8,571 research outputs found
Role and importance of polymorphisms with respect to DNA methylation for the expression of CYP2E1 enzyme
Different individuals possess slightly different genetic information and show genetically-determined differences
in several enzyme activities due to genetic variability. Following an integrated approach,we studied the polymorphisms
andmethylation of sites contained in the 5′ flanking region of themetabolizing enzyme CYP2E1 in correlation
to its expression in both tumor and non-neoplastic liver cell lines, since to date little is known about the
influence of these (epi)genetic elements in basal conditions and under induction by the specific inductor and a
demethylating agent. In treated cells, reduced DNA methylation, assessed both at genomic and gene level, was
not consistently associatedwith the increase of enzyme expression. Interestingly, the Rsa/Pst haplotype differentially
influenced CYP2E1 enzyme expression. In addition, regarding the Variable Number of Tandem Repeats
polymorphism, cells with A4/A4 genotype showed a greater expression inhibition (ranging from 20% to 30%)
compared with others carrying the A2/A2 one, while those cells bringing A2/A3 genotype showed an increase
of expression (of 25%, about). Finally, we demonstrated for the first time that the A2 and A3 CYP2E1 alleles
play a more important role in the expression of the enzyme, compared with other (epi)genetic factors, since
they are binding sites for trans-acting proteins
Analysis of ASTEC-Na capabilities for simulating a loss of flow CABRI experiment
Abstract This paper presents simulation results of the CABRI BI1 test using the code ASTEC-Na, currently under development, as well as a comparison of the results with available experimental data. The EU-JASMIN project (7th FP of EURATOM) centres on the development and validation of the new severe accident analysis code ASTEC-Na (Accident Source Term Evaluation Code) for sodium-cooled fast reactors whose owner and developer is IRSN. A series of experiments performed in the past (CABRI/SCARABEE experiments) and new experiments to be conducted in the new experimental sodium facility KASOLA have been chosen to validate the developed ASTEC-Na code. One of the in-pile experiments considered for the validation of ASTEC-Na thermal–hydraulic models is the CABRI BI1 test, a pure loss-of-flow transient using a low burnup MOX fuel pin. The experiment resulted in a channel voiding as a result of the flow coast-down leading to clad melting. Only some fuel melting took place. Results from the analysis of this test using SIMMER and SAS-SFR codes are also presented in this work to check their suitability for further code benchmarking purposes
Experimental study and calculation of the electron transfer coefficients on the dissolution behavior of chitosan in organic acids
Chitosan (CH) consists of water-insoluble N-acetylglucosamine and D-glucosamine molecules and has a higher solubility at a pH below six. This studyevaluated the solubility of chitosan in solutions of organic acids for the formation of films. HyperChemTMsoftware was used to perform the quantum analysis. In the experimental trials, the total soluble mass (TSM) and the viscosity of the solutions were measured by capillary viscometer. The chitosan filmswere made by the plate melting method, and the filmcharacteristics were evaluated. A quantum simulation suggested that lactic acid (LA) has a greater stability to react with chitosan. It was then verified experimentally that LA is a better solvent for chitosan due to the increase in its viscosity. The chemical interaction between CH and LA in solution favors the polymerization of films with better physical properties. We thereforeconclude that the uniformity in the formation of films of this polymer depends on the chemical interaction between the CH and the acid and not on the degree of solubility of the polymer
The Effects of Cuento Therapy on Reading Achievement and Psychological Outcomes of Mexican-American Students
This investigation evaluated the effects of cuento therapy (an intervention using Spanish-language tales) on children’s self-esteem, affect, and reading test performance. The sample was composed of 58 third-grade Mexican-American students who were randomly assigned to the treatment and control groups. The results showed a mean self-esteem gain score difference between groups in favor of the treatment group for Global, Academic, and General self-esteem scores. Following intervention, the treatment group reported less physiological anxiety than did the control group. The results also showed mean increases in the pre- and post-standardized high-stakes reading test scores for both groups. Other significant findings regarding selfesteem and anxiety are reported
Decoherence from internal degrees of freedom for cluster of mesoparticles : a hierarchy of master equations
A mesoscopic evolution equation for an ensemble of mesoparticles follows
after the elimination of internal degrees of freedom. If the system is composed
of a hierarchy of scales, the reduction procedure could be worked repeatedly
and the characterization of this iterating method is carried out. Namely, a
prescription describing a discrete hierarchy of master equations for the
density operator is obtained. Decoherence follows from the irreversible
coupling of the systems, defined by mesoscopic variables, to internal degrees
of freedom. We discuss briefly the existence of systems with the same dynamics
laws at different scales. We made an explicit calculation for an ensemble of
particles with internal harmonic interaction in an external anharmonic field.
New conditions related to the semiclassical limit for mesoscopic systems
(Wigner-function) are conjectured.Comment: 19 pages, 0 figures, late
The Large Aperture GRB Observatory
The Large Aperture GRB Observatory (LAGO) is aiming at the detection of the
high energy (around 100 GeV) component of Gamma Ray Bursts, using the single
particle technique in arrays of Water Cherenkov Detectors (WCD) in high
mountain sites (Chacaltaya, Bolivia, 5300 m a.s.l., Pico Espejo, Venezuela,
4750 m a.s.l., Sierra Negra, Mexico, 4650 m a.s.l). WCD at high altitude offer
a unique possibility of detecting low gamma fluxes in the 10 GeV - 1 TeV range.
The status of the Observatory and data collected from 2007 to date will be
presented.Comment: 4 pages, proceeding of 31st ICRC 200
Water Cherenkov Detectors response to a Gamma Ray Burst in the Large Aperture GRB Observatory
In order to characterise the behaviour of Water Cherenkov Detectors (WCD)
under a sudden increase of 1 GeV - 1 TeV background photons from a Gamma Ray
Burst (GRB), simulations were conducted and compared to data acquired by the
WCD of the Large Aperture GRB Observatory (LAGO). The LAGO operates arrays of
WCD at high altitude to detect GRBs using the single particle technique. The
LAGO sensitivity to GRBs is derived from the reported simulations of the gamma
initiated particle showers in the atmosphere and the WCD response to
secondaries.Comment: 5 pages, proceeding of the 31st ICRC 200
Riesgo de diabetes mellitus tipo 2 choferes de automĂłvil de servicio pĂşblico de la ciudad de Lima
Letter to the Editor (without abstract)Carta al editor (sin resumen
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