk metallic glasses

Fracture surfaces of Zr-based bulk metallic glasses of various compositions tested in the as-cast and annealed conditions were analyzed using scanning electron microscopy. The tougher samples have shown highly jagged patterns at the beginning stage of crack propagation, and the length and roughness of this jagged pattern correlate well with the measured fracture toughness values. These jagged patterns, the main source of energy dissipation in the sample, are attributed to the formation of shear bands inside the sample. This observation provides strong evidence of significant “plastic zone” screening at the crack tip

Viscoelastic Behavior of Solid 4^4He

Over the last five years several experimental groups have reported anomalies in the temperature dependence of the period and amplitude of a torsional oscillator containing solid $^4$He. We model these experiments by assuming that $^4$He is a viscoelastic solid--a solid with frequency dependent internal friction. We find that while our model can provide a quantitative account of the dissipation observed in the torsional oscillator experiments, it only accounts for about 10% of the observed period shift, leaving open the possibility that the remaining period shift is due to the onset of superfluidity in the sample.Comment: 4 pages, 3 figure

A Comprehensive Analysis of 5G Heterogeneous Cellular Systems operating over κ\kappa-μ\mu Shadowed Fading Channels

Emerging cellular technologies such as those proposed for use in 5G communications will accommodate a wide range of usage scenarios with diverse link requirements. This will include the necessity to operate over a versatile set of wireless channels ranging from indoor to outdoor, from line-of-sight (LOS) to non-LOS, and from circularly symmetric scattering to environments which promote the clustering of scattered multipath waves. Unfortunately, many of the conventional fading models adopted in the literature to develop network models lack the flexibility to account for such disparate signal propagation mechanisms. To bridge the gap between theory and practical channels, we consider $\kappa$-$\mu$ shadowed fading, which contains as special cases, the majority of the linear fading models proposed in the open literature, including Rayleigh, Rician, Nakagami-m, Nakagami-q, One-sided Gaussian, $\kappa$-$\mu$, $\eta$-$\mu$, and Rician shadowed to name but a few. In particular, we apply an orthogonal expansion to represent the $\kappa$-$\mu$ shadowed fading distribution as a simplified series expression. Then using the series expressions with stochastic geometry, we propose an analytic framework to evaluate the average of an arbitrary function of the SINR over $\kappa$-$\mu$ shadowed fading channels. Using the proposed method, we evaluate the spectral efficiency, moments of the SINR, bit error probability and outage probability of a $K$-tier HetNet with $K$ classes of BSs, differing in terms of the transmit power, BS density, shadowing characteristics and small-scale fading. Building upon these results, we provide important new insights into the network performance of these emerging wireless applications while considering a diverse range of fading conditions and link qualities

Criterion for transformation of transverse domain wall to vortex or antivortex wall in soft magnetic thin-film nanostripes

We report on the criterion for the dynamic transformation of the internal structure of moving domain walls (DWs) in soft magnetic thin-film nanostripes above the Walker threshold field, Hw. In order for the process of transformation from transverse wall (TW) to vortex wall (VW) or antivortex wall (AVW) occurs, the edge-soliton core of the TW-type DW should grow sufficiently to the full width at half maximum of the out-of-plane magnetizations of the core area of the stabilized vortex (or antivortex) by moving inward along the transverse (width) direction. Upon completion of the nucleation of the vortex (antivortex) core, the VW (AVW) is stabilized, and then its core accompanies the gyrotropic motion in a potential well (hill) of a given nanostripe. Field strengths exceeding the Hw, which is the onset field of DW velocity breakdown, are not sufficient but necessary conditions for dynamic DW transformation

Control of carbon nanotube morphology by change of applied bias field during growth

Carbon nanotube morphology has been engineered via simple control of applied voltage during dc plasma chemical vapor deposition growth. Below a critical applied voltage, a nanotube configuration of vertically aligned tubes with a constant diameter is obtained. Above the critical voltage, a nanocone-type configuration is obtained. The strongly field-dependent transition in morphology is attributed primarily to the plasma etching and decrease in the size of nanotube-nucleating catalyst particles. A two-step control of applied voltage allows a creation of dual-structured nanotube morphology consisting of a broad base nanocone (~200 nm dia.) with a small diameter nanotube (~7 nm) vertically emanating from the apex of the nanocone, which may be useful for atomic force microscopy

Regulation and Identity of Florigen: Flowering Locus T Moves Center Stage

The transition from vegetative to reproductive growth is controlled by day length in many plant species. Day length is perceived in leaves and induces a systemic signal, called florigen, that moves through the phloem to the shoot apex. At the shoot apical meristem (SAM), florigen causes changes in gene expression that reprogram the SAM to form flowers instead of leaves. Analysis of flowering of Arabidopsis thaliana placed the CONSTANS/FLOWERING LOCUS T (CO/FT) module at the core of a pathway that promotes flowering in response to changes in day length. We describe progress in defining the molecular mechanisms that activate this module in response to changing day length and the increasing evidence that FT protein is a major component of florigen. Finally, we discuss conservation of FT function in other species and how variation in its regulation could generate different flowering behaviors