Dynamics of the formation of a hydrogel by a pathogenic amyloid peptide: islet amyloid polypeptide

Many chronic degenerative diseases result from aggregation of misfolded polypeptides to form amyloids. Many amyloidogenic polypeptides are surfactants and their assembly can be catalysed by hydrophobic-hydrophilic interfaces (an air-water interface in-vitro or membranes in-vivo). We recently demonstrated the specificity of surface-induced amyloidogenesis but the mechanisms of amyloidogenesis and more specifically of adsorption at hydrophobic-hydrophilic interfaces remain poorly understood. Thus, it is critical to determine how amyloidogenic polypeptides behave at interfaces. Here we used surface tensiometry, rheology and electron microscopy to demonstrate the complex dynamics of gelation by full-length human islet amyloid polypeptide (involved in type II diabetes) both in the bulk solution and at hydrophobic-hydrophilic interfaces (air-water interface and phospholipids). We show that the hydrogel consists of a 3D supramolecular network of fibrils. We also assessed the role of solvation and dissected the evolution over time of the assembly processes. Amyloid gelation could have important pathological consequences for membrane integrity and cellular functions

Aerodynamics of lift fan V/STOL aircraft

Aerodynamic characteristics of lift fan installation for direct lift V/STOL aircraf

Building Collaborative Research to Drive Improvement of West Virginia Health Outcomes

Mortality rates in Appalachia have not declined in recent years as they have for the remainder of the US. Appalachian mortality rates have actually increased. Most counties of southern West Virginia have mortality rates well in excess of the US average. West Virginia ranks at or near the bottom in most U.S. chronic disease categories, with the highest rate of drug overdose deaths and near the highest rates of cancer and cardiovascular mortality. And yet, West Virginia has many dedicated heath care providers, committed to the care of their patients. The West Virginia Clinical and Translational Science Institute (WVCTSI) seeks to facilitate finding solutions for vexing health problems in West Virginia, working with the many committed healthcare providers to collaboratively improve health outcomes in our state

Hiv acquisition among women from selected areas of the united states a cohort study

Women account for 23% of newly diagnosed HIV infections in the United States, but there are few recent, well-characterized cohorts of U.S. women in whom behavior characteristics and HIV acquisition have been well-described

AIDS in America — Forgotten but Not Gone

Over the past decade, limited attention has been paid to the human immunodeficiency virus (HIV) epidemic in the United States. The global epidemic — particularly the epidemic in sub-Saharan Africa, where approximately two thirds of the world's population living with AIDS resides — has rightfully received most of the focus. Meanwhile, however, the prevalence of HIV infection within some U.S. populations now rivals that in some sub-Saharan African countries

Aerodynamic characteristics of a large-scale V/STOL transport model with tandem lift fans mounted at mid-semispan of the wing

Aerodynamic characteristics of V/STOL transport model with tandem lift fans mounted at mid-semispan of win

Higher comfort temperature preferences for anthropometrically matched Chinese and Japanese versus White-Western-Middle-European individuals using a personal comfort / cooling system

Purpose: To investigate potential differences in preferred Personal Comfort Systems (PCS) settings of Japanese and Han-Chinese versus white-western-middle-Europeans. Method: A series of five experiments with similar methodology is reported that allowed participants to self-select their preferred PCS outlet air temperature in a warm controlled climatic chamber setup with and without solar radiation. Test groups were matched for age, height, weight, body-surface-area and body-mass-index to remove the influence of these confounding factors on the results. Participants were first exposed to solar radiation (exp-1-4; simulating glazed building without proper shading or a car) before starting to control the outlet temperature of the PCS, or (exp-5, simulating warm building) were exposed to a warm room temperature and immediately could control the PCS. Ethnicity effects were studied through the chosen preferred PCS outlet temperatures and the microclimate temperature close to the participants’ chest. Results: In all experiments, Asian groups selected a PCS outlet temperature significantly higher, on average by 5 °C, leading to a 1.9 °C higher microclimate temperature at chest level. While absolute selected temperatures of the PCS differed between experiments, related to different designs of the PCS and climate conditions, no interaction between ethnicity and experiment was present. Conclusions: Despite removing important confounding factors that could explain earlier observed differences between Asian and white western middle-European ethnicities tested, a substantial, consistently higher thermal preference temperature of the PCS was found in the two Asian groups. This has implications for the design parameters of PCS for use in offices or air-conditioning systems in cars

Causal mechanisms in the clinical course and treatment of back pain

In recent years, there has been increasing interest in studying causal mechanisms in the development and treatment of back pain. The aim of this article is to provide an overview of our current understanding of causal mechanisms in the field. In the first section, we introduce key concepts and terminology. In the second section, we provide a brief synopsis of systematic reviews of mechanism studies relevant to the clinical course and treatment of back pain. In the third section, we reflect on the findings of our review to explain how understanding causal mechanisms can inform clinical practice and the implementation of best practice. In the final sections, we introduce contemporary methodological advances, highlight the key assumptions of these methods, and discuss future directions to advance the quality of mechanism-related studies in the back pain field

The SKA Particle Array Prototype: The First Particle Detector at the Murchison Radio-astronomy Observatory

We report on the design, deployment, and first results from a scintillation detector deployed at the Murchison Radio-astronomy Observatory (MRO). The detector is a prototype for a larger array -- the Square Kilometre Array Particle Array (SKAPA) -- planned to allow the radio-detection of cosmic rays with the Murchison Widefield Array and the low-frequency component of the Square Kilometre Array. The prototype design has been driven by stringent limits on radio emissions at the MRO, and to ensure survivability in a desert environment. Using data taken from Nov.\ 2018 to Feb.\ 2019, we characterize the detector response while accounting for the effects of temperature fluctuations, and calibrate the sensitivity of the prototype detector to through-going muons. This verifies the feasibility of cosmic ray detection at the MRO. We then estimate the required parameters of a planned array of eight such detectors to be used to trigger radio observations by the Murchison Widefield Array.Comment: 17 pages, 14 figures, 3 table