Packing defects and the width of biopolymer bundles

The formation of bundles composed of actin filaments and cross-linking proteins is an essential process in the maintenance of the cells' cytoskeleton. It has also been recreated by in-vitro experiments, where actin networks are routinely produced to mimic and study the cellular structures. It has long been observed that these bundles seem to have a well defined width distribution, which has not been adequately described theoretically. We propose here that packing defects of the filaments, quenched and random, contribute an effective repulsion that counters the cross-linking adhesion energy and leads to a well defined bundle width. This is a two-dimensional strain-field version of the classic Rayleigh instability of charged droplets

Bcc 4^4He as a Coherent Quantum Solid

In this work we investigate implications of the quantum nature of bcc $^{4}$% He. We show that it is a unique solid phase with both a lattice structure and an Off-Diagonal Long Range Order of coherently oscillating local electric dipole moments. These dipoles arise from the local motion of the atoms in the crystal potential well, and oscillate in synchrony to reduce the dipolar interaction energy. The dipolar ground-state is therefore found to be a coherent state with a well defined global phase and a three-component complex order parameter. The condensation energy of the dipoles in the bcc phase stabilizes it over the hcp phase at finite temperatures. We further show that there can be fermionic excitations of this ground-state and predict that they form an optical-like branch in the (110) direction. A comparison with 'super-solid' models is also discussed.Comment: 12 pages, 8 figure

Evaporative Cooling of a Guided Rubidium Atomic Beam

We report on our recent progress in the manipulation and cooling of a magnetically guided, high flux beam of $^{87}{\rm Rb}$ atoms. Typically $7\times 10^9$ atoms per second propagate in a magnetic guide providing a transverse gradient of 800 G/cm, with a temperature $\sim550$ $\mu$K, at an initial velocity of 90 cm/s. The atoms are subsequently slowed down to $\sim 60$ cm/s using an upward slope. The relatively high collision rate (5 s$^{-1}$) allows us to start forced evaporative cooling of the beam, leading to a reduction of the beam temperature by a factor of ~4, and a ten-fold increase of the on-axis phase-space density.Comment: 10 pages, 8 figure

Force balance and membrane shedding at the Red Blood Cell surface

During the aging of the red-blood cell, or under conditions of extreme echinocytosis, membrane is shed from the cell plasma membrane in the form of nano-vesicles. We propose that this process is the result of the self-adaptation of the membrane surface area to the elastic stress imposed by the spectrin cytoskeleton, via the local buckling of membrane under increasing cytoskeleton stiffness. This model introduces the concept of force balance as a regulatory process at the cell membrane, and quantitatively reproduces the rate of area loss in aging red-blood cells.Comment: 4 pages, 3 figure

Ferromagnetism of 3^3He Films in the Low Field Limit

We provide evidence for a finite temperature ferromagnetic transition in 2-dimensions as $H \to 0$ in thin films of $^3$He on graphite, a model system for the study of two-dimensional magnetism. We perform pulsed and CW NMR experiments at fields of 0.03 - 0.48 mT on $^3$He at areal densities of 20.5 - 24.2 atoms/nm$^2$. At these densities, the second layer of $^3$He has a strongly ferromagnetic tendency. With decreasing temperature, we find a rapid onset of magnetization that becomes independent of the applied field at temperatures in the vicinity of 1 mK. Both the dipolar field and the NMR linewidth grow rapidly as well, which is consistent with a large (order unity) polarization of the $^3$He spins.Comment: 4 figure

Propagating Cell-Membrane Waves Driven by Curved Activators of Actin Polymerization

Cells exhibit propagating membrane waves which involve the actin cytoskeleton. One type of such membranal waves are Circular Dorsal Ruffles (CDR) which are related to endocytosis and receptor internalization. Experimentally, CDRs have been associated with membrane bound activators of actin polymerization of concave shape. We present experimental evidence for the localization of convex membrane proteins in these structures, and their insensitivity to inhibition of myosin II contractility in immortalized mouse embryo fibroblasts cell cultures. These observations lead us to propose a theoretical model which explains the formation of these waves due to the interplay between complexes that contain activators of actin polymerization and membrane-bound curved proteins of both types of curvature (concave and convex). Our model predicts that the activity of both types of curved proteins is essential for sustaining propagating waves, which are abolished when one type of curved activator is removed. Within this model waves are initiated when the level of actin polymerization induced by the curved activators is higher than some threshold value, which allows the cell to control CDR formation. We demonstrate that the model can explain many features of CDRs, and give several testable predictions. This work demonstrates the importance of curved membrane proteins in organizing the actin cytoskeleton and cell shape

Improvements to Response-Surface Based Vehicle Design Using a Feature-Centric Approach

Abstract. In this paper, we present our vision for a framework to facilitate computationally-based aerospace vehicle design by improving the quality of the response surfaces that can be developed for a given cost. The response surfaces are developed using computational fluid dynamics (CFD) techniques of varying fidelity. We propose to improve the quality of a given response surface by exploiting the relationships between the response surface and the flow features that evolve in response to changes in the design parameters. The underlying technology, generalized feature mining, is employed to locate and characterize features as well as provide explanations for feature-feature and feature-vehicle interactions. We briefly describe the components of our framework and outline two different strategies to improve the quality of a response surface. We also highlight ongoing efforts

Optimizing the Design of Spacecraft Systems Using Risk as Currency

Abstract-Treating risk as a "currency" has proven to be key in systematically optimizing the design of spacecraft systems. This idea has been applied in the design of individual components of spacecraft systems, and in the end-to-end design of such systems. The process, called "Defect Detection and Prevention" (DDP), its tool support, and applications, are described in We are now extending this process to include consideration of architectural alternatives, qualification of components, fabrication and assembly, integration and test, and mission operation. The results of applying this extended process in the pre-formulation, formulation and implementation phases of various NASA and other government agency missions will be discussed. This paper will also discuss the results of developing optimized technology development and qualification plans

Atomic micromotion and geometric forces in a triaxial magnetic trap

Non-adiabatic motion of Bose-Einstein condensates of rubidium atoms arising from the dynamical nature of a time-orbiting-potential (TOP) trap was observed experimentally. The orbital micromotion of the condensate in velocity space at the frequency of the rotating bias field of the TOP was detected by a time-of-flight method. A dependence of the equilibrium position of the atoms on the sense of rotation of the bias field was observed. We have compared our experimental findings with numerical simulations. The nonadiabatic following of the atomic spin in the trap rotating magnetic field produces geometric forces acting on the trapped atoms.Comment: 4 pages, 4 figure

Fluctuations in active membranes

Active contributions to fluctuations are a direct consequence of metabolic energy consumption in living cells. Such metabolic processes continuously create active forces, which deform the membrane to control motility, proliferation as well as homeostasis. Membrane fluctuations contain therefore valuable information on the nature of active forces, but classical analysis of membrane fluctuations has been primarily centered on purely thermal driving. This chapter provides an overview of relevant experimental and theoretical approaches to measure, analyze and model active membrane fluctuations. In the focus of the discussion remains the intrinsic problem that the sole fluctuation analysis may not be sufficient to separate active from thermal contributions, since the presence of activity may modify membrane mechanical properties themselves. By combining independent measurements of spontaneous fluctuations and mechanical response, it is possible to directly quantify time and energy-scales of the active contributions, allowing for a refinement of current theoretical descriptions of active membranes.Comment: 38 pages, 9 figures, book chapte