Inflammation, neurotrophism and oxidative stress and childhood psychopathology in a large community sample

Objective: To investigate the association between peripheral biomarkers and child psychopathology in a large community sample. Method: A total of 625 aged 6- to 13-year old subjects were recruited from a community school-based study. Psychopathology was assessed using the Child Behaviour Checklist (CBCL). Psychiatric diagnosis was evaluated using the Development and Well-Being Assessment. The following biomarkers were examined in peripheral blood: brain-derived neurotrophic factor, cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, IFN-g, and TNF-alpha), chemokines (eotaxin/CCL11, IP-10, MCP-1), cytokine receptors (sTNFR1 and sTNFR2), and the oxidative stress marker TBARS. Results: We found significant associations between sTNFR2, eotaxin/CCL11 and CBCL total score, as well as with specific dimensions of psychopathology. There were different patterns of association between these biomarkers and psychological and behavioural symptoms in children with and without a mental disorder. TBARS, IL-6 and MCP-1 were more specific to some clusters of symptoms in children with a psychiatric diagnosis. Conclusion: Our data support the potential use of biomarkers, especially those involved in immune-inflammatory pathways, in investigating neurodevelopmental psychopathology. Their association with different dimensions of symptoms might be of useful when analyzing illness severity and clusters of symptoms within specific disorders.National Institute of Developmental Psychiatry for Children and Adolescents, a science and technology institute - Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP; Research Support Foundation of the State of Sao Paulo)Natl Inst Dev Psychiat INPD, Sao Paulo, BrazilFed Univ Sao Paulo UNIFESP, Dept Psychiat, Program Recognit & Intervent Individuals At Risk, Sao Paulo, BrazilFed Univ Sao Paulo UNIFESP, Dept Psychiat, Interdisciplinary Lab Clin Neurosci LINC, Sao Paulo, BrazilPontificial Catolic Univ Rio Grande do Sul PUCRS, Postgraduat Program Psychol, Porto Alegre, RS, BrazilPontificial Catolic Univ Rio Grande do Sul PUCRS, Dev Cognit Neurosci Res Grp GNCD, Porto Alegre, RS, BrazilFed Univ Rio Grande do Sul UFRGS, Mol Psychiat Unit, Porto Alegre, RS, BrazilFed Univ Rio Grande do Sul UFRGS, Natl Sci & Technol Inst Translat Med INCT TM, Porto Alegre, RS, BrazilUniv Texas Hlth Sci Ctr Houston, Dept Psychiat & Behav Sci, UT Ctr Mol Psychiat, Houston, TX 77030 USAUniv Fed Minas Gerais, Translat Psychoneuroimmunol Grp, Belo Horizonte, MG, BrazilFed Univ Rio Grande do Sul UFRGS, Lab Mol Psychiat, Porto Alegre, RS, BrazilUniv Sao Paulo, Fac Med, Dept Psychiat, Sao Paulo, BrazilFed Univ Sao Paulo UNIFESP, Dept Psychiat, Program Recognit & Intervent Individuals At Risk, Sao Paulo, BrazilFed Univ Sao Paulo UNIFESP, Dept Psychiat, Interdisciplinary Lab Clin Neurosci LINC, Sao Paulo, BrazilCNPq: 573974/2008-0FAPESP: 2008/57896-8Web of Scienc

Metabolomics and lipidomics analyses by 1H nuclear magnetic resonance of schizophrenia patient serum reveal potential peripheral biomarkers for diagnosis

Using 1H NMR-based metabolomics in association to chemometrics analysis, we analyzed here the metabolic differences between schizophrenia patients (SCZ) compared to healthy controls (HCs). HCs and SCZ patients underwent clinical interview using the Structured Clinical Interview for DSM Disorders (SCID). SCZ patients were further assessed by Positive and Negative Syndrome Scale (PANSS), Calgary Depression Scale, Global Assessment of Functioning Scale (GAF), and Clinical Global Impressions Scale (CGI). Using the principal component analysis (PCA) and supervised partial least-squares discriminate analysis (PLS-DA) in obtained NMR data, a clear group separation between HCs and SCZ patients was achieved. Interestingly, all metabolite compounds identified as exclusively present in the SCZ group, except for the gamma-aminobutyric acid (GABA), were never previously associated with mental disorders. Although the initial perception of an absence of obvious biological link among the different key molecules exclusively observed in each group, and no identification of any specific pathway yet, the present work represents an important contribution for the identification of potential biomarkers to inform diagnosis, as it was possible to completely separate the affected SCZ patients from HCs, with no outliers or exceptions. In addition, the data presented here reinforced the role of the modulation of glycolysis pathway and the loss of GABA interneuron/hyperglutamate hypothesis in SCZ. © 2016.Using 1H NMR-based metabolomics in association to chemometrics analysis, we analyzed here the metabolic differences between schizophrenia patients (SCZ) compared to healthy controls (HCs). HCs and SCZ patients underwent clinical interview using the Struct18518218

Serum copeptin in children exposed to maltreatment

AimChildhood maltreatment (CM) has been related to a persistent reprograming of stress-response. Copeptin is a marker of hypothalamic-pituitary-adrenal axis activationhowever, few studies have examined copeptin levels in children exposed to CM. The aim of this study was to compare serum copeptin levels in children reporting child abuse and/or neglect and children with no history of CM. MethodsThis study included 65 children with a positive history of moderate to severe CM, as reported by themselves and their parent(s) during a clinical interview, and 71 children with no history of CM as a comparison group. CM was considered moderate to severe based on the child-reported frequency of being exposed to events related to sexual abuse, physical abuse, emotional abuse, emotional neglect, and/or physical neglect. Child psychopathology symptoms were assessed using the Child Behavior Checklist (CBCL). We measured serum copeptin concentration using enzyme-linked immunosorbent assay. ResultsChildren exposed to CM exhibited higher levels of serum copeptin compared to children without CM when controlling for sex, age, and psychiatric morbidity. The CBCL total score, including internalizing and externalizing symptoms, was higher in children with CM. We found no correlation between copeptin and CBCL scores for internalizing symptoms and externalizing symptoms. Conclusion CM is associated with copeptin serum levels independently of age, sex, and symptom severity. Copeptin is a promising new biomarker for children with a history of abuse and/or neglect.National Institute of Developmental Psychiatry for Children and Adolescents, a science and technology instituteConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Pontifical Catholic Univ Rio Grande Sul PUCRS, DCNL, Ave Ipiranga 6681,Predio 11,Sala 928 Partenon, BR-90619900 Porto Alegre, RS, BrazilUniv Fed Rio Grande do Sul, Dept Psychiat, Porto Alegre, RS, BrazilCNPq, Natl Inst Dev Psychiat Children & Adolescents, Sao Paulo, BrazilFed Univ Sao Paulo UNIFESP, Dept Psychiat, Sao Paulo, BrazilUniv Fed Sao Paulo, Interdisciplinary Lab Clin Neurosci LINC, Sao Paulo, BrazilUniv Sao Paulo, Inst Psychiat IPq, Sao Paulo, BrazilUniv Toronto, Univ Hlth Network, MDPU, Toronto, ON, CanadaDepartment of Psychiatry, Universidade Federal de São Paulo (UNIFESP), Sao Paulo, BrazilInterdisciplinary Laboratory of Clinical Neurosciences (LINC), Universidade Federal de São Paulo (UNIFESP), Sao Paulo, BrazilCNPq: 573974/2008-0CNPq: 305141/2011-2CNPq: 476468/ 2012-4FAPESP: 2008/57896-8Web of Scienc