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    Genetic Variability of Lucerne Landraces from Central Italy Detected by RAPD Markers

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    With the aim to characterize six lucerne landraces (Medicago sativa L.), representing a sample of a collection from central Italy, sixty individuals per landrace were evaluated by screening for RAPD markers with three lucerne-specific primers. Twenty-one amplification products were scored as present or absent across all plants. The dendrogram from mean genetic similarity estimates displayed Casalina alone and the other landraces clustered into one distinct group, showing a single branch point with more than 73% of genetic similarity. The discriminant analysis grouped the landraces in a similar manner. The first function maximally separated the group Grosseto, Gubbio and C. Pieve from Latina and L’Aquila while the second function maximally separated Casalina from the rest of landraces. Overall 56% of individual plants were correctly reclassified into their own groups. Owing to their rather narrow geographic provenance, more primers are needed to increase precision in the estimate of the genetic variability

    Air Quality and Comfort Characterisation within an Electric Vehicle Cabin in Heating and Cooling Operations†

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    This work is aimed at the experimental characterisation of air quality and thermal profile within an electric vehicle cabin, measuring at the same time the HVAC system energy consumption. Pollutant concentrations in the vehicle cabin are measured by means of a low-cost system of sensors. The effects of the HVAC system configuration, such as fresh-air and recirculation mode, on cabin air quality, are discussed. It is shown that the PM concentrations observed in recirculation mode are lower than those in fresh-air mode, while VOC concentrations are generally higher in recirculation than in fresh-air mode. The energy consumption is compared in different configurations of the HVAC system. The novelty of this work is the combined measurement of important comfort parameters such as air temperature distribution and air quality within the vehicle, together with the real time energy consumption of the HVAC system. A wider concept of comfort is enabled, based on the use of low-cost sensors in the automotive field

    Lipoprotein glomerulopathy treated with LDL-apheresis (Heparin-induced Extracorporeal Lipoprotein Precipitation system): a case report.

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    INTRODUCTION: Lipoprotein glomerulopathy is a glomerulonephritis which was described for the first time by Saito in 1989 and is currently acknowledged as a separate nosological entity. It is histologically characterized by a marked dilatation of the glomerular capillaries and the presence of lipoprotein thrombi in the glomerular lumens. The dyslipidemic profile is similar to that of type III dyslipoproteinemia with Apolipoprotein E values that are often high; proteinuria and renal dysfunction are present. Proteinuria often does not respond to steroid and cytostatic treatments. The phenotypic expression of lipoprotein glomerulopathy is most probably correlated to a genetic alteration of the lipoprotein metabolism (mutation of the Apolipoprotein E coding gene). In literature, lipoprotein glomerulopathies have mainly been reported in Japanese and Chinese subjects, except for three cases in the Caucasian race, reported in France and the USA.CASE PRESENTATION: We describe the case of a 60-year-old female, Caucasian patient suffering from lipoprotein glomerulopathy, carrier of a new mutation on the Apolipoprotein E gene (Apolipoprotein E(MODENA)), and treated successfully with low density lipoprotein-apheresis with the Heparin induced extracorporeal lipoprotein precipitation system. After a first phase of therapeutic protocol with statins, the patient was admitted for nephrotic syndrome, renal failure and hypertension. Since conventional treatment alone was not able to control dyslipidemia, aphaeretic treatment with heparin-induced Extracorporeal Lipoprotein Precipitation - apheresis (HELP-apheresis) was started to maintain angiotensin converting enzyme inhibitor therapy for the treatment of hypertension. Treatment with HELP-apheresis led to a complete remission of the proteinuria in a very short time (four months), as well as control of hypercholesterolemia and renal function recovery.CONCLUSION: According to this case of lipoprotein glomerulopathy, we believe that renal damage expressed by proteinuria correlates to the levels of lipids and, furthermore, the treatment with HELP-apheresis, by lowering low-density lipoprotein cholesterol and triglycerides, may be considered as a therapeutic option in synergy with pharmacological treatment in the treatment of lipoprotein glomerulopathy

    "Medikamente sind Bomben" - zum Metapherngebrauch von Lungentransplantations-Patienten mit guter oder ungenĂĽgender Compliance

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    Fragestellung: Nach einer Organtransplantation finden komplexe psychologische Verarbeitungsprozesse statt. In der vorliegenden Studie wurde untersucht, welche Metaphern im Zusammenhang mit Transplantationserfahrungen verwendet werden, und ob Unterschiede zwischen Patienten mit guter bzw. ungenügender Compliance im Gebrauch dieser Metaphern bestehen. Methode: 14 lungentransplantierte Patienten wurden in einem halbstrukturierten Interview zu ihren Transplantationserfahrungen befragt. Ihre Compliance wurde von den behandelnden Ärzten eingeschätzt. Die Auswertung der Interviews erfolgte anhand einer Metaphernanalyse, welche Hinweise auf vor- und unbewusste Vorstellungen der Patienten liefert. Die Interraterreliabilität über die Metapherngruppen war Cohen’s Kappa K = 0.8. Ergebnisse: Die Patienten konzeptualisierten ihren Körper, aber auch ihr Selbst als ein “Gefäß”, dass sowohl materielle (z.B. die Lunge) wie immaterielle Objekte (z.B. Gedanken an den Spender, Affekte) enthält. Der wichtigste Unterschied zwischen den Compliance-Gruppen bestand darin, dass Patienten mit ungenügender Compliance eine grössere Distanz zur transplantierten Lunge erlebten. Auch konzeptualisierten sie ihren Körper bzw. ihr Selbst nicht als ein Gefäß, das die Lunge enthält. Diskussion: Die Ergebnisse zeigen, dass gute Compliance mit einer erfolgreichen Organintegration assoziiert ist, welche die Zugehörigkeit der Lunge in einem als Gefäß konzeptualisierten Körper bzw. Selbst umfasst. Patienten mit ungenügender Compliance nehmen die Lunge eher als Fremdkörper wahr. Diese Verarbeitungsprozesse sind teils bewusster, teils vor- und unbewusster Natur

    Setas urticantes en tarantulas de Uruguay: Âżdefensa activa o pasiva?

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    Most of the New World tarantulas feature specialized setae as a defense mechanism. Two mechanisms of defense have been proposed for the urticating setae (US): active defense against potential predators, and passive defense against other arthropods by incorporating the US to the molting mat and egg sacs. Uruguayan tarantulas present three different US morphological types named: types I, III and IV. It has been proposed that type I is used in passive defense whereas type III serves its purpose in active defense. There are drastic differences of biological characteristics between adult females and males. Females live most of their entire life inside their burrows, while males wander when they reach adulthood, looking for females during the reproductive season. Considering these differences, diverse defense strategies should be expected. To assess the possible role of US in active/ passive defense strategies we have counted the number of US in the abdomen of individuals of four species while making comparisons between sexes. Significant differences were found between males and females of all sampled species, with females showing a predominance of types I (except subtype Ic) or IV setae over other types or subtypes, suggesting these type of US takes part in passive defense.La mayoría de las tarántulas del nuevo mundo presentan setas especializadas como defensa. Dos mecanismos de defensa han sido propuestos para las setas urticantes (US): defensa activa contra potenciales depredadores y defensa pasiva contra otros artrópodos mediante la incorporación de US a las telas de mudas u ootecas. Las tarántulas uruguayas presentan tres tipos morfológicos de US llamados: I, III y IV. Se ha propuesto que el tipo I se utiliza principalmente en defensa pasiva mientras que el tipo III en defensa activa. Hembras y machos adultos presentan diferencias drásticas en su biología. Las hembras permanecen la mayor parte de su vida en sus cuevas mientras que los machos, una vez que se hacen adultos, salen y buscan activamente hembras durante la época reproductiva. Considerando estas diferencias se presumen diferentes estrategias defensivas entre los sexos. Para conocer el uso de los diferentes tipos de US en defensa activa o pasiva se estudiaron las dotaciones de US en individuos de cuatro especies de tarántulas de Uruguay, comparando machos y hembras. Se encontraron diferencias sexuales en todas las especies, las hembras muestran predominancia de US de los tipos I (excepto subtipo Ic) o IV sobre otros tipos y subtipos de US, lo que sugiere su participación en defensa pasiva
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