The Quiet Crisis

Most Americans find it difficult to conceive a land ethic for tomorrow. The pastoral American of a century ago, whose conservation insights were undeveloped, has been succeeded by the asphalt American of the 1960’s, who is shortsighted in other ways. Our sense of stewardship is uncertain partly because too many of us lack roots in the soil and the respect for resources that goes with such roots. Too many of us have mistaken material ease and comfort for the good life. Our growing dependence on machines has tended to mechanize our response to the world around us and has blunted our appreciation of the higher values

Comparison of VO2 peak during treadmill and cycle ergometry in severely overweight youth

The purpose of this study was to compare peak cardiorespiratory parameters during treadmill and cycle ergometry in severely overweight youth. Twenty-one participants from the Committed to Kids Pediatric Weight Management program at the Louisiana State University Health Sciences Center volunteered. Participants completed peak treadmill and cycle ergometer tests on separate days. In order to examine reliability, six subjects completed a second treadmill test and seven subjects a second cycle test. Physical characteristics included the following: Age (yrs) 12.5 ± 2.8; Body weight (BW) (kg) 78.5 ± 27.0, Height (m) 1.56 ± 0.13; and % fat 42.8 ± 7.5. No statistical significant differences (p ? 0.05) were found between treadmill and cycle peak tests. Treadmill VO2 peak (l·min-1) averaged 1.57 ± 0.40 and cycle 1.46 ± 0.30 and VO2 peak relative to BW 21.5 ± 4.1 and 20.3 ± 5.5 for treadmill and cycle ergometry, respectively. Therefore treadmill values were 7.0% and 5.6% higher than cycle values. In normal weight or children and adolescents at risk for overweight, treadmill values typically average from 7 to 12% higher than cycle values. Reliability of VO2 peak as indicated by intraclass correlation coefficients ranged from 0.70 to 0.96 for a single or repeated tests. Intra individual variability averaged 0.5% for VO2 peak (l·min-1) during treadmill ergometry and 5.7% for cycle ergometry. Also, standard errors of measurement were low (40 to 90 ml min or 1.0 to 1.7 ml.kg-1. min-1) for the peak treadmill or cycle tests. In summary, our data suggest that both treadmill and cycle ergometry provide reliable methods for determining VO2 peak in overweight youth

Development and mapping of SNP assays in allotetraploid cotton

A narrow germplasm base and a complex allotetraploid genome have made the discovery of single nucleotide polymorphism (SNP) markers difficult in cotton (Gossypiumhirsutum). To generate sequence for SNP discovery, we conducted a genome reduction experiment (EcoRI, BafI double digest, followed by adapter ligation, biotin–streptavidin purification, and agarose gel separation) on two accessions of G. hirsutum and two accessions of G. barbadense. From the genome reduction experiment, a total of 2.04 million genomic sequence reads were assembled into contigs with an N50 of 508 bp and analyzed for SNPs. A previously generated assembly of expressed sequence tags (ESTs) provided an additional source for SNP discovery. Using highly conservative parameters (minimum coverage of 8× at each SNP and 20% minor allele frequency), a total of 11,834 and 1,679 non-genic SNPs were identified between accessions of G. hirsutum and G. barbadense in genome reduction assemblies, respectively. An additional 4,327 genic SNPs were also identified between accessions of G. hirsutum in the EST assembly. KBioscience KASPar assays were designed for a portion of the intra-specific G. hirsutum SNPs. From 704 non-genic and 348 genic markers developed, a total of 367 (267 non-genic, 100 genic) mapped in a segregating F2 population (Acala Maxxa × TX2094) using the Fluidigm EP1 system. A G. hirsutum genetic linkage map of 1,688 cM was constructed based entirely on these new SNP markers. Of the genic-based SNPs, we were able to identify within which genome (‘A’ or ‘D’) each SNP resided using diploid species sequence data. Genetic maps generated by these newly identified markers are being used to locate quantitative, economically important regions within the cotton genome