New strategy for the cleaning of paper artworks: A smart combination of gels and biosensors

n this work an outlook on the design and application, in the cultural heritage field, of new tools for diagnostic and cleaning use, based on biocompatible hydrogels and electrochemical sensors, is reported. The use of hydrogels is intriguing because it does not require liquid treatment that could induce damage on artworks, while electrochemical biosensors not only are easy to prepare, but also can be selective for a specific compound and therefore are suitable for monitoring the cleaning process. In the field of restoration of paper artworks, more efforts have to be done in order to know how to perform the best way for an effective restoration. Rigid Gellan gel, made up of Gellan gum and calcium acetate, was proposed as a paper cleaning treatment, and selective biosensors for substances to be removed from this gel have been obtained by choosing the appropriate enzymes to be immobilized. Using this approach, it is possible to know when the cleanup process will be completed, avoiding lengthy and sometimes unnecessary cleaning material applications

Pre-Production and Quality Assurance of the Mu2e Calorimeter Silicon Photomultipliers

The Mu2e electromagnetic calorimeter has to provide precise information on energy, time and position for $\sim$100 MeV electrons. It is composed of 1348 un-doped CsI crystals, each coupled to two large area Silicon Photomultipliers (SiPMs). A modular and custom SiPM layout consisting of a 3$\times$2 array of 6$\times$6 mm$^2$ UV-extended monolithic SiPMs has been developed to fulfill the Mu2e calorimeter requirements and a pre-production of 150 prototypes has been procured by three international firms (Hamamatsu, SensL and Advansid). A detailed quality assurance process has been carried out on this first batch of photosensors: the breakdown voltage, the gain, the quenching time, the dark current and the Photon Detection Efficiency (PDE) have been determined for each monolithic cell of each SiPMs array. One sample for each vendor has been exposed to a neutron fluency up to $\sim$8.5~$\times$~10$^{11}$ 1 MeV (Si) eq. n/cm$^{2}$ and a linear increase of the dark current up to tens of mA has been observed. Others 5 samples for each vendor have undergone an accelerated aging in order to verify a Mean Time To Failure (MTTF) higher than $\sim$10$^{6}$ hours.Comment: NDIP 2017 - New Developments In Photodetection, 3-7 July 2017, Tours (France

The Met80Ala and Tyr67His/Met80Ala mutants of human cytochrome cshed light on the reciprocal role of Met80 and Tyr67 in regulating ligand access into the heme pocket.

The spectroscopic and functional properties of the single Met80Ala and double Tyr67His/Met80Ala mutants of human cytochrome c have been investigated in their ferric and ferrous forms, and in the presence of different ligands, in order to clarify the reciprocal effect of these two residues in regulating the access of exogenous molecules into the heme pocket. In the ferric state, both mutants display an aquo high spin and a low spin species. The latter corresponds to an OH- ligand in Met80Ala but to a His in the double mutant. The existence of these two species is also reflected in the functional behavior of the mutants. The observation that (i) a significant peroxidase activity is present in the Met80Ala mutants, (ii) the substitution of the Tyr67 by His leads to only a slight increase of the peroxidase activity in the Tyr67His/Met80Ala double mutant with respect to wild type, while the Tyr67His mutant behaves as wild type, as previously reported, suggests that the peroxidase activity of cytochrome c is linked to an overall conformational change of the heme pocket and not only to the disappearance of the Fe-Met80 bond. Therefore, in human cytochrome c there is an interplay between the two residues at positions 67 and 80 that affects the conformation of the distal side of the heme pocket, and thus the sixth coordination of the hem

Gellan hydrogel as a powerful tool in paper cleaning process: a detailed study

Hypothesis: Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment. It is used to improve the optical qualities of a graphic work and remove dust and by-products resulting from cellulose degradation. Nevertheless, washing treatment usually involves a substantial impact on the original morphological structure of paper and can sometimes be dangerous for water sensitive inks and pigments. Experiments: The use of rigid hydrogel of Gellan gum as an alternative paper cleaning treatment is developed. The application of a rigid hydrogel minimizes damages caused by the use of water, and therefore is much more respectful for the original integrity of ancient paper. Findings: Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition. Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method

Inhibition of acetylpolyamine and spermine oxidases by the polyamine analogue chlorhexidine

""Acetylpolyamine and spermine oxidases are involved in the catabolism of polyamines. The discovery of selective. inhibitors of these enzymes represents an important tool for the development of novel anti-neoplastic drugs. Here, a. comparative study on acetylpolyamine and spermine oxidases inhibition by the polyamine analogue chlorhexidine. is reported. Chlorhexidine is an antiseptic diamide, commonly used as a bactericidal and bacteriostatic agent.. Docking simulations indicate that chlorhexidine binding to these enzymes is compatible with the stereochemical. properties of both acetylpolyamine oxidase and spermine oxidase active sites. In fact, chlorhexidine is predicted. to establish several polar and hydrophobic interactions with the active site residues of both enzymes, with binding. energy values ranging from −7.6 to −10.6 kcal\\\/mol. In agreement with this hypothesis, inhibition studies indicate that. chlorhexidine behaves as a strong competitive inhibitor of both enzymes, values of Ki being 0.10 μM and 0.55 μM for. acetylpolyamine oxidase and spermine oxidase, respectively."

The impact of SuperB on flavour physics

This report provides a succinct summary of the physics programme of SuperB, and describes that potential in the context of experiments making measurements in flavour physics over the next 10 to 20 years. Detailed comparisons are made with Belle II and LHCb, the other B physics experiments that will run in this decade. SuperB will play a crucial role in defining the landscape of flavour physics over the next 20 years.Comment: 20 pages, 6 figure

A new sustainable and innovative work for paper artworks cleaning process: Gellan hydrogel combined with hydrolytic enzymes

Paper has been used as writing and drawing support for thousands of years. The conservation of paper artworks plays a fundamental role in the field of our cultural heritage. Moreover, restoration of paper artworks is difficult due to their inherent fragility, the presence of many components and their degradation state. Among the factors that may contribute to paper deterioration are the use of glue for the application of different materials (as a lining, mounting or as a repair intervention) on the paper artifact. During a natural ageing process, glue become yellow, acid and less compact, accelerating the degradation processes of the artwork itself. The removal of glues from paper artworks represents, therefore, an important procedure for their preservation. Here we present a sustainable alternative to the common removal systems (e.g. solvents or localized enzymatic packs on the support to be cleaned). For this goal we used a rigid Gellan hydrogel (totally removable in one step) containing hydrolytic enzyme, such as proteinase K. The enzyme works as a selective cleaning agent hydrolyzing animal glues into smaller fragments, soluble into the gel. Our system represents an effective alternative to the traditional techniques because it is easy to be prepared, eco-friendly and efficient

The electromagnetic calorimeter of the AMS-02 experiment

The electromagnetic calorimeter (ECAL) of the AMS-02 experiment is a 3-dimensional sampling calorimeter, made of lead and scintillating fibers. The detector allows for a high granularity, with 18 samplings in the longitudinal direction, and 72 sampling in the lateral direction. The ECAL primary goal is to measure the energy of cosmic rays up to few TeV, however, thanks to the fine grained structure, it can also provide the separation of positrons from protons, in the GeV to TeV region. A direct measurement of high energy photons with accurate energy and direction determination can also be provided.Comment: Proceedings of SF2A conference 201

Design, status and perspective of the Mu2e crystal calorimeter

The Mu2e experiment at Fermilab will search for the charged lepton flavor violating process of neutrino-less $\mu \to e$ coherent conversion in the field of an aluminum nucleus. Mu2e will reach a single event sensitivity of about $2.5\cdot 10^{-17}$ that corresponds to four orders of magnitude improvements with respect to the current best limit. The detector system consists of a straw tube tracker and a crystal calorimeter made of undoped CsI coupled with Silicon Photomultipliers. The calorimeter was designed to be operable in a harsh environment where about 10 krad/year will be delivered in the hottest region and work in presence of 1 T magnetic field. The calorimeter role is to perform $\mu$/e separation to suppress cosmic muons mimiking the signal, while providing a high level trigger and a seeding the track search in the tracker. In this paper we present the calorimeter design and the latest R$\&$D results.Comment: 4 pages, conference proceeding for a presentation held at TIPP'2017. To be published on Springer Proceedings in Physic