The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression

abstract: The brown brocket deer is a species of neotropical deer that is found in several regions of Brazil. Although not considered an endangered species, it can be used as a model for studies with somatic cell nuclear transfer (SCNT) in endangered species. Thus, this study aimed to verify the embryo development and nuclear gene expression in intraSCNT (bovine-bovine), interSCNT (deer-bovine) and in vitro fertilized (IVF) bovine embryos. Thus, from a culture of brown brocket deer fibroblasts and enucleated bovine oocytes, inter and intraspecific embryos were produced. In addition, a group of IVF bovine embryos was prepared. Quantitative PCR (qPCR) was performed from the blastocysts obtained from each experimental group to analyze the expression of OCT4, T-FAM, GJA1, BAX and BCL2. The blastocyst rate was higher (P  0.05) among experimental groups. In conclusion, bovine cytoplast was able to reprogram the brown brocket deer karyoplast and these cloned embryos were able to reach the blastocyst stage. The inverse relationship for BAX and BCL2 can indicate a high degree of apoptosis in blastocysts from interSCNT

Matching metal pollution with bioavailability, bioaccumulation and biomarkers response in fish (Centropomus parallelus) resident in neotropical estuaries

Two neotropical estuaries affected by different anthropogenic factors were studied. We report levels of metals and metalloids in water and sediment as well as their influence on genetic, biochemical and morphological biomarkers in the native fish Centropomus parallelus. Biomarkers reflected the fish health status. Multivariate statistics indicated both spatial and temporal changes in both water and sediment, which are linked to the elemental composition and health status of inhabitant fish, showing the biggest influence of surface water, followed by sediments and interstitial water. Bioaccumulation in fish muscle was useful to identify elements that were below detection limits in water, pointing out the risk of consuming fish exceeding allowance limits for some elements (As and Hg in this case). Multivariate statistics, including physical, chemical and biological issues, presents a suitable tool, integrating data from different origin allocated in the same estuary, which could be useful for future studies on estuarine systems.Fil: Souza, Iara C.. Universidade Federal do Sao Carlos; BrasilFil: Duarte, Ian D.. Universidade Federal do Espírito Santo; BrasilFil: Pimentel, Natieli Q.. Universidade Federal do Sao Carlos; BrasilFil: Rocha, Lívia D.. Universidade Federal do Espírito Santo; BrasilFil: Morozesk, Mariana. Universidade Federal do Espírito Santo; BrasilFil: Bonomo, Marina M.. Universidade Federal do Espírito Santo; BrasilFil: Azevedo, Vinicius C.. Universidade Federal do Sao Carlos; BrasilFil: Pereira, Camilo D.S.. Universidade Santa Cecília; BrasilFil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Milanez, Camilla R. D.. Universidade Federal do Espírito Santo; BrasilFil: Matsumoto, Silvia T.. Universidade Federal do Espírito Santo; BrasilFil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Fernandes, Marisa N.. Universidade Federal do Sao Carlos; Brasi