20 research outputs found

    Efeito da suplementação com ferro na biodisponibilidade de zinco em uma dieta regional do nordeste do Brasil

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    Foram investigados os efeitos da suplementação com ferro na biodisponibilidade de zinco de uma dieta "regional" do Nordeste (DRNE), em ratos albinos Wistar, consumindo raçÔes Ă  base da referida dieta (DRNE) e raçÔes controle. As raçÔes DRNE, continham 16 mg de Zn/kg e nĂ­veis de 35 mg, 70 mg e 140mg Fe/kg. As raçÔes controle foram elaboradas segundo o "Committee on Laboratory Animal Diets", contendo nĂ­veis de proteĂ­na, ferro e zinco ajustados aos das raçÔes experimentais DRNE. Os parĂąmetros utilizados para medir a biodisponibilidade do zinco foram: Índice de Absorção Aparente do Zn e nĂ­vel total de Zn nos fĂȘmures. Os resultados obtidos demonstraram que a suplementação com ferro diminuiu a biodisponibilidade do Zn, e os efeitos dessa interferĂȘncia foram influenciados pela qualidade da dieta e pelas proporçÔes Fe:Zn. Tal fato deve ser considerado nas prĂĄticas que envolvem fortificação de alimentos e/ou suplementos medicamentosos, comuns nas populaçÔes com carĂȘncias nutricionais.The effects of supplementation with iron on the zinc bioavailability of the regional diet of northeastern Brazil (RDN), were investigated. One assay with Wistar rats, feed on RDN and control diets was carried out. The RDN diets contained 16 mg Zn/kg and levels of 35 mg, 70 mg and 140 mg Fe/kg, respectively. The control diets were prepared according to the standards of the Committee on Laboratory Animal Diets, with levels of protein, iron and zinc identical to those of RDN diets. Index of apparent absorption and zinc retained in the femur of the animals were the parameters utilized to measure zinc bioavailability. The results demonstrated that the supplementation with iron decreased the zinc bioavailability, and the effects were seen to affect diet quality and the Fe:Zn ratio. This fact must be taken into consideration in practices such as the fortying of foodstuffs and the administration of vitamin-mineral supplements to populations with nutritional deficiencies

    Purification of a lectin from Cratylia mollis crude extract seed by a single step PEG/phosphate aqueous two-phase system

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    The partitioning and purification of lectins from the crude extract of Cratylia mollis seeds (Cramoll 1,4) was investigated in aqueous two-phase systems (ATPS). A factorial design model (24) was used to evaluate the influence of polyethylene glycol (PEG) molar mass (15008000g/mol), PEG concentration (12.517.5% w/w), phosphate (1015% w/w) concentration, and pH (68) on the differential partitioning, purification factor, and yield of the lectin. Polymer and salt concentration were the most important variables affecting partition of lectin and used to find optimum purification factor by experimental BoxBehnken design together with the response surface methodology (RSM). ATPS showed best conditions composed by 13.9% PEG1500, 15.3% phosphate buffer at pH 6, which ensured purification factor of 4.70. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis showed a single band of protein with 26.1kDa. Furthermore, results demonstrated a thermostable lectin presenting activity until 60°C and lost hemagglutinating activity at 80°C. According to the obtained data it can be inferred that the ATPS optimization using RSM approach can be applied for recovery and purification of lectins.We are grateful to the following bodies for the grants awarded: CAPES (Coordination for the Improvement of Level Personnel Superior); FACEPE (Pernambuco Science and Technology Foundation): Researcher's scholarship grant: BFP-0017-5.05/18 CNPq (National Council for Scientific Development and Technological) process: 427153/2016-6 and we also thank the reviewers for their valuable comments and suggestions as these helped us to improve the manuscript.info:eu-repo/semantics/publishedVersio

    Genome of Herbaspirillum seropedicae Strain SmR1, a Specialized Diazotrophic Endophyte of Tropical Grasses

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    The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes and plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable of colonizing intercellular spaces of grasses such as rice and sugar cane. The genome of H. seropedicae strain SmR1 was sequenced and annotated by The Paraná State Genome Programme—GENOPAR. The genome is composed of a circular chromosome of 5,513,887 bp and contains a total of 4,804 genes. The genome sequence revealed that H. seropedicae is a highly versatile microorganism with capacity to metabolize a wide range of carbon and nitrogen sources and with possession of four distinct terminal oxidases. The genome contains a multitude of protein secretion systems, including type I, type II, type III, type V, and type VI secretion systems, and type IV pili, suggesting a high potential to interact with host plants. H. seropedicae is able to synthesize indole acetic acid as reflected by the four IAA biosynthetic pathways present. A gene coding for ACC deaminase, which may be involved in modulating the associated plant ethylene-signaling pathway, is also present. Genes for hemagglutinins/hemolysins/adhesins were found and may play a role in plant cell surface adhesion. These features may endow H. seropedicae with the ability to establish an endophytic life-style in a large number of plant species

    Perfil lipídico e peroxidação de lipídeos no plasma em crianças e adolescentes com sobrepeso e obesidade

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    OBJETIVO: Identificar o perfil lipĂ­dico e a peroxidação de lipĂ­deos no plasma de crianças e adolescentes com sobrepeso e obesidade atendidos no AmbulatĂłrio de Endocrinologia PediĂĄtrica do HOSPED/UFRN. MÉTODOS: Foram constituĂ­dos grupos com crianças e adolescentes com sobrepeso (n = 15), obesidade (n = 30) e controle (n = 21). O perfil lipĂ­dico foi avaliado por meio do colesterol total, LDL-colesterol, HDL-colesterol e triglicerĂ­deos. A peroxidação de lipĂ­deos no plasma foi medida pelo marcador malonildialdeĂ­do (MDA). A anĂĄlise estatĂ­stica foi realizada atravĂ©s do teste t de Student, teste de Tukey, ANOVA e correlação de Pearson. RESULTADOS: As alteraçÔes de colesterol total e LDL-colesterol estavam mais presentes nos grupos sobrepeso e obesidade masculinos. O HDL-colesterol mostrou-se em condiçÔes limĂ­trofes nos grupos sobrepeso e obesidade em ambos sexos. As maiores concentraçÔes de triglicerĂ­deos foram registradas no grupo obesidade feminino. Identificou-se elevada peroxidação de lipĂ­deos no plasma no grupo obesidade, principalmente no sexo masculino. CONCLUSÕES: Na amostra estudada, maiores alteraçÔes do perfil lipĂ­dico foram observadas no sexo masculino nos grupos sobrepeso e obesidade, e a peroxidação de lipĂ­deos estava mais evidente no grupo obesidade em ambos sexos

    Efeito da suplementação com ferro na biodisponibilidade de zinco em uma dieta regional do nordeste do Brasil Effects of supplementation with iron on the bioavailability of zinc in the regional diet of northeastern Brazil

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    Foram investigados os efeitos da suplementação com ferro na biodisponibilidade de zinco de uma dieta "regional" do Nordeste (DRNE), em ratos albinos Wistar, consumindo raçÔes Ă  base da referida dieta (DRNE) e raçÔes controle. As raçÔes DRNE, continham 16 mg de Zn/kg e nĂ­veis de 35 mg, 70 mg e 140mg Fe/kg. As raçÔes controle foram elaboradas segundo o "Committee on Laboratory Animal Diets", contendo nĂ­veis de proteĂ­na, ferro e zinco ajustados aos das raçÔes experimentais DRNE. Os parĂąmetros utilizados para medir a biodisponibilidade do zinco foram: Índice de Absorção Aparente do Zn e nĂ­vel total de Zn nos fĂȘmures. Os resultados obtidos demonstraram que a suplementação com ferro diminuiu a biodisponibilidade do Zn, e os efeitos dessa interferĂȘncia foram influenciados pela qualidade da dieta e pelas proporçÔes Fe:Zn. Tal fato deve ser considerado nas prĂĄticas que envolvem fortificação de alimentos e/ou suplementos medicamentosos, comuns nas populaçÔes com carĂȘncias nutricionais.<br>The effects of supplementation with iron on the zinc bioavailability of the regional diet of northeastern Brazil (RDN), were investigated. One assay with Wistar rats, feed on RDN and control diets was carried out. The RDN diets contained 16 mg Zn/kg and levels of 35 mg, 70 mg and 140 mg Fe/kg, respectively. The control diets were prepared according to the standards of the Committee on Laboratory Animal Diets, with levels of protein, iron and zinc identical to those of RDN diets. Index of apparent absorption and zinc retained in the femur of the animals were the parameters utilized to measure zinc bioavailability. The results demonstrated that the supplementation with iron decreased the zinc bioavailability, and the effects were seen to affect diet quality and the Fe:Zn ratio. This fact must be taken into consideration in practices such as the fortying of foodstuffs and the administration of vitamin-mineral supplements to populations with nutritional deficiencies

    Cirandas da vida: dialogismo e arte na gestĂŁo em saĂșde Cirandas da vida: dialogism and art within health management

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    Este estudo tem como objeto as açÔes das Cirandas da Vida, em seu dialogismo entre o princĂ­pio da comunidade e a esfera institucional na formulação e implementação de polĂ­ticas de saĂșde e tenta apreender como a perspectiva popular se expressa com arte na gestĂŁo em saĂșde. Busca capturar como as comunidades exprimem sua histĂłria de luta, mediante as linguagens da arte como fertilizadora do princĂ­pio de comunidade; analisar como os atores populares se inserem na formulação e implementação dessas polĂ­ticas; compreender como os diferentes grupos geracionais expressam suas leituras da realidade, no dialogismo vivido na gestĂŁo em saĂșde e analisar como as linguagens da arte contribuem para a construção de atos limite como estratĂ©gias de superação das situaçÔes-limite apontadas nas rodas das Cirandas. Trabalhamos com a pesquisa ação, constituindo uma comunidade ampliada de pesquisa que procedeu Ă  anĂĄlise coletiva da experiĂȘncia. A violĂȘncia aponta como uma das situaçÔes limite, revelando o seu impacto na juventude em situação de conflito com a lei e as linguagens da artes surgem como potĂȘncias, espaços de expressĂŁo, problematização e transgressĂŁo da realidade e do envolvimento desses jovens na construção de polĂ­ticas. Revela ainda o enfoque de gĂȘnero e as estratĂ©gias de sĂłcio-economia-solidĂĄria, bem como prĂĄticas de cuidado como atos-limite. O acesso aos serviços de saĂșde, outra situação limite apontada, revelou a necessidade de fortalecer a humanização da atenção. A arte e as prĂĄticas populares de cuidado surgem como potĂȘncias dos atores populares que ocuparam rodas de gestĂŁo, incluindo seus saberes no cotidiano: o inĂ©dito viĂĄvel.<br>This study discusses the actions of the project 'Cirandas da Vida', which works indialogism between the principles of community and the institutional field in the formulation and implementation of health policies. It tries to understand how popular perspective expresses itself artistically within health management, and to capture how communities express their history of fight through artsÂŽ languages as a fertilizer of the principle of community; we looked into how popular actors insert themselves into the formulation and implementation of these policies, in order to understand how different groups of different generations express their readings of reality, in the dialogism lived within health management. Finally, we try and analyze how artsÂŽ languages contribute to the construction of limit acts as strategies to surpass limit situations which came up during Cirandas' encounters . Action research was the chosen method: an extended research community was constituted and proceeded to a collective analysis of the experience. Violence points out that it is one of the limit situations, revealing its impact onto youth with problems with the law and the potential of the languages of art as spaces of expression, problematization and transgression of reality and the involvement of these youngsters within the construction of policies. It reveals also the focus on gender, and the strategies of social and solidarity economy and care practices as limit acts. Access to health services was another limit situation pointed out, emphasizing the necessity of strengthening humanization in health attention. Art and popular practices of care emerge as potentialities of the popular actors that participated of the management encounters, bringing their knowledge in a daily basis: the viable unknown
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