Use of Distress and Depression Thermometers to Measure Psychosocial Morbidity Among Southern European Cancer Patients

GOALS OF WORK: Recent literature has indicated the need for rapid evaluation of psychosocial issues secondary to cancer. Because of the problems of routine use of psychometric instruments, short instruments such as visual analogue scales or one-item 0-10 scales have been developed as valid assessment alternatives. PATIENTS AND METHODS: A study was conducted to examine the role of two 0-10 scales in measuring emotional stress (distress thermometer, DT) and depressed mood (mood thermometer, MT), respectively, in a multicenter study carried out in southern European countries (Italy, Portugal, Spain, and Switzerland). A convenience sample of 312 cancer outpatients completed the DT and MT and the Hospital Anxiety Depression Scale (HADS). MAIN RESULTS: DT was more significantly associated HADS anxiety than HADS depression while MT was related both to HADS anxiety and depression. The correlation of MT with HADS was higher than DT. A cutoff point >4 on the DT maximized sensitivity (65%) and specificity (79%) for general psychosocial morbidity while a cutoff >5 identified more severe "caseness" (sensitivity=70%; specificity=73%). On the MT, sensitivity and specificity for general psychosocial morbidity were 85% and 72% by using the cutoff score >3. A score >4 on the MT was associated with a sensitivity of 78% and a specificity of 77% in detecting more severe caseness. CONCLUSIONS: Two simple instruments, the DT and the MT, were found to have acceptable levels of sensitivity and specificity in detecting psychosocial morbidity. Compared to the HADS, however, the mood MT performed better than the DT

Performances of multi-gap timing RPCs for relativistic ions in the range Z=1-6

We present the performance of Multi-gap timing RPCs under irradiation by fully stripped relativistic ions (gamma*beta=2.7, Z=1-6). A time resolution of 80 ps at high efficiency has been obtained by just using standard `off the shelf' 4-gap timing RPCs from the new HADES ToF wall. The resolution worsened to 100 ps for ~ 1 kHz/cm2 proton flux and for ~ 100 Hz/cm2 Carbon flux. The chambers were operated at a standard field of E=100 kV/cm and showed a high stability during the experiment, supporting the fact that RPCs are a convenient choice when accommodating a very broad range of ionizing particles is needed. The data provides insight in the region of very highly ionizing particles (up to x 36 mips) and can be used to constrain the existing avalanche and Space-Charge models far from the usual `mip valley'. The implications of these results for the general case of detection based on secondary processes (n, gamma) resulting in highly ionizing particles with characteristic energy distributions will be discussed, together with the nature of the time-charge correlation curve.Comment: 31 pages, 19 figures, submitted to JINS

Long-term preservation of freeze-dried rabbit sperm by adding rosmarinic acid and different chelating agents

Freeze-drying (FD) technique has been applied as an alternative technology to preserve gene resources to allow simple sperm preservation and shipment at 4 degrees C. Nevertheless, DNA sperm might be damaged by mechanical or oxidative stress throughout FD procedure. Therefore, suitable protection to maintain DNA integrity is required. The aim of this study was to determine the effect of rosmarinic acid (RA) as an antioxidant and two chelating agents (EGTA and EDTA) on the DNA integrity of freeze-dried rabbit sperm after storage of the samples at 4 degrees C and room temperature for 8 months. Rabbit sperm were freeze-dried in basic medium (10 mM Tris-HCl buffer and 50 mM NaCl) supplemented with 50 mM EGTA (1), 50 mM EGTA plus 105 mu M RA (2), 50 mM EDTA (3) or 50 mM EDTA plus 105 mu M RA (4). Semen samples were kept at 4 degrees C and room temperature during 8 months. After rehydration, DNA integrity was evaluated with Sperm Chromatin Dispersion test observing that DNA fragmentation was higher when semen samples were freeze-dried with EGTA (10.9%) than with EDTA (4.1%) (p < 0.01). Furthermore, RA acted better under adverse conditions and no significant differences were found in temperature storage. Summarizing, FD is a method that can allow simple gene resources preservation among 4 degrees C to 25 degrees C during 8 months and transportation without the need for liquid nitrogen or dry ice. EDTA chelating agent is the most suitable media for freeze-dried rabbit sperm and the addition of RA protects the DNA against the oxidative stress caused during FD procedure

SWI/SNF regulates a transcriptional programme that induces senescence to prevent liver cancer

Oncogene-induced senescence (OIS) is a potent tumour suppressor mechanism. To identify senescence regulators relevant to cancer, we screened an shRNA library targeting genes deleted in hepatocellular carcinoma (HCC). Here, we describe how knockdown of the SWI/SNF component ARID1B prevents OIS and cooperates with RAS to induce liver tumours. ARID1B controls p16INK4a and p21CIP1a transcription but also regulates DNA damage, oxidative stress and p53 induction, suggesting that SWI/SNF uses additional mechanisms to regulate senescence. To systematically identify SWI/SNF targets regulating senescence, we carried out a focused shRNA screen. We discovered several new senescence regulators including ENTPD7, an enzyme that hydrolyses nucleotides. ENTPD7 affects oxidative stress, DNA damage and senescence. Importantly, expression of ENTPD7 or inhibition of nucleotide synthesis in ARID1B-depleted cells results in re-establishment of senescence. Our results identify novel mechanisms by which epigenetic regulators can affect tumor progression and suggest that pro-senescence therapies could be employed against SWI/SNF-mutated cancers

New VR magnification ratios of QSO 0957+561

We present VR magnification ratios of QSO 0957+561, which are inferred from the GLITP light curves of Q0957+561A and new frames taken with the 2.56m Nordic Optical Telescope about 14 months after the GLITP monitoring. From two photometric approaches and a reasonable range for the time delay in the system (415-430 days), we do not obtain achromatic optical continuum ratios, but ratios depending on the wavelength. These new measurements are consistent with differential extinction in the lens galaxy, the Lyman limit system, the damped Ly-alpha system, or the host galaxy of the QSO. The possible values for the differential extinction and the ratio of total to selective extinction in the V band are reasonable. Moreover, crude probability arguments suggest that the ray paths of the two components cross a similar dusty environment, including a network of compact dust clouds and compact dust voids. As an alternative (in fact, the usual interpretation of the old ratios), we also try to explain the new ratios as caused by gravitational microlensing in the deflector. From magnification maps for each of the gravitationally lensed images, using different fractions of the surface mass density represented by the microlenses, as well as different sizes and profiles of the V-band and R-band sources, several synthetic distributions of V-band and R-band ratios are derived. In some gravitational scenarios, there is an apparent disagreement between the observed pair of ratios and the simulated distributions. However, several microlensing pictures work well. To decide between either extinction, or microlensing, or a mixed scenario (extinction + microlensing), new observational and interpretation efforts are required.Comment: PS and PDF versions are created from the LaTeX file and 5 EPS figures, two additional figues (Figs. 6 and 7) in JPEG format, scheduled for the ApJ 20 January 2005 issu

Brentwood Community Health Care Assessment

BACKGROUND: The Community Alliance for Research Empowering Social Change (CARES) is an academic–community research partnership designed to train community members on research methods and develop the infrastructure for community-based participatory research (CBPR) to examine and address racial/ethnic health disparities. The Brentwood Community Health Assessment (BCHA) was developed through a CBPR pilot project grant from CARES. OBJECTIVES: The purpose of the BCHA is to assess health care utilization and identify existing barriers to health care access among a multi-ethnic community in the Hamlet of Brentwood, New York. METHODS: Using CBPR approaches, the community–academic research partnership develop the study design and survey instrument. Trained Bilingual (English/Spanish) data collectors verbally administered surveys door-to-door to residents of Brentwood from October 2010 to May 2011. Inclusion criteria required participants to be at least 18 years of age and speak either English or Spanish. RESULTS: Overall, 232 residents completed the BCHA; 49% were male, 66% Hispanic, 13% non-Hispanic White, 13% non-Hispanic Black, 29% had less than a high school education, and 33% were born in United States. The assessment results revealed that most residents are able to access health care when needed and the most significant barriers to health care access are insurance and cost. CONCLUSIONS: We describe the community–academic partnered process used to develop and implement the BCHA and report assessment findings; the community-partnered approach improved data collection and allowed access into one of Suffolk County’s most vulnerable communities

Thermal Effect on TL Response of Single Doped LiF+NaF:RE Polycrystalline Phosphors

In this work, the sintering and annealing effects on the thermoluminescent (TL) behavior of undoped and rare earth (RE)-doped LiF+NaF powder samples (RE = Ce3+, Eu3+, Lu3+ or Tl+, at 0.5 mol%) was analyzed by evaluating the sensitivity to gamma radiation and TL response of the material. The polycrystalline samples were obtained by solid state reaction at 1000°C. The samples were irradiated in a Gammacell-3000 Elan irradiator loaded with 137Cs sources. The glow curves of the LiF+NaF doped with lutetium or thallium show an intense glow peak at about 175°C and 135°C, respectively. When the phosphor was doped with cerium or europium the glow curves were complex in their structure, with TL peaks observed at 155°C and 165°C, respectively. The linear dose-response was between 10 and 50 Gy for cerium, europium or lutetium doped LiF+NaF samples, while for the thallium doped and undoped samples such intervals were 10-100 Gy and 10-500 Gy, respectively. Because the shape of the glow curves were complex, the analysis was carried out in (i) samples without a sintering treatment where the TL response was found insensitive to pre-irradiation annealing treatment, and (ii) sintered samples (300, 350, 400 or 500 °C), in this last case the TL response was dependent on the annealing temperature (100-400 °C), finally (iii) the kinetics parameters of the glow curves were analyzed by assuming a general order kinetics model. The observed glow curves and TL characteristics of the LiF+NaF:RE phosphor make attractive this material to be useful in gamma dose dosimetry

Coral Reefs in the Gulf of Mexico Large Marine Ecosystem: Conservation Status, Challenges, and Opportunities

The importance of coral reefs (CR) within marine ecosystems has become widely recognized. Although shallow CR are not as abundant in the Gulf of Mexico (GoM) as in other areas such as the Caribbean, their uniqueness, singularity, isolation, and conservation status make their conservation highly important. Corals and CR, both shallow and deep, are more widely distributed throughout the GoM than previously thought, providing new venues of research but also new challenges for their sustainable management. They are widely present in the three countries circumscribing the GoM (Cuba, Mexico, and the United States). Corals are also distributed throughout different depths, from the keys of Florida and Cuba, to the mesophotic reefs in Flower Garden Banks, Pulley Ridge, and submerged banks in the southern GoM; additional coral presence occurs even beyond mesophotic depths (~30–150 m). Like reefs around the world, they are subject to an increased threat from anthropogenic causes, including overfishing, pollution, and climate change. But there is also hope. Some reefs in the area, such as those in Flower Garden Banks National Marine Sanctuary are probably the best-preserved reefs in the region, with coral cover greater than 50%, which is unusual in the Wider Caribbean. Others are experiencing new protections through the work of government, and local communities. The objectives of this manuscript are to summarize the overall status of corals and CR in the GoM, analyze some of the current and future threats, and explore opportunities for their conservation in the region. Aside from the above mentioned anthropogenic threats bleaching, coral diseases, and hurricanes have been identified as main contributors for CR declines not only in the GoM but abroad; some nowadays present but likely to increase threats are invasion by alien species or by Sargassum spp. Among some of the opportunities identified are to capitalize on existing and emerging multilateral agreements, and initiatives (e.g., GoM Large Marine Ecosystem, trinational sanctuaries agreement); increase financial support for conservation through international initiatives and the private sector; and a need to comprehend the inherent interconnection among corals, CR, and deeper bank ecosystems as they do not function in isolation

Analisi scientifiche sulle tempere murali di Villa Pace

International audienceThe morphology, mineralogy, and solid-liquid phase separation of the Cu and Zn precipitates formed with sulfide produced in a sulfate-reducing bioreactor were studied at pH 3, 5, and 7. The precipitates formed at pH 7 display faster settling rates, better dewaterability, and higher concentrations of settleable solids as compared to the precipitates formed at pH 3 and 5. These differences were linked to the agglomeration of the sulfidic precipitates and coprecipitation of the phosphate added to the bioreactor influent. The Cu and Zn quenched the intensity of the dissolved organic matter peaks identified by fluorescence-excitation emission matrix spectroscopy, suggesting a binding mechanism that decreases supersaturation, especially at pH 5. X-ray absorption fine structure spectroscopy analyses confirmed the precipitation of Zn-S as sphalerite and Cu-S as covellite in all samples, but also revealed the presence of Zn sorbed on hydroxyapatite. These analyses further showed that CuS structures remained amorphous regardless of the pH, whereas the ZnS structure was more organized at pH 5 as compared to the ZnS formed at pH 3 and 7, in agreement with the cubic sphalerite-type structures observed through scanning electron microscopy at pH 5

Multiplexed immunosensors for point-of-care diagnostic applications

Accurate, reliable, and cost-effective immunosensors are clinically important for the early diagnosis and monitoring of progressive diseases, and multiplexed sensing is a promising strategy for the next generation of diagnostics. This strategy allows for the simultaneous detection and quantification of multiple biomarkers with significantly enhanced reproducibility and reliability, whilst requiring smaller sample volumes, fewer materials, and shorter average analysis time for individual biomarkers than individual tests. In this opinionated review, we compare different techniques for the development of multiplexed immunosensors. We review the state-of-the-art approaches in the field of multiplexed immunosensors using electrical, electrochemical, and optical methods. The barriers that prevent translating this sensing strategy into clinics are outlined together with the potential solutions. We also share our vision on how multiplexed immunosensors will continue their evolution in the coming years