Vineyards characteristic by using GIS and refl ectance measurements on the Nagy-Eged hill in Hungary

Our investigation was carried out in vineyards on Nagy-Eged hill in Hungary. Grapevine growing plays an important role in EgerVine Regions. In the grapevine cultivation it becomes necessary to know the requirements of vineyards along the best geographical situationusing available cultivation and examination technology to achieve better and quality produce. This study reviews applicability of the differentspatial analyst techniques in vineyard especially models based on surface relief and the new technology of hyperspectral analysis. Thespectral alteration was examined between different health status and leaves infected by Eriophyes vitis to defi ne spectral characteristics. Thusrefl ectance measurements could also support to evaluate airborne hyperspectral images with the help of the spectral library

Vineyards characteristic by using GIS and refl ectance measurements on the Nagy-Eged hill in Hungary

Our investigation was carried out in vineyards on Nagy-Eged hill in Hungary. Grapevine growing plays an important role in Eger Vine Regions. In the grapevine cultivation it becomes necessary to know the requirements of vineyards along the best geographical situation using available cultivation and examination technology to achieve better and quality produce. This study reviews applicability of the different spatial analyst techniques in vineyard especially models based on surface relief and the new technology of hyperspectral analysis. The spectral alteration was examined between different health status and leaves infected by Eriophyes vitis to defi ne spectral characteristics. Thus reflectance measurements could also support to evaluate airborne hyperspectral images with the help of the spectral library

New perspectives in application of kidney biomarkers in mycotoxin induced nephrotoxicity, with a particular focus on domestic pigs.

The gradual spread of Aspergilli worldwide is adding to the global shortage of food and is affecting its safe consumption. Aspergillus-derived mycotoxins, including aflatoxins and ochratoxin A, and fumonisins (members of the fusariotoxin group) can cause pathological damage to vital organs, including the kidney or liver. Although the kidney functions as the major excretory system in mammals, monitoring and screening for mycotoxin induced nephrotoxicity is only now a developmental area in the field of livestock feed toxicology. Currently the assessment of individual exposure to mycotoxins in man and animals is usually based on the analysis of toxin and/or metabolite contamination in the blood or urine. However, this requires selective and sensitive analytical methods (e.g., HPLC-MS/MS), which are time consuming and expensive. The toxicokinetic of mycotoxin metabolites is becoming better understood. Several kidney biomarkers are used successfully in drug development, however cost-efficient, and reliable kidney biomarkers are urgently needed for monitoring farm animals for early signs of kidney disease. β2-microglobulin (β2-MG) and N-acetyl-β-D-glucosaminidase (NAG) are the dominant biomarkers employed routinely in environmental toxicology research, while kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) are also emerging as effective markers to identify mycotoxin induced nephropathy. Pigs are exposed to mycotoxins due to their cereal-based diet and are particularly susceptible to Aspergillus mycotoxins. In addition to commonly used diagnostic markers for nephrotoxicity including plasma creatinine, NAG, KIM-1 and NGAL can be used in pigs. In this review, the currently available techniques are summarized, which are used for screening mycotoxin induced nephrotoxicity in farm animals. Possible approaches are considered, which could be used to detect mycotoxin induced nephropathy

Nutrigenomic and nutritional analyses reveal the effects of pelleted feeds on Asian Seabass (Lates calcarifer)

As nutrition-related expenses constitute the majority of the costs for aquaculture farms, it is essential for them to use feeds that provide an ideal combination of nutrients for the species of choice. In this study, the relative effect of consuming three different pelleted feeds (B, C and D) in comparison to frozen baitfish (A; control) were compared on juvenile Asian seabass (77.3 ± 22.4g) that were selected for increased growth rate over two generations. Our objectives were: 1) to evaluate the effects of different pelleted feeds based on overall physiological changes and nutritional quality of fillets; 2) improve our understanding of the underlying mechanisms with transcriptomic analysis; 3) if possible, identify the feed type that supports the growth of these fishes without substantially reducing the nutritional quality of fillet. The growth performance, fatty acid composition of fillet, hepatic histology and transcriptome of the fishes (Groups A-D) were analyzed. The majority of fatty acids of the fillets, except γ-linolenic acid (GLA, C18:3n6), correlated significantly with the respective diets. Asian seabass fed Feed C showed highest specific growth rate (SGR) and feed conversion efficiency (FCE) with closest histology and transcriptomic profile to control, but their fillet contained the highest n6/n3 ratio. When the liver-based transcriptomes were analyzed, a complex set of differentially expressed genes were detected between groups fed pelleted feeds and controls as well as among the pellet-fed groups themselves. Significant enrichment of genes with growth-related function tallied with the morphological data measured. When compared with control (Group A), ‘Biosynthesis of unsaturated fatty acids’ and ‘Steroid biosynthesis’ pathways were significantly enriched in pellet-fed groups. Reduced goblet cell numbers were observed in the gut of pellet-fed fish compared to controls and fads6 was found to be a suitable candidate gene to separate wild-caught Asian seabass, from pellet-fed ones. These results provide insights for researchers on the various effects of feeds on the biochemistry and global gene expression of the fish and potentially for seabass farms to make more informed feed choices

Study of the soil—plant (carrot)—animal cycle of nutritive and hazardous minerals in a rabbit model

Carrots were grown on soils polluted by heavy metal salts. Each particular microelement reached a high concentration [molybdenum (Mo) 39.00, cadmium (Cd) 2.30, lead (Pb) 4.01, mercury (Hg) 30.00, and selenium (Se) 36.20 mg/kg dry matter] in the carrot. In a metabolic balance trial conducted with 15 male and 15 female New Zealand White rabbits, the control animals (n = 5) were fed ad libitum with concentrate as basal diet, while the other rabbits received the basal diet and carrots containing the particular microelement. Blood samples were taken to determine the activity of serum enzymes. To investigate the metabolism of Mo, Cd, Pb, Hg and Se, samples were taken from the heart, liver, lungs, kidneys, spleen, ovaries/testicles, entire digestive tract, adipose tissue, femur, hair, faeces and urine. Carrot had significantly higher digestibility for all nutrients than the rabbit concentrate. Carrot samples of high Pb content had the lowest digestibility of crude protein. The microelements differed in their rate of accumulation in the organs examined: Mo and Cd accumulated in the kidneys, Pb in the kidneys, liver, bones and lungs, Hg in the kidneys and liver, while Se in the liver, kidneys and heart. The proportions of microelements eliminated from the body either via the faeces and urine (Mo 80.18% and Se 47.41%) or via the faeces (Cd 37.86%, Pb 66.39%, Hg 64.65%) were determined. Pathohistological examination revealed that the rate of spermatogenesis was reduced in the Mo, Cd, Pb and Hg groups compared to the control. Lead, Cd and Hg intake resulted in a considerable decrease in gamma-glutamyltransferase (GGT) and in an increase of alkaline phosphatase (ALP) activity because of damages to the kidneys and bones. All experimental treatments decreased the activity of cholinesterase (CHE) because of lesions in the liver

Heat stress in dairy cows 2. A review on nutritional strategies to alleviate losses

SUMMARY The present review describes the possible nutritional strategies to alleviate the heat stress related decrease in the milk yield of dairy cows. Heat stress affects the energy metabolism both directly and indirectly. Loss of appetite and reduced feed intake lowers the energy sources available for production, while the increase in basal insulin concentration – as an indirect effect – is determinant in glucose and fatty acid utilization. Nutritional strategies that can lead to improved energy status and moderate increase in production are reviewed in detail. Altering the schedule of feed distribution, supplementing dairy rations with fat or feeding NDF (neutral detergent fibre) components with improved digestibility are effectively increasing energy intake. Yeast supplementation that is widely used in periods of negative energy balance can enhance ruminal fermentation, and it is also effective in heat stress. Niacin supports adaptation to heat stress through several different metabolic pathways, however, studies have come to contradictory conclusions. The enhanced insulin action characteristic to heat stress can be promoted by dietary chromium supplementation and an adjusted ratio of rumen degradable and undegradable protein in the ration. Supply of major cationic and anionic macro elements supports the acid base status and compensates the heat-related electrolyte-deficiency. Other, less investigated supplements, such as betaine, flavonoids and selenium act through mechanisms that are not fully known. The primary goal of feeding solutions is the improvement of the general health status and adaptive capacity of animals which often results in increased production, though understandably to a limited extent. The role of nutritional interventions can thus only be secondary to cooling efforts in preventing heat stress. Increasing knowledge on heat stress physiology can promote the development of nutritional strategies. ÖSSZEFOGLALÁS A szerzők előző, a tejtermelés hőstresszben tapasztalható csökkenését okozó élettani változásokkal kapcsolatos irodalmi áttekintő közleményük folytatásaként az alkalmazkodást segítő takarmányozási megoldásokat mutatják be jelen közleményükben. A fejadag energiatartalmának növelésével, ill. a bendőbeli illózsírsav-termelést serkentő kiegészítőkkel kapcsolatos kutatási eredmények mellett a hőstresszben tapasztalható fokozott inzulinhatást támogató megoldásokat is ismertetik. A sav-bázis egyensúlyt és az ásványianyag-ellátottságot javító, továbbá egyéb, nem pontosan ismert mechanizmus útján ható kiegészítőkkel kapcsolatos eredményeket is összefoglalják

Effect of feeding T-2 toxin contaminated feed on the utilisation of vitamin E in chickens

The purpose of the present study was to investigate the effect of experimental T-2 toxin load (2.35 mg/kg of feed) and vitamin E supply in the drinking water (10.5 mg/bird/day) on vitamin E levels of the blood plasma and liver in broiler chickens in a 14-day experiment. It was found that T-2 toxin load did not influence vitamin E content of the blood plasma except at day 3 after the toxin load when a moderate increase was detected in plasma vitamin E. No significant changes were found in vitamin E content of the liver. The simultaneous use of high-dose vitamin E supplementation and T-2 toxin load caused a significantly higher plasma vitamin E content but the changes were less expressed in the group subjected to T-2 toxin load. Vitamin E supply also resulted in a marked and significant increase in vitamin E concentrations of the liver on days 3 and 7 even in the T-2 loaded group, but this concentration significantly decreased thereafter. The results show that T-2 contamination of the diet has an adverse effect on the utilisation of vitamin E in broiler chickens

Effect of different dietary fat sources on production traits, lipid peroxide status and on the glutathione redox system in African catfish [Clarias gariepinus (Burchell)] fingerlings

Lipids are used to provide the energy to cover the metabolic needs and to provide essential fatty acids, which are important for membrane function [12]. Fats may contain high level of long chain polyunsaturated fatty acids, which are prone to peroxidation [8] and will interact with the antioxidant defense system [1]. There is contradiction in the literature about whether the intake of fish oil enhance [7] or deplete [4] tissue antioxidant defenses and the glutathione redox system in different organisms. The aim of the present study was to examine the effects of different dietary oils on parameters of the lipid peroxide state and the glutathione redox system in C. gariepinus fingerlings