A study of the word association technique as a measure of occupational information

Thesis (Ed.D.)--Boston Universit

Sub-grouping and sub-functionalization of the RIFIN multi-copy protein family

<p>Abstract</p> <p>Background</p> <p>Parasitic protozoans possess many multicopy gene families which have central roles in parasite survival and virulence. The number and variability of members of these gene families often make it difficult to predict possible functions of the encoded proteins. The families of extra-cellular proteins that are exposed to a host immune response have been driven via immune selection to become antigenically variant, and thereby avoid immune recognition while maintaining protein function to establish a chronic infection.</p> <p>Results</p> <p>We have combined phylogenetic and function shift analyses to study the evolution of the RIFIN proteins, which are antigenically variant and are encoded by the largest multicopy gene family in <it>Plasmodium falciparum</it>. We show that this family can be subdivided into two major groups that we named A- and B-RIFIN proteins. This suggested sub-grouping is supported by a recently published study that showed that, despite the presence of the <it>Plasmodium </it>export (PEXEL) motif in all RIFIN variants, proteins from each group have different cellular localizations during the intraerythrocytic life cycle of the parasite. In the present study we show that function shift analysis, a novel technique to predict functional divergence between sub-groups of a protein family, indicates that RIFINs have undergone neo- or sub-functionalization.</p> <p>Conclusion</p> <p>These results question the general trend of clustering large antigenically variant protein groups into homogenous families. Assigning functions to protein families requires their subdivision into meaningful groups such as we have shown for the RIFIN protein family. Using phylogenetic and function shift analysis methods, we identify new directions for the investigation of this broad and complex group of proteins.</p

Multiwalled carbon nanotube: Luttinger liquid or not?

We have measured IV-curves of multiwalled carbon nanotubes using end contacts. At low voltages, the tunneling conductance obeys non-Ohmic power law, which is predicted both by the Luttinger liquid and the environment-quantum-fluctuation theories. However, at higher voltages we observe a crossover to Ohm's law with a Coulomb-blockade offset, which agrees with the environment-quantum-fluctuation theory, but cannot be explained by the Luttinger-liquid theory. From the high-voltage tunneling conductance we determine the transmission line parameters of the nanotubes.Comment: RevTeX, 4 pages, 2 EPS-figures, submitted to Phys. Rev. Let

Safety of intravenous thrombolysis for acute ischemic stroke in patients receiving antiplatelet therapy at stroke onset

&lt;p&gt;&lt;b&gt;Background and Purpose:&lt;/b&gt; Antiplatelets (APs) may increase the risk of symptomatic intracerebral hemorrhage (ICH) following intravenous thrombolysis after ischemic stroke.&lt;/p&gt; &lt;p&gt;&lt;b&gt;Methods:&lt;/b&gt; We assessed the safety of thrombolysis under APs in 11 865 patients compliant with the European license criteria and recorded between 2002 and 2007 in the Safe Implementation of Treatments in Stroke (SITS) International Stroke Thrombolysis Register (SITS-ISTR). Outcome measures of univariable and multivariable analyses included symptomatic ICH (SICH) per SITS Monitoring Study (SITS-MOST [deterioration in National Institutes of Health Stroke Scale &gt;= 4 plus ICH type 2 within 24 hours]), per European Cooperative Acute Stroke Study II (ECASS II [deterioration in National Institutes of Health Stroke Scale &gt;= 4 plus any ICH]), functional outcome at 3 months and mortality.&lt;/p&gt; &lt;p&gt;&lt;b&gt;Results:&lt;/b&gt; A total of 3782 (31.9%) patients had received 1 or 2 AP drugs at baseline: 3016 (25.4%) acetylsalicylic acid (ASA), 243 (2.0%) clopidogrel, 175 (1.5%) ASA and dipyridamole, 151 (1.3%) ASA and clopidogrel, and 197 (1.7%) others. Patients receiving APs were 5 years older and had more risk factors than AP nave patients. Incidences of SICH per SITS-MOST (ECASS II respectively) were as follows: 1.1% (4.1%) AP naive, 2.5% (6.2%) any AP, 2.5% (5.9%) ASA, 1.7% (4.2%) clopidogrel, 2.3% (5.9%) ASA and dipyridamole, and 4.1% (13.4%) ASA and clopidogrel. In multivariable analyses, the combination of ASA and clopidogrel was associated with increased risk for SICH per ECASS II (odds ratio, 2.11; 95% CI, 1.29 to 3.45; P = 0.003). However, we found no significant increase in the risk for mortality or poor functional outcome, irrespective of the AP subgroup or SICH definition.&lt;/p&gt; &lt;p&gt;&lt;b&gt;Conclusion:&lt;/b&gt; The absolute excess of SICH of 1.4% (2.1%) in the pooled AP group is small compared with the benefit of thrombolysis seen in randomized trials. Although caution is warranted in patients receiving the combination of ASA and clopidogrel, AP treatment should not be considered a contraindication to thrombolysis.&lt;/p&gt

The spectroscopic evolution of the symbiotic-like recurrent nova V407 Cygni during its 2010 outburst. I. The shock and its evolution

On 2010 Mar 10, V407 Cyg was discovered in outburst, eventually reaching V< 8 and detected by Fermi. Using medium and high resolution ground-based optical spectra, visual and Swift UV photometry, and Swift X-ray spectrophotometry, we describe the behavior of the high-velocity profile evolution for this nova during its first three months. The peak of the X-ray emission occurred at about day 40 with a broad maximum and decline after day 50. The main changes in the optical spectrum began at around that time. The He II 4686A line first appeared between days 7 and 14 and initially displayed a broad, symmetric profile that is characteristic of all species before day 60. Low-excitation lines remained comparatively narrow, with v(rad,max) of order 200-400 km/s. They were systematically more symmetric than lines such as [Ca V], [Fe VII], [Fe X], and He II, all of which showed a sequence of profile changes going from symmetric to a blue wing similar to that of the low ionization species but with a red wing extended to as high as 600 km/s . The Na I D doublet developed a broad component with similar velocity width to the other low-ionization species. The O VI Raman features were not detected. We interpret these variations as aspherical expansion of the ejecta within the Mira wind. The blue side is from the shock penetrating into the wind while the red wing is from the low-density periphery. The maximum radial velocities obey power laws, v(rad,max) t^{-n} with n ~ 1/3 for red wing and ~0.8 for the blue. (truncated)Comment: Accepted for publication, A&A (submitted: 9 Oct 2010; accepted: 1 Dec 2010) in press; based on data obtained with Swift, Nordic Optical Telescope, Ondrejov Observatory. Corrected typo, Fermi?LAT detection was at energies above 100 MeV (with thanks to C. C. Cheung

High Mass Triple Systems: The Classical Cepheid Y Car

We have obtained an HST STIS ultraviolet high dispersion Echelle mode spectrum the binary companion of the double mode classical Cepheid Y Car. The velocity measured for the hot companion from this spectrum is very different from reasonable predictions for binary motion, implying that the companion is itself a short period binary. The measured velocity changed by 7 km/ s during the 4 days between two segments of the observation confirming this interpretation. We summarize "binary" Cepheids which are in fact members of triple system and find at least 44% are triples. The summary of information on Cepheids with orbits makes it likely that the fraction is under-estimated.Comment: accepted by A

Clinical selection strategies to identify ischemic stroke patients with large anterior vessel occlusion: results from SITS-ISTR (Safe Implementation of Thrombolysis in Stroke International Stroke Thrombolysis Registry)

Background and Purpose—The National Institutes of Health Stroke Scale (NIHSS) correlates with presence of large anterior vessel occlusion (LAVO). However, the application of the full NIHSS in the prehospital setting to select patients eligible for treatment with thrombectomy is limited. Therefore, we aimed to evaluate the prognostic value of simple clinical selection strategies. Methods—Data from the Safe Implementation of Thrombolysis in Stroke International Stroke Thrombolysis Registry (January 2012–May 2014) were analyzed retrospectively. Patients with complete breakdown of NIHSS scores and documented vessel status were included. We assessed the association of prehospital stroke scales and NIHSS symptom profiles with LAVO (internal carotid artery, carotid-terminus or M1-segment of the middle cerebral artery). Results—Among 3505 patients, 23.6% (n=827) had LAVO. Pathological finding on the NIHSS item best gaze was strongly associated with LAVO (adjusted odds ratio 4.5, 95% confidence interval 3.8–5.3). All 3 face–arm–speech–time test (FAST) items identified LAVO with high sensitivity. Addition of the item gaze to the original FAST score (G-FAST) or high scores on other simplified stroke scales increased specificity. The NIHSS symptom profiles representing total anterior syndromes showed a 10-fold increased likelihood for LAVO compared with a nonspecific clinical profile. If compared with an NIHSS threshold of ≥6, the prehospital stroke scales performed similarly or even better without losing sensitivity. Conclusions—Simple modification of the face–arm–speech–time score or evaluating the NIHSS symptom profile may help to stratify patients’ risk of LAVO and to identify individuals who deserve rapid transfer to comprehensive stroke centers. Prospective validation in the prehospital setting is required

Tunneling between two Luttinger liquids with long range interaction

The non linear charge transfer through a tunnel junction between two Luttinger systems is studied for repulsive, finite range interaction between electrons on the same, V_{11}, and on different,V_{12}, sides of the junction. Features of the Coulomb blockade effect are observed if V_{12}=0. We predict a novel interaction induced enhancement of the current if V_{12}>0. When V_{12}=V_{11}, the current is suppressed at small bias, but the ``charging energy'', obtained from the asymptotic behavior at high bias voltage, vanishes.Comment: 4 pages, RevTeX, to be published in Physical Review B (Brief Report

Strong dust processing in circumstellar discs around 6 RV Tauri stars. Are dusty RV Tauri stars all binaries?

We present extended Spectral Energy Distributions (SEDs) of seven classical RV Tauri stars, using newly obtained submillimetre continuum measurements and Geneva optical photometry supplemented with literature data. The broad-band SEDs show a large IR excess with a black-body slope at long wavelengths in six of the seven stars, R Sct being the noticeable exception. This long wavelength slope is best explained assuming the presence of a dust component of large grains in the circumstellar material. We show that the most likely distribution of the circumstellar dust around the six systems is that the dust resides in a disc. Moreover, very small outflow velocities are needed to explain the presence of dust near the sublimation temperature and we speculate that the discs are Keplerian. The structure and evolution of these compact discs are as yet not understood but a likely prerequisite for their formation is that the dusty RV Tauri stars are binaries.Comment: 10 pages, will be published in A&