SLIDES: Wrapping Up the Big Horn Adjudication: Lessons After 38 Years and 20,000 Claims

Presenter: Ramsey L. Kropf, Deputy Solicitor for Water Resources, Office of the Solicitor, U.S. Department of the Interior 34 slide

Basin-Wide Adjudications in the West: What Works, What Doesn’t?

18 pages. Contains 2 pages of references

MF2313

Donald Kropf & Curtis Kastner, Color and oxidative properties of irradiated beef, Kansas State University, January 1998

Life Expectancy: Social Work with Centenarians

Although the older population as a whole is increasing faster than any other age group, the most dramatic growth is in the oldest old. Centenarians, those individuals who have survived 100 or more years, have increased ten times in size over the past forty years. This population trajectory is expected to accelerate even more into the next century. Unfomately, social work with the older population rarely includes practice issues related to work with these older adults who have survived well past the average lie expectancy. This article provides a description of the current cohort of centenarians from a biopsychosocial framework and presents an agenda for social work practice, policy and research

Alignment of Centrosomal and Growth Axes Is a Late Event during Polarization ofPelvetia compressaZygotes

AbstractZygotes and embryos of the fucoid brown algaPelvetia compressaundergo a series of asymmetric cleavages. We are interested in the developmental role of these cleavages and the mechanism controlling their alignment. To assess the importance of division plane alignment, the orientation of the first asymmetric division was altered and the effects on subsequent embryo elongation rates were analyzed. Although this division is normally oriented transversely (90°) to the growth axis, deviations up to 45° had no significant effects on embryo elongation. However, division planes that were parallel with the growth axis (0–45°) had drastic effects. Embryo elongation was severely inhibited and the wall often bifurcated and avoided the rhizoid tip. The orientation of the division plane is determined by the position of the centrosomes. We therefore investigated centrosomal position and function during the first cell cycle within the three-dimensional context of the cell. We found that, after karyogamy, microtubule organization changed from a radially symmetric circumnuclear array into a bipolar centrosomal array. The reorganization coincided with the migration of the centrosomes around the nucleus. The centrosomes separated slowly and asynchronously until they reached opposite sides of the nuclear envelope. At this time the centrosomal axis, defined by the position of the two centrosomes, was oriented randomly with respect to the cortical growth axis. The centrosomal axis then rotated into alignment parallel with the growth axis late in the first cell cycle. These results indicate that the growth axis and the centrosomal axis develop independently of each other and that the centrosomal axis does not align with the growth axis until just prior to mitosis

Kinesin-5 motors are required for organization of spindle microtubules in Silvetia compressa zygotes

BACKGROUND: Monastrol, a chemical inhibitor specific to the Kinesin-5 family of motor proteins, was used to examine the functional roles of Kinesin-5 proteins during the first, asymmetric cell division cycle in the brown alga Silvetia compressa. RESULTS: Monastrol treatment had no effect on developing zygotes prior to entry into mitosis. After mitosis entry, monastrol treatment led to formation of monasters and cell cycle arrest in a dose dependent fashion. These findings indicate that Kinesin-5 motors maintain spindle bipolarity, and are consistent with reports in animal cells. At low drug concentrations that permitted cell division, spindle position was highly displaced from normal, resulting in abnormal division planes. Strikingly, application of monastrol also led to formation of numerous cytasters throughout the cytoplasm and multipolar spindles, uncovering a novel effect of monastrol treatment not observed in animal cells. CONCLUSION: We postulate that monastrol treatment causes spindle poles to break apart forming cytasters, some of which capture chromosomes and become supernumerary spindle poles. Thus, in addition to maintaining spindle bipolarity, Kinesin-5 members in S. compressa likely organize microtubules at spindle poles. To our knowledge, this is the first functional characterization of the Kinesin-5 family in stramenopiles

Polarization of the endomembrane system is an early event in fucoid zygote development

BACKGROUND: Fucoid zygotes are excellent experimental organisms for investigating mechanisms that establish cell polarity and determine the site of tip growth. A common feature of polarity establishment is targeting endocytosis and exocytosis (secretion) to localized cortical domains. We have investigated the spatiotemporal development of endomembrane asymmetry in photopolarizing zygotes, and examined the underlying cellular physiology. RESULTS: The vital dye FM4-64 was used to visualize endomembranes. The endomembrane system preferentially accumulated at the rhizoid (growth) pole within 4 h of fertilization. The polarized endomembrane array was initially labile and reoriented when the developmental axis changed direction in response to changing light cues. Pharmacological studies indicated that vesicle trafficking, actin and microtubules were needed to maintain endomembrane polarity. In addition, endocytosis required a functional cortical actin cytoskeleton. CONCLUSION: Endomembrane polarization is an early event in polarity establishment, beginning very soon after photolocalization of cortical actin to the presumptive rhizoid site. Targeting of endocytosis and secretion to the rhizoid cortex contributes to membrane asymmetry. We suggest that microtubule-actin interactions, possibly involving microtubule capture and stabilization at actin-rich sites in the rhizoid, may organize the endomembrane array

Knowledge of and Attitudes toward Aging among Non-elders: Gender and Race Differences

Although the aging process begins at birth, fears about late adulthood can foster anxiety in younger cohorts about this time of life. This study examines the relationship between non-elderly subjects\u27 (n = 884, 18-55 years) knowledge of and anxieties regarding personal aging and their gender and race. We hypothesized that nonelderly women and persons of color, those who will experience multiple jeopardy in their own late life, would report greater anxiety about their own aging process than did men and majority group members. Women did report lower income and education levels, less knowledge of aging, greater anxiety related to their own aging process, and more time involved in caregiving activities. People of color also differed from Caucasians in certain dimensions of knowledge and anxiety. Implications from these results include the creation of public educational strategies and the organization of women and minorities to more actively address the development and shape of age-related policies

Co-transport-induced instability of membrane voltage in tip-growing cells

A salient feature of stationary patterns in tip-growing cells is the key role played by the symports and antiports, membrane proteins that translocate two ionic species at the same time. It is shown that these co-transporters destabilize generically the membrane voltage if the two translocated ions diffuse differently and carry a charge of opposite (same) sign for symports (antiports). Orders of magnitude obtained for the time and lengthscale are in agreement with experiments. A weakly nonlinear analysis characterizes the bifurcation

The role of neutrophils in pregnancy, term and preterm labour

Neutrophils are surveillance cells, and the first to react and migrate to sites of inflammation and infection following a chemotactic gradient. Neutrophils play a key role in both sterile inflammation and infection, performing a wide variety of effector functions such as degranulation, phagocytosis, ROS production and release of neutrophil extracellular traps (NETs). Healthy term labour requires a sterile pro-inflammatory process, whereas one of the most common causes of spontaneous preterm birth is microbial driven. Peripheral neutrophilia has long been described during pregnancy, and evidence exists demonstrating neutrophils infiltrating the cervix, uterus and foetal membranes during both term and preterm deliveries. Their presence supports a role in tissue remodelling via their effector functions. In this review, we describe the effector functions of neutrophils. We summarise the evidence to support their role in healthy pregnancy and labour and describe their potential contribution to microbial driven preterm birth