Desempenho produtivo da cultivar de feijão-comum BRS Esteio em Unidades Demonstrativas na Região Centro-Sul do Paraná.

O feijão-comum (Phaseolus vulgaris L.) é o alimento típico da população brasileira. É consumido com o arroz em pelo menos uma das refeições do brasileiro. Do total da produção de feijão da primeira safra, 45,4% provém da região Sul, sendo o Estado do Paraná o maior produtor. Para auxiliar no aumento da produtividade e contribuir para que os agricultores utilizem sementes do sistema formal, a Embrapa desenvolve cultivares de feijão-comum adaptadas às condições de cultivo em todo o país, por exemplo, a cultivar BRS Esteio, do grupo preto. O objetivo deste estudo foi demonstrar o desempenho dessa cultivar no Paraná, utilizando os resultados das Unidades Demonstrativas (UDs) conduzidas pelo Instituto Emater, no escopo do Projeto Centro-Sul de Feijão e Milho, na safra 2014/2015. Foram conduzidos dois tipos de UDs, em áreas de um hectare e de 600 m2, nas quais os dados de produtividade da BRS Esteio foram comparados com os das cultivares do Iapar (IPR). Os resultados indicaram que a BRS Esteio foi superior em rendimento às demais cultivares avaliadas na safra 2014/2015, na maioria dos municípios testados, indicando seu elevado potencial produtivo na região Centro-Sul do Paraná.bitstream/item/160982/1/CNPAF-2017-bpd49.pd

Desempenho produtivo da cultivar de feijão BRS Esteio em unidades demonstrativas na região Centro-Sul do Paraná, 2015/2016.

O objetivo deste estudo foi demonstrar o desempenho da cultivar BRS Esteio no Paraná, utilizando os resultados das Unidades Demonstrativas (UDs) conduzidas pela Emater, no escopo do Projeto Centro-Sul de Feijão e Milho. Em todas as UDs foi aplicado um questionário estruturado onde as variáveis consultadas foram: produtividade (kg ha-1), sistema de plantio (direto ou convencional) e data de plantio

Microbial Translocation Is Associated with Increased Monocyte Activation and Dementia in AIDS Patients

Elevated plasma lipopolysaccharide (LPS), an indicator of microbial translocation from the gut, is a likely cause of systemic immune activation in chronic HIV infection. LPS induces monocyte activation and trafficking into brain, which are key mechanisms in the pathogenesis of HIV-associated dementia (HAD). To determine whether high LPS levels are associated with increased monocyte activation and HAD, we obtained peripheral blood samples from AIDS patients and examined plasma LPS by Limulus amebocyte lysate (LAL) assay, peripheral blood monocytes by FACS, and soluble markers of monocyte activation by ELISA. Purified monocytes were isolated by FACS sorting, and HIV DNA and RNA levels were quantified by real time PCR. Circulating monocytes expressed high levels of the activation markers CD69 and HLA-DR, and harbored low levels of HIV compared to CD4+ T-cells. High plasma LPS levels were associated with increased plasma sCD14 and LPS-binding protein (LBP) levels, and low endotoxin core antibody levels. LPS levels were higher in HAD patients compared to control groups, and were associated with HAD independently of plasma viral load and CD4 counts. LPS levels were higher in AIDS patients using intravenous heroin and/or ethanol, or with Hepatitis C virus (HCV) co-infection, compared to control groups. These results suggest a role for elevated LPS levels in driving monocyte activation in AIDS, thereby contributing to the pathogenesis of HAD, and provide evidence that cofactors linked to substance abuse and HCV co-infection influence these processes

Maize growth in response to Azospirillum brasilense, Rhizobium tropici, molybdenum and nitrogen

ABSTRACT The objective of this research was to evaluate the effect of Azospirillum brasilense, Rhizobium tropici, nitrogen (N) and molybdenum (Mo) fertilization on maize growth. The experiment was carried out in a greenhouse from October to November 2015, in a completely randomized design, in 2 x 2 x 2 x 5 factorial scheme, with 5 replicates, corresponding to the absence and presence of Azospirillum brasilense, Rhizobium tropici, N (30 kg ha-1) and five Mo doses (0, 7.5, 15.0, 22.5 and 30.0 g ha-1). The analyzed variables were: plant height, basal stem diameter, dry biomass of shoots, roots, total and N accumulated in the shoots. There was double or triple interaction between N fertilization, Azospirillum brasilense and Rhizobium tropici for the evaluated variables. However, isolated and/or combined effect of Mo was not observed. Seed inoculation with Azospirillum brasilense as well as their co-inoculation with Rhizobium tropici in the absence of N fertilization was efficient to increase plant growth. Soil N fertilization at sowing was less efficient in promoting plant growth than when it was combined with seed inoculation with Rhizobium tropici

Produção de mudas de alface com o uso de substrato preparado com coprólitos de minhoca Prodution of seedlings of lettuce with the use of prepared substrate with earthworm casting

Com o objetivo de avaliar o efeito de coprólitos de minhoca (Chibui bari Righi & Guerra, 1985) na produção de mudas de alface (Lactuca sativa L.) cv. Simpson, realizaram-se dois experimentos em casa de vegetação na Universidade Federal do Acre, ambos no delineamento experimental inteiramente casualizado, com seis tratamentos e seis repetições, totalizando 36 unidades experimentais, representadas por copos plásticos de 180 cm³. Os tratamentos foram definidos a partir da mistura de seis concentrações (0, 20, 40, 60, 80 e 100%) de solo e coprólitos. Utilizaram-se dois tipos de solo, eutrófico no experimento 1 e distrófico no experimento 2. Aos 34 dias após a semeadura avaliaram-se a matéria seca da parte aérea, da raiz e total da planta. Os resultados do experimento 1 indicam que o acréscimo de coprólitos a solos eutróficos promove máximo incremento de massa seca da parte aérea e total da planta com concentrações de coprólitos de 13,49% e 17,88%, respectivamente. Entretanto, os resultados do experimento 2 indicam que a adição de coprólitos a solos distróficos, responde a uma função cúbica, com máximo incremento de massa seca da raiz e total da planta com concentrações de coprólitos de 17,49% e 21,40%, respectivamente, com decréscimo em 68,23% e 67,48%, e incremento máximo novamente com 100% de coprólitos para ambas características. O uso de substratos preparados com solo e coprólitos de minhoca somente contribui para o crescimento de mudas de alface se a condição química dos coprólitos for mais adequada que a do solo em atender as necessidades nutricionais das plantas.<br>With the aim of evaluating the effect of earthworm casting (Chibui bari Righi & Guerra, 1985) in the production of lettuce seedlings (Lactuca sativa L.) cv. Simpson, two experiments ware carried out in greenhouse in the Federal University of the Acre, both in a completely randomized outline with six treatments and six repetitions, totalizing 36 experimental units, represented by 180 cm³ plastic cups. The treatments were defined from the mixture of six concentrations (0, 20, 40, 60, 80 and 100%) of soil and earthworm casting. Two soil types were used, eutrophic in the experiment 1, and dystrophic in experiment 2. At 34 days after the sowing the dry mass of shoot, root and total plant were evaluated. The results of experiment 1 indicate that the addition the earthworm casting in eutrophic soil promotes increase of dry mass of the aerial and total fast of the plant concentrations of earthworm casting 17.88% and 13.49%, respectively. However, the results of experiment 2 indicate that the addition of the earthworm casting to the dystrophic soil, responds to a cubic function, with maximum dry mass total and of root increase with concentrations of earthworm casting of 17.49% and 21.40%, respectively, with decrease in 68.23% and 67.48%, and increase maximum again with 100% of earthworm casting for both characteristics. The use of substrate prepared with soil and earthworm casting only contributes to the growth of lettuce seedlings if the chemical condition of the casting is more appropriate than the one of the soil in assisting the nutritious needs of the plants

Chemical-genetic analysis of cyclin dependent kinase 2 function reveals an important role in cellular transformation by multiple oncogenic pathways

A family of conserved serine/threonine kinases known as cyclin-dependent kinases (CDKs) drives orderly cell cycle progression in mammalian cells. Prior studies have suggested that CDK2 regulates S-phase entry and progression, and frequently shows increased activity in a wide spectrum of human tumors. Genetic KO/knockdown approaches, however, have suggested that lack of CDK2 protein does not prevent cellular proliferation, both during somatic development in mice as well as in human cancer cell lines. Here, we use an alternative, chemical-genetic approach to achieve specific inhibition of CDK2 kinase activity in cells. We directly compare small-molecule inhibition of CDK2 kinase activity with siRNA knockdown and show that small-molecule inhibition results in marked defects in proliferation of nontransformed cells, whereas siRNA knockdown does not, highlighting the differences between these two approaches. In addition, CDK2 inhibition drastically diminishes anchorage-independent growth of human cancer cells and cells transformed with various oncogenes. Our results establish that CDK2 activity is necessary for normal mammalian cell cycle progression and suggest that it might be a useful therapeutic target for treating cancer