Genetics of gene expression characterizes response to selective breeding for alcohol preference

Numerous selective breeding experiments have been performed with rodents, in an attempt to understand the genetic basis for innate differences in preference for alcohol consumption. Quantitative trait locus (QTL) analysis has been used to determine regions of the genome that are associated with the behavioral difference in alcohol preference/consumption. Recent work suggests that differences in gene expression represent a major genetic basis for complex traits. Therefore, the QTLs are likely to harbor regulatory regions (eQTLs) for the differentially expressed genes that are associated with the trait. In this study, we examined brain gene expression differences over generations of selection of the third replicate lines of high and low alcohol-preferring (HAP3 and LAP3) mice, and determined regions of the genome that control the expression of these differentially expressed genes (de eQTLs). We also determined eQTL regions (rv eQTLs) for genes that showed a decrease in variance of expression levels over the course of selection. We postulated that de eQTLs that overlap with rv eQTLs, and also with phenotypic QTLs, represent genomic regions that are affected by the process of selection. These overlapping regions controlled the expression of candidate genes (that displayed differential expression and reduced variance of expression) for the predisposition to differences in alcohol consumption by the HAP3/LAP3 mice

Free tensor multiplets and strings in spontaneously broken six-dimensional (2,0) theory

We first review the representations of the six-dimensional (2,0) superalgebra on a free tensor multiplet and on a free string. We then construct a supersymmetric Lagrangian describing a free tensor multiplet. (It also includes a decoupled anti self-dual part of the three-form field strength.) This field theory is then rewritten in variables appropriate for analyzing a situation where the R-symmetry is spontaneously broken by the vacuum expectation values of the scalar moduli fields. Finally, we construct a supersymmetric and kappa-symmetric action for a free string.Comment: 15 pages, LaTe

Improving Practice Accessibility Through MyChart Utilization

Aims for Improvement Increase patient portal MyChart activation for a cohort of approximately 2,000 patients at JFMA by 1% from February to April 2021. Demonstrate evidence of utilization including appointment scheduling, results review, and communication with providers through MyChart after activation

Arsenic occurrence in Malawi groundwater

Despite an estimated 90,000 groundwater points, mostly hand-pumped boreholes, being used for drinking-water supply in Malawi, evaluation of groundwater arsenic has been limited. Here we review the literature and collate archive data on groundwater arsenic occurrence in Malawi; add to these data, by surveying occurrence in handpumped boreholes in susceptible aquifers; and, conclude on risks to water supply. Published literature is sparse with two of the three studies reporting arsenic data in passing, with concentrations below detection limits. The third study of 25 alluvial aquifer boreholes found arsenic mostly at 1-10 μg/l concentration, but with four sites above the World Health Organisation (WHO) 10 μg/l drinking-water guideline, up to 15 μg/l; the study also discerned hydrochemical controls. Archive data from non-governmental organisation (NGO) borehole testing (two datasets) exhibited below detection results. Our surveys in 2014-18 of hand-pumped supplies in alluvial and bedrock aquifers tested 310 groundwater sites (78% alluvial, 22% bedrock) and found below test-kit detection (<10 μg/l) arsenic throughout, except possible traces at two boreholes containing geothermal-groundwater contributions. Our subsequent survey of 15 geothermal groundwater boreholes/springs found four sites with arsenic detected at 4-12 μg/l concentration. These sites displayed the highest temperatures, supporting increased arsenic being related to a geothermal groundwater influence. Our 919 sample dataset overall indicates arsenic in Malawian groundwater appears low, and well within Malawi’s drinking-water standard of 50 μg/l (MS733:2005). Still, however, troublesome concentrations above the WHO drinking-water guideline occur. Continued research is needed to confirm that human-health risks are low; including, increased monitoring of the great many hand-pumped supplies, and assessing hydro-biogeochemical controls on the higher arsenic concentrations found.Keywords: Arsenic; Groundwater quality; Malawi; Drinking wate

Relationship Between Cardiovascular Disease Knowledge and Race/Ethnicity, Education, and Weight Status

Background: Inadequate cardiovascular disease (CVD) knowledge has been cited to account for the imperfect decline in CVD among women over the last 2 decades. Hypothesis: Due to concerns that at-risk women might not know the leading cause of death or symptoms of a heart attack, our goal was to assess the relationship between CVD knowledge race/ethnicity, education, and body mass index (BMI). Methods: Using a structured questionnaire, CVD knowledge, socio-demographics, risk factors, and BMI were evaluated in 681 women. Results: Participants included Hispanic, 42.1% (n = 287); non-Hispanic white (NHW), 40.2% (n = 274); non-Hispanic black (NHB), 7.3% (n = 50); and Asian/Pacific Islander (A/PI), 8.7% (n = 59). Average BMI was 26.3 ± 6.1 kg/m2. Hypertension was more frequent among overweight (45%) and obese (62%) than normal weight (24%) (P 12 years (both P < 0.0001). Conclusions: Effective prevention strategies for at-risk populations need to escalate CVD knowledge and awareness among the undereducated and minority women

The effects of an experimental programme to support students’ autonomy on the overt behaviours of physical education teachers

Although the benefits of autonomy supportive behaviours are now well established in the literature, very few studies have attempted to train teachers to offer a greater autonomy support to their students. In fact, none of these studies has been carried out in physical education (PE). The purpose of this study is to test the effects of an autonomy-supportive training on overt behaviours of teaching among PE teachers. The experimental group included two PE teachers who were first educated on the benefits of an autonomy supportive style and then followed an individualised guidance programme during the 8 lessons of a teaching cycle. Their behaviours were observed and rated along 3 categories (i.e., autonomy supportive, neutral and controlling) and were subsequently compared to those of three teachers who formed the control condition. The results showed that teachers in the experimental group used more autonomy supportive and neutral behaviours than those in the control group, but no difference emerged in relation to controlling behaviours. We discuss the implications for schools of our findings